Construct: ORF ccsbBroad304_12495
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF012194.1_s304c1, BRDN0000401097
- Derived from:
- ccsbBroadEn_12495
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CEP85 (64793)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12495
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64793 | CEP85 | centrosomal protein 85 | NM_001281518.3 | 100% | 100% | |
| 2 | human | 64793 | CEP85 | centrosomal protein 85 | NM_001281517.3 | 27.2% | 27.2% | 1_1551del |
| 3 | human | 64793 | CEP85 | centrosomal protein 85 | NM_022778.5 | 25.4% | 25.4% | 1_1704del |
| 4 | human | 64793 | CEP85 | centrosomal protein 85 | XM_024449198.1 | 25.4% | 25.4% | 1_1704del |
| 5 | human | 64793 | CEP85 | centrosomal protein 85 | XM_024449200.1 | 25.4% | 25.4% | 1_1704del |
| 6 | human | 64793 | CEP85 | centrosomal protein 85 | NM_001319944.2 | 25.3% | 25.3% | 1_1704del;2128_2129insCAG |
| 7 | mouse | 70012 | Cep85 | centrosomal protein 85 | XM_006539164.1 | 24.3% | 23.6% | (many diffs) |
| 8 | mouse | 70012 | Cep85 | centrosomal protein 85 | XM_011250331.2 | 23.9% | 23.2% | (many diffs) |
| 9 | mouse | 70012 | Cep85 | centrosomal protein 85 | NM_144527.3 | 22.3% | 21.6% | (many diffs) |
| 10 | mouse | 70012 | Cep85 | centrosomal protein 85 | XM_011250330.2 | 22.3% | 21.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 648
- ORF length:
- 582
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaatag 61 ttggcatgga gtcctggcag aagcgatacg attcgctcca aaagattgtg gagaagcagc 121 agcagaagat ggatcagttg cgctcacaag tacagagcct agagcaggaa gtggctcaag 181 aagaaggaac aagccaggcc ctgagagagg aggcccagcg aagggattca gccctgcagc 241 agctgcgcac agccgtgaag gagctttcag tgcaaaacca ggacttgatt gagaagaatc 301 tgacactcca ggaacacctg cgccaggccc aaccagggtc tccaccttca ccagacacgg 361 cccagctggc acttgagctg caccaggagt tggccagttg ccttcaagat ctgcaggctg 421 tctgtagcat tgtgacccag agggcccagg gccatgaccc caatctctcc ctgctcctgg 481 gcattcactc agcacagcac ccagagactc agctagattt gcagaagcca gatgtgatca 541 agaggaaact agaagaggtt caacagctgc gtcgtgacat tgaggactta aggaccacca 601 tgtcagacag atatgcccag gacatgggag aaaactgtgt cacacagtgc ccaactttct 661 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 721 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta