Construct: ORF ccsbBroad304_12327
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005427.1_s304c1, BRDN0000402696
- Derived from:
- ccsbBroadEn_12327
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- PCNP (57092)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12327
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57092 | PCNP | PEST proteolytic signal con... | NM_001320395.1 | 100% | 100% | |
| 2 | human | 57092 | PCNP | PEST proteolytic signal con... | XM_017006922.1 | 99.2% | 99.2% | 279_280insAAG |
| 3 | human | 57092 | PCNP | PEST proteolytic signal con... | NM_001320400.1 | 80.7% | 80.7% | 278_279ins75 |
| 4 | human | 57092 | PCNP | PEST proteolytic signal con... | NM_020357.3 | 73% | 62.7% | 355_410del;447_534del |
| 5 | human | 57092 | PCNP | PEST proteolytic signal con... | NM_001320398.1 | 72.4% | 62.2% | 279_280insAAG;352_407del;444_531del |
| 6 | human | 57092 | PCNP | PEST proteolytic signal con... | NM_001320401.1 | 71.6% | 66% | (many diffs) |
| 7 | human | 57092 | PCNP | PEST proteolytic signal con... | NM_001320397.1 | 64.7% | 45.2% | (many diffs) |
| 8 | human | 57092 | PCNP | PEST proteolytic signal con... | NM_001320399.1 | 56.4% | 44.2% | (many diffs) |
| 9 | human | 57092 | PCNP | PEST proteolytic signal con... | NR_135232.1 | 15.1% | (many diffs) | |
| 10 | human | 57092 | PCNP | PEST proteolytic signal con... | NR_135227.1 | 15% | (many diffs) | |
| 11 | human | 57092 | PCNP | PEST proteolytic signal con... | NR_135228.1 | 14.5% | 1_122del;184_185ins52;461_2279del | |
| 12 | human | 57092 | PCNP | PEST proteolytic signal con... | NR_135223.1 | 14.1% | (many diffs) | |
| 13 | human | 57092 | PCNP | PEST proteolytic signal con... | NR_135225.1 | 14% | (many diffs) | |
| 14 | human | 57092 | PCNP | PEST proteolytic signal con... | NR_135230.1 | 12.7% | (many diffs) | |
| 15 | human | 57092 | PCNP | PEST proteolytic signal con... | NR_135226.1 | 11.2% | (many diffs) | |
| 16 | mouse | 76302 | Pcnp | PEST proteolytic signal con... | NM_001024622.2 | 67.9% | 58.5% | (many diffs) |
| 17 | mouse | 76302 | Pcnp | PEST proteolytic signal con... | XM_006522697.3 | 67.4% | 57.9% | (many diffs) |
| 18 | mouse | 76302 | Pcnp | PEST proteolytic signal con... | XM_011246041.2 | 52.3% | 41% | (many diffs) |
| 19 | mouse | 76302 | Pcnp | PEST proteolytic signal con... | XM_006522695.3 | 43.8% | 35% | (many diffs) |
| 20 | mouse | 76302 | Pcnp | PEST proteolytic signal con... | XM_006522696.3 | 43.4% | 34.6% | (many diffs) |
| 21 | mouse | 76302 | Pcnp | PEST proteolytic signal con... | XM_017317156.1 | 31.2% | 23.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 456
- ORF length:
- 390
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggacgggaag gcgggagacg agaagcctga aaagtcgcag cgagctggag 121 ccgccggagg acctgaagaa gaagcagaaa aacctgtgaa aactaagact gtttcttcca 181 gtaatggagg ggaaagttcc agtcgcagcg ctgagaagcg atcagctgaa gaagaagctg 241 ccgacctccc aacaaagcct acaaagatct ccaagtttgg atttgccata ggtagtcaga 301 cgacaaagaa agcatcagcc atatccatca aacttggatc aagtaagcct aaagaaactg 361 ttccaactct tgctccaaaa actctttcag tagcagcagc ttttaatgaa gatgaagatg 421 gatacaccaa catcagctgg accaaactcc ttcaataccc aactttcttg tacaaagtgg 481 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 541 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta