Construct: ORF ccsbBroad304_12459

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014340.1_s304c1, BRDN0000406212
Derived from:
ccsbBroadEn_12459
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MMS19 (64210)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12459
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 64210 MMS19 MMS19 homolog, cytosolic ir... XM_017016534.2 47.6% 47.7% 1_963del;1326C>T
2 human 64210 MMS19 MMS19 homolog, cytosolic ir... XM_017016535.2 47.6% 47.7% 1_963del;1326C>T
3 human 64210 MMS19 MMS19 homolog, cytosolic ir... XM_011540063.3 47.5% 47.6% 1_966del;1329C>T
4 human 64210 MMS19 MMS19 homolog, cytosolic ir... XM_017016533.2 44% 44% 1_1116del;1479C>T
5 human 64210 MMS19 MMS19 homolog, cytosolic ir... XM_024448126.1 44% 44% 1_1116del;1479C>T
6 human 64210 MMS19 MMS19 homolog, cytosolic ir... NM_001351358.1 43.9% 43.9% 1_1119del;1482C>T
7 human 64210 MMS19 MMS19 homolog, cytosolic ir... NM_001351359.1 43.9% 43.9% 1_1119del;1482C>T
8 human 64210 MMS19 MMS19 homolog, cytosolic ir... XM_006717945.3 43.9% 43.9% 1_1119del;1482C>T
9 human 64210 MMS19 MMS19 homolog, cytosolic ir... XM_017016527.2 43.9% 43.9% 1_1119del;1482C>T
10 human 64210 MMS19 MMS19 homolog, cytosolic ir... XM_017016529.2 43.9% 43.9% 1_1119del;1482C>T
11 human 64210 MMS19 MMS19 homolog, cytosolic ir... XM_017016531.1 43.9% 43.9% 1_1119del;1482C>T
12 human 64210 MMS19 MMS19 homolog, cytosolic ir... XM_024448123.1 43.9% 43.9% 1_1119del;1482C>T
13 human 64210 MMS19 MMS19 homolog, cytosolic ir... XM_024448124.1 43.9% 43.9% 1_1119del;1482C>T
14 human 64210 MMS19 MMS19 homolog, cytosolic ir... XM_024448125.1 43.9% 43.9% 1_1119del;1482C>T
15 human 64210 MMS19 MMS19 homolog, cytosolic ir... XM_005270041.1 35.3% 35.3% 1_1608del;1971C>T
16 human 64210 MMS19 MMS19 homolog, cytosolic ir... XM_011540062.1 35.3% 35.3% 1_1608del;1971C>T
17 human 64210 MMS19 MMS19 homolog, cytosolic ir... NM_001289404.1 33.6% 33.6% 1_1734del;2097C>T
18 human 64210 MMS19 MMS19 homolog, cytosolic ir... XM_017016524.2 33.6% 33.6% 1_1734del;2097C>T
19 human 64210 MMS19 MMS19 homolog, cytosolic ir... NM_001351357.1 33.5% 33.6% 1_1737del;2100C>T
20 human 64210 MMS19 MMS19 homolog, cytosolic ir... XM_005270035.3 33.5% 33.6% 1_1737del;2100C>T
21 human 64210 MMS19 MMS19 homolog, cytosolic ir... XM_017016522.1 33.5% 33.6% 1_1737del;2100C>T
22 human 64210 MMS19 MMS19 homolog, cytosolic ir... XM_017016521.2 31.4% 31.4% 1_1914del;2277C>T
23 human 64210 MMS19 MMS19 homolog, cytosolic ir... NM_001330128.1 31.4% 31.4% 1_1917del;2280C>T
24 human 64210 MMS19 MMS19 homolog, cytosolic ir... XM_017016519.2 30.1% 30.1% 1_2034del;2397C>T
25 human 64210 MMS19 MMS19 homolog, cytosolic ir... XM_017016518.2 29.6% 29.7% 1_2079del;2442C>T
26 human 64210 MMS19 MMS19 homolog, cytosolic ir... NM_001289403.1 29.6% 29.6% 1_2082del;2445C>T
27 human 64210 MMS19 MMS19 homolog, cytosolic ir... XM_017016517.2 28.5% 28.5% 1_2199del;2562C>T
28 human 64210 MMS19 MMS19 homolog, cytosolic ir... XM_006717944.4 28.4% 28.4% 1_2208del;2571C>T
