Construct: ORF ccsbBroad304_12461
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016650.1_s304c1, BRDN0000400434
- Derived from:
- ccsbBroadEn_12461
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ROBO3 (64221)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12461
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64221 | ROBO3 | roundabout guidance receptor 3 | NM_001370364.1 | 39.7% | 39.7% | 1_663del;1045_1050delCGGAGT |
| 2 | human | 64221 | ROBO3 | roundabout guidance receptor 3 | NM_001370366.1 | 39.7% | 39.7% | 1_663del;1045_1050delCGGAGT |
| 3 | human | 64221 | ROBO3 | roundabout guidance receptor 3 | NR_163415.1 | 38.3% | 1_480del;862_867delCGGAGT;928_1151del | |
| 4 | human | 64221 | ROBO3 | roundabout guidance receptor 3 | NM_001370356.1 | 33.7% | 33.7% | 1_858del;1240_1245delCGGAGT |
| 5 | human | 64221 | ROBO3 | roundabout guidance receptor 3 | NM_001370357.1 | 33.7% | 33.7% | 1_858del;1240_1245delCGGAGT |
| 6 | human | 64221 | ROBO3 | roundabout guidance receptor 3 | NM_001370358.1 | 33.7% | 33.7% | 1_858del;1240_1245delCGGAGT |
| 7 | human | 64221 | ROBO3 | roundabout guidance receptor 3 | NM_001370359.1 | 33.7% | 33.7% | 1_858del;1240_1245delCGGAGT |
| 8 | human | 64221 | ROBO3 | roundabout guidance receptor 3 | NM_001370361.1 | 33.7% | 33.7% | 1_858del;1240_1245delCGGAGT |
| 9 | human | 64221 | ROBO3 | roundabout guidance receptor 3 | NR_163413.1 | 32.7% | 1_675del;1057_1062delCGGAGT;1123_1346del | |
| 10 | human | 64221 | ROBO3 | roundabout guidance receptor 3 | NR_163409.1 | 28.8% | 1_859del;1241_1246delCGGAGT;1307_1530del | |
| 11 | human | 64221 | ROBO3 | roundabout guidance receptor 3 | NR_163414.1 | 27.6% | 1_926del;1308_1313delCGGAGT;1374_1597del | |
| 12 | human | 64221 | ROBO3 | roundabout guidance receptor 3 | NR_163410.1 | 27.2% | 1_950del;1332_1337delCGGAGT;1398_1621del | |
| 13 | human | 64221 | ROBO3 | roundabout guidance receptor 3 | NR_163411.1 | 24.8% | 1_1102del;1484_1489delCGGAGT;1550_1773del | |
| 14 | human | 64221 | ROBO3 | roundabout guidance receptor 3 | NR_163412.1 | 24.2% | 1_1145del;1527_1532delCGGAGT;1593_1816del | |
| 15 | human | 64221 | ROBO3 | roundabout guidance receptor 3 | XM_017018122.1 | 10.7% | 10.7% | 1_3645del;4027_4032delCGGAGT |
| 16 | human | 64221 | ROBO3 | roundabout guidance receptor 3 | NM_022370.4 | 10.6% | 10.6% | 1_3711del;4093_4098delCGGAGT |
| 17 | mouse | 19649 | Robo3 | roundabout guidance receptor 3 | XM_011242426.2 | 14.5% | 14.4% | (many diffs) |
| 18 | mouse | 19649 | Robo3 | roundabout guidance receptor 3 | XM_011242427.2 | 14.5% | 14.4% | (many diffs) |
| 19 | mouse | 19649 | Robo3 | roundabout guidance receptor 3 | XM_011242425.2 | 9.5% | 9.4% | (many diffs) |
| 20 | mouse | 19649 | Robo3 | roundabout guidance receptor 3 | NM_001164767.1 | 9.2% | 9.2% | (many diffs) |
| 21 | mouse | 19649 | Robo3 | roundabout guidance receptor 3 | XM_011242424.2 | 9.2% | 9.2% | (many diffs) |
| 22 | mouse | 19649 | Robo3 | roundabout guidance receptor 3 | XM_006510107.3 | 9.2% | 9.1% | (many diffs) |
| 23 | mouse | 19649 | Robo3 | roundabout guidance receptor 3 | XR_001778823.1 | 7.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 507
- ORF length:
- 441
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac tcccccactt caaggacccc gtgctcgatt ccggaagaaa cccaaggctc 121 ttccctacag gagggagaac agtcctgggg acttgccccc accacccttg ccaccgccag 181 aggaagaggc gagctgggcc ctagagctga gggcagcagg cagcatgtcc tccctggagc 241 gggagcgcag tggggagagg aaagcggtcc aggccgtgcc cctggcagcc cagcgggtgc 301 tccacccaga tgaagaggcc tggctcccat acagcagacc aagcttcctg tcccggggcc 361 agggcaccag cacatgttcc acggccggca gcaactcttc caggggctcc agcagctcta 421 ggggctcccg gggccctggc cggagccgga gtcagagccg gagccagagc caaaggccag 481 gacagaaacg ccgagaggaa ccaagatgcc caactttctt gtacaaagtg gttggtaagc 541 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 601 ggcgcgttaa gtcgacaatc aacctctgga tta