Construct: ORF ccsbBroad304_12646
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001404.1_s304c1, BRDN0000462409
- Derived from:
- ccsbBroadEn_12646
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CNTNAP3 (79937)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12646
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79937 | CNTNAP3 | contactin associated protei... | NM_033655.3 | 93.5% | 93.5% | (many diffs) |
| 2 | human | 728577 | CNTNAP3B | contactin associated protei... | NM_001201380.2 | 92.4% | 92% | (many diffs) |
| 3 | human | 643827 | CNTNAP3P2 | CNTNAP3 pseudogene 2 | NR_111893.2 | 46.7% | (many diffs) | |
| 4 | human | 100289279 | CNTNAP3C | contactin associated protei... | XM_017015380.1 | 41.6% | 41.2% | (many diffs) |
| 5 | human | 100289279 | CNTNAP3C | contactin associated protei... | XM_011545672.3 | 40.8% | 40.7% | (many diffs) |
| 6 | human | 100289279 | CNTNAP3C | contactin associated protei... | XM_011545673.2 | 30.7% | 29.6% | (many diffs) |
| 7 | human | 100289279 | CNTNAP3C | contactin associated protei... | XM_011545674.2 | 29.6% | 29.5% | 1072_1076delGTGCT;1080_1080delTins2547 |
| 8 | human | 100289279 | CNTNAP3C | contactin associated protei... | XR_950635.1 | 28.5% | 1_376del;1301_1302insAGATC;1516_1517ins2476 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 3690
- ORF length:
