Transcript: Human XM_011521781.3

PREDICTED: Homo sapiens myosin VC (MYO5C), transcript variant X1, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MYO5C (55930)
Length:
6891
CDS:
163..5307

Additional Resources:

NCBI RefSeq record:
XM_011521781.3
NBCI Gene record:
MYO5C (55930)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011521781.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000162244 CAGTTGCCAATATACGGAGAT pLKO.1 499 CDS 100% 4.050 5.670 N MYO5C n/a
2 TRCN0000160881 GCAGGACAAGTGGCTTATTTA pLKO.1 2386 CDS 100% 15.000 12.000 N MYO5C n/a
3 TRCN0000278547 GCAGGACAAGTGGCTTATTTA pLKO_005 2386 CDS 100% 15.000 12.000 N MYO5C n/a
4 TRCN0000159318 GCCAACATATTCACCATAATT pLKO.1 6319 3UTR 100% 15.000 10.500 N MYO5C n/a
5 TRCN0000278494 GCCAACATATTCACCATAATT pLKO_005 6319 3UTR 100% 15.000 10.500 N MYO5C n/a
6 TRCN0000158936 GCTATGACATTGAAGATGTAA pLKO.1 3416 CDS 100% 5.625 3.938 N MYO5C n/a
7 TRCN0000278495 GCTATGACATTGAAGATGTAA pLKO_005 3416 CDS 100% 5.625 3.938 N MYO5C n/a
8 TRCN0000159847 GCTACTTAGAAGAATGGCTTA pLKO.1 4910 CDS 100% 4.050 2.835 N MYO5C n/a
9 TRCN0000297474 GCTACTTAGAAGAATGGCTTA pLKO_005 4910 CDS 100% 4.050 2.835 N MYO5C n/a
10 TRCN0000162536 CCTGTATCAGTTGATTCGCAT pLKO.1 2553 CDS 100% 2.640 1.848 N MYO5C n/a
11 TRCN0000278493 CCTGTATCAGTTGATTCGCAT pLKO_005 2553 CDS 100% 2.640 1.848 N MYO5C n/a
12 TRCN0000072628 CCTCCCAAAGTGCTAGGATTA pLKO.1 6130 3UTR 100% 10.800 5.400 Y MRPS16 n/a
13 TRCN0000140657 CCTCCCAAAGTGCTAGGATAA pLKO.1 6130 3UTR 100% 10.800 5.400 Y CD3EAP n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011521781.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12290 pDONR223 100% 25% 24.7% None 1005T>C;1273_1312del;1331_5142del n/a
2 ccsbBroad304_12290 pLX_304 0% 25% 24.7% V5 1005T>C;1273_1312del;1331_5142del n/a
3 TRCN0000475184 GATTGAAATTTTCATGTTTAGAGT pLX_317 32.3% 25% 24.7% V5 1005T>C;1273_1312del;1331_5142del n/a
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