Construct: ORF ccsbBroad304_12290

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007630.1_s304c1, BRDN0000404675
Derived from:
ccsbBroadEn_12290
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MYO5C (55930)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12290
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55930 MYO5C myosin VC XM_017022408.2 25.3% 24.9% 1005T>C;1273_1312del;1331_5091del
2 human 55930 MYO5C myosin VC XM_011521781.3 25% 24.7% 1005T>C;1273_1312del;1331_5142del
3 human 55930 MYO5C myosin VC NM_018728.4 24.6% 24.3% 1005T>C;1273_1312del;1331_5226del
4 mouse 208943 Myo5c myosin VC XR_001778836.1 30.7% (many diffs)
5 mouse 208943 Myo5c myosin VC XM_006510940.3 30% 32.4% (many diffs)
6 mouse 208943 Myo5c myosin VC XM_006510939.2 28.8% 31% (many diffs)
7 mouse 208943 Myo5c myosin VC NM_001081322.1 21.4% 23.1% (many diffs)
8 mouse 208943 Myo5c myosin VC XM_006510935.3 21.3% 23% (many diffs)
9 mouse 208943 Myo5c myosin VC XM_006510936.3 21.3% 23% (many diffs)
10 mouse 208943 Myo5c myosin VC XM_006510937.3 21.3% 23% (many diffs)
11 mouse 208943 Myo5c myosin VC XM_011242693.2 21.3% 23% (many diffs)
12 mouse 208943 Myo5c myosin VC XR_001778835.1 20.6% (many diffs)
13 mouse 208943 Myo5c myosin VC XR_379407.3 16.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1356
ORF length:
1290
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ggtggccgag ctgtacacgc agtacaacag ggtctggatt cccgatcctg
121aagaagtttg gaagtctgct gaaatagcca aggactacag agttggtgac aaggtcctgc
181gactcctgct ggaggatgga acggagctgg attattctgt caatccagaa tctctgcctc
241cacttcggaa tcctgacatc ctcgtgggcg agaatgacct cacggctctc agctatcttc
301acgagcccgc ggtgctccac aacctcagaa tccgctttgc agaatccaaa ctcatttaca
361cctacagtgg aatcattttg gtggccatga atccttacaa gcagttgcca atatacggag
421atgccatcat ccacgcctac agcgggcaga acatgggcga tatggaccca cacatatttg
481ccgtggcaga agaggcatac aagcagatgg ccagaaacaa cagaaaccag tccataattg
541taagtgggga gtcaggtgct ggaaagacag tgtcggctcg ctatgccatg aggtactttg
601ccaccgtcag caaatcgggc agcaacgctc acgtggaaga caaggtcctg gcatccaatc
661ccatcaccga ggccgttgga aatgccaaga ccacccgcaa tgacaatagt agtcggtttg
721ggaaatacac agaaatcagt tttgatgaac aaaatcaaat tataggagcc aacatgagca
781cttacctcct ggagaaatcc agagttgtct ttcaatcgga aaatgaacga aattaccaca
841ttttctatca gctttgtgca tctgcacagc agtcggaatt taaacatctt aaattgggga
901gtgccgaaga atttaattat acaagaatgg gaggcaatac tgtcattgag ggtgtgaatg
961atcgagctga aatggtagag actcaaaaga ccttcacgct tctgggtttc aaggaggatt
1021ttcagatgga cgtttttaaa atcctggcag ccatcctaca tctgggcaac gtgcagatca
1081ccgcggtggg caacgagagg tcctcagtta gtgaggatga cagtcacctg aaggtgttct
1141gtgagctcct gggcctggag agtggcagag ttgctcagtg gctgtgcaat cgcaaaatcg
1201tcacaagctc tgagacggtg gtaaaaccca tgaccaggcc tcaggctgtc aacgccaggg
1261atgcactggc caaaaagatc tatgctcacc tgttcgactt cattgtggag agaattaacc
1321aagcgttgca gttttcagtt ttgaaacctt tgatgtgccc aactttcttg tacaaagtgg
1381ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
1441ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

Download FASTA (ORF) (Full)