Construct: ORF ccsbBroad304_12489
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004453.1_s304c1, BRDN0000398674
- Derived from:
- ccsbBroadEn_12489
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FNDC3B (64778)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12489
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64778 | FNDC3B | fibronectin type III domain... | XM_017007064.2 | 9.3% | 8.9% | (many diffs) |
| 2 | human | 64778 | FNDC3B | fibronectin type III domain... | XM_017007062.1 | 5.6% | 5.4% | (many diffs) |
| 3 | human | 64778 | FNDC3B | fibronectin type III domain... | NM_001135095.2 | 5.2% | 4.9% | (many diffs) |
| 4 | human | 64778 | FNDC3B | fibronectin type III domain... | NM_022763.4 | 5.2% | 4.9% | (many diffs) |
| 5 | human | 64778 | FNDC3B | fibronectin type III domain... | XM_024453716.1 | 5.2% | 4.9% | (many diffs) |
| 6 | human | 64778 | FNDC3B | fibronectin type III domain... | XM_024453717.1 | 5.2% | 4.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 267
- ORF length:
- 201
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgat gatgaccgac caaatccctc tggaactgcc accattgctg aacggagagg 121 tagccatgat gccccacttg gtgaatggag atgcagctca gcaggttatt ctcgttcaag 181 ttaatccagg tgagactttc acaataagag cagaggatgg aacacttcag tgcattcaag 241 atgaagtggt gaagagagcc tgcgattgcc caactttctt gtacaaagtg gttggtaagc 301 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 361 ggcgcgttaa gtcgacaatc aacctctgga tta