Construct: ORF ccsbBroad304_12499
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004221.1_s304c1, BRDN0000462025
- Derived from:
- ccsbBroadEn_12499
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SPATA20 (64847)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12499
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 64847 | SPATA20 | spermatogenesis associated 20 | NM_001258373.1 | 99.8% | 99.5% | (many diffs) |
| 2 | human | 64847 | SPATA20 | spermatogenesis associated 20 | NM_001258372.1 | 94.2% | 94% | (many diffs) |
| 3 | human | 64847 | SPATA20 | spermatogenesis associated 20 | NM_022827.4 | 92.3% | 92.1% | (many diffs) |
| 4 | mouse | 217116 | Spata20 | spermatogenesis associated 20 | NM_144827.4 | 79.3% | 83% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2292
- ORF length:
- 2226
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc tgctggaggg aaaggaagcc atccttcatc tacaccccag agggtcccca 121 accgcctgat ccacgagaag tcaccatacc tcctacaaca tgcctacaat cctgtggact 181 ggtacccctg gggagaggaa gccttcgaca aggccaggaa ggaaaacaag ccgattttcc 241 tctcagtcgg gtactccacc tgccactggt gccacatgat ggaagaggag tccttccaga 301 atgaggagat tggccgcctg ctcagtgagg actttgtgag tgtgaaggta gaccgtgagg 361 agcggcctga cgtggacaag gtgtacatga cgttcgtgca ggccaccagc agcggcgggg 421 gctggcccat gaatgtgtgg ctgactccca acctccagcc ctttgtcggg ggcacctatt 481 tccctcctga ggatggcttg acccgagtcg gcttccgcac agtgttgctg agaatacgag 541 aacagtggaa acagaacaag aacaccctgc tagaaaatag ccagcgtgtc accactgccc 601 tgctggcccg atcagagatc agcgtgggtg accgccagct gccgccctct gccgccaccg 661 tgaacaatcg ctgcttccag cagctggatg agggctatga tgaggaatac ggtggcttcg 721 ctgaggcccc caagtttccc acgccggtga tcctgagctt cctgttctcc tactggctca 781 gccatcgact gactcaggat ggctctcggg cccagcagat ggccttgcat accctgaaaa 841 tgatggctaa cgggggcatc cgggaccatg tggggcaggg ctttcaccgc tactccacag 901 accgccagtg gcacgtccct cactttgaga agatgctcta tgaccaggca cagctcgctg 961 tggcctattc gcaggccttc cagctctctg gtgatgaatt ctactctgac gtggccaaag 1021 gcatcctgca gtacgtggct cggagcctga gccaccggtc cggaggcttc tatagcgcag 1081 aggatgcaga ctcgccccca gagcggggcc agcggcccaa agagggcgcc tactatgtgt 1141 ggacggtcaa agaggttcag cagctcctcc cggagcctgt gttgggtgcc accgagccgc 1201 tgacctcagg ccagctcctc atgaagcact acggcctcac agaggctggt aacatcagcc 1261 ccagtcagga ccccaagggg gagctgcagg gccagaatgt gctgaccgtc cggtactcgc 1321 tggagctgac tgctgcccgc tttggcttgg atgtggaggc cgtgcggacc ttgctcaata 1381 cagggctgga gaagctcttc caggcccgga agcatcggcc caagccgcac ctggacagca 1441 agatgctggc tgcctggaat ggcttgatgg tgtcaggcta tgctgtgact ggggctgtcc 1501 tgggccaaga caggctgatc aactatgcca ccaatggtgc caagttcctg aagcggcaca 1561 tgtttgatgt ggccagtggc cgcctgatgc ggacctgcta caccggccct ggggggactg 1621 tggagcacag caacccaccc tgctggggct tcctggagga ctacgccttc gtggtgcggg 1681 gcctgctgga cctgtatgag gcctcacagg agagtgcgtg gctcgagtgg gctctgcggc 1741 tgcaggacac acaggacagg ctcttttggg actcccaggg tggcggctac ttctgcagtg 1801 aggctgagct gggggctggc ctgcccctgc gtctgaagga cgaccaggat ggagcagagc 1861 ccagcgccaa ttccgtgtca gcccacaacc tgctccggct gcatggcttc acgggccaca 1921 aggactggat ggacaagtgt gtgtgcctat tgaccgcctt ttccgagcgc atgcgtcgtg 1981 tcccggtggc gttgcccgag atggtccgcg ccctctcagc ccagcagcag accctcaagc 2041 agatcgtgat ctgtggagac cgtcaggcca aggacaccaa ggccctggtg cagtgcgtcc 2101 actctgtcta cattcctaac aaggtgctga ttctggctga tggggacccc tcgagcttcc 2161 tgtcccgcca gctgcctttc ctgagtaccc tccgacggtt ggaagaccag gccactgcat 2221 atgtgtgtga gaatcaagcc tgctcagtgc ccatcactga tccctgcgaa ttacgaaaac 2281 tactacatcc atgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 2341 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 2401 caatcaacct ctggatta