Construct: ORF ccsbBroad304_12394
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001883.1_s304c1, BRDN0000411033
- Derived from:
- ccsbBroadEn_12394
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SHTN1 (57698)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12394
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 57698 | SHTN1 | shootin 1 | NM_001258298.1 | 21.8% | 21.8% | 1_1338del |
| 2 | human | 57698 | SHTN1 | shootin 1 | NM_001127211.3 | 19.8% | 19.8% | 1_1518del |
| 3 | human | 57698 | SHTN1 | shootin 1 | XM_005270002.4 | 9.2% | 8.5% | (many diffs) |
| 4 | human | 57698 | SHTN1 | shootin 1 | NM_001258300.1 | 9% | 9.1% | 1_1338del;1494_1494delAins220 |
| 5 | human | 57698 | SHTN1 | shootin 1 | NM_001258299.1 | 8.1% | 8.2% | 1_1518del;1674_1674delAins220 |
| 6 | mouse | 71653 | Shtn1 | shootin 1 | NM_001114312.1 | 17.4% | 15.5% | (many diffs) |
| 7 | mouse | 71653 | Shtn1 | shootin 1 | XR_386512.3 | 7.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 441
- ORF length:
- 375
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc agtgttgggt tctgtatcca gtgtaacaaa aacagccttg aacaagaaaa 121 ctctggaggc agaattcaac agcccgtccc ccccaacacc tgagccaggt gaagggcccc 181 gtaaattgga aggatgcaca agttccaagg ttacgtttca gcctcccagt agcattggat 241 gcaggaaaaa atacattgac ggtgaaaaac aagccgaacc agttgtagtt ttagatcctg 301 tttctacaca tgaaccccaa accaaagacc aggttgctga aaaagatcca actcaacaca 361 aggaggatga aggcgaaatt caaccagaaa acaaagaaga cagcattgaa aacgtgagag 421 agacagacag ctccaactgc tgcccaactt tcttgtacaa agtggttggt aagcctatcc 481 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 541 ttaagtcgac aatcaacctc tggatta