29 human 64210 MMS19 MMS19 homolog, cytosolic ir... NM_001289405.1 28.4% 28.4% 1_2211del;2574C>T
30 human 64210 MMS19 MMS19 homolog, cytosolic ir... NM_022362.5 28.4% 28.4% 1_2211del;2574C>T
31 human 64210 MMS19 MMS19 homolog, cytosolic ir... XM_017016516.2 27.4% 27.4% 1_2325del;2688C>T
32 human 64210 MMS19 MMS19 homolog, cytosolic ir... NM_001351356.1 27.3% 27.4% 1_2328del;2691C>T
33 human 64210 MMS19 MMS19 homolog, cytosolic ir... XR_002957006.1 25.8% (many diffs)
34 human 64210 MMS19 MMS19 homolog, cytosolic ir... XR_001747180.2 25.6% (many diffs)
35 human 64210 MMS19 MMS19 homolog, cytosolic ir... XR_001747179.2 24.8% (many diffs)
36 human 64210 MMS19 MMS19 homolog, cytosolic ir... XR_001747178.2 24.7% (many diffs)
37 mouse 72199 Mms19 MMS19 (MET18 S. cerevisiae) XM_011247354.2 37.6% 40.6% (many diffs)
38 mouse 72199 Mms19 MMS19 (MET18 S. cerevisiae) XM_017318295.1 37.6% 40.6% (many diffs)
39 mouse 72199 Mms19 MMS19 (MET18 S. cerevisiae) XM_011247352.2 37.5% 40.5% (many diffs)
40 mouse 72199 Mms19 MMS19 (MET18 S. cerevisiae) XM_011247353.2 37.5% 40.5% (many diffs)
41 mouse 72199 Mms19 MMS19 (MET18 S. cerevisiae) XM_017318293.1 37.5% 40.5% (many diffs)
42 mouse 72199 Mms19 MMS19 (MET18 S. cerevisiae) XM_017318294.1 37.5% 40.5% (many diffs)
43 mouse 72199 Mms19 MMS19 (MET18 S. cerevisiae) XM_011247350.2 28.5% 30.7% (many diffs)
44 mouse 72199 Mms19 MMS19 (MET18 S. cerevisiae) XM_006527358.3 24.2% 26.2% (many diffs)
45 mouse 72199 Mms19 MMS19 (MET18 S. cerevisiae) NM_028152.3 24.2% 26.1% (many diffs)
46 mouse 72199 Mms19 MMS19 (MET18 S. cerevisiae) XR_001782619.1 21.3% (many diffs)
47 mouse 72199 Mms19 MMS19 (MET18 S. cerevisiae) XR_001782618.1 21.3% (many diffs)
48 mouse 72199 Mms19 MMS19 (MET18 S. cerevisiae) XR_001782617.1 19.8% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
945
ORF length:
879
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcg ggagcttttg gaactgagct gctgccacag ctgccccttt tcttccaccg
121ctgctgccaa gtgctttgca ggactcctca acaagcaccc tgcagggcag cagctggatg
181aattcctaca gctagctgtg gacaaagtgg aggctggcct gggctctggg ccctgtcgta
241gtcaggcctt cactcttctt ctctgggtaa caaaggccct agtgctcaga taccatcctc
301tcagctcctg ccttacagcc cggctcatgg gcctcctgag tgacccagaa ttaggtccag
361cagcagctga tggcttctct ctgctcatgt ctgactgcac tgatgtgctg actcgtgctg
421gccatgctga agtgcggatc atgttccgcc agcggttctt cacagataat gtgcctgctt
481tggtccaggg cttccatgct gctccccaag atgtgaagcc aaactacttg aagggtcttt
541ctcatgtact taacaggctg cccaagcctg tactcttgcc agagctgccc acgcttcttt
601ccttgctgct ggaggccctg tcctgccctg actgtgtggt gcagctctcc accctcagct
661gccttcagcc tcttctactg gaagcacccc aagtcatgag tcttcacgtg gacaccctcg
721tcaccaagtt tctgaacctc agctctagcc cttccatggc tgtccggatc gccgcactgc
781agtgcatgca tgctctcact cgcctgccca cccctgtgct gctgccgtac aaaccacagg
841tgattcgggc cttagccaaa cccctggatg acaagaagag actggtgcgc aaggaagcag
901tgtcagccag aggggagtgg tttctgttgg ggagccctgg cagctgccca accttcttgt
961acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
1021gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

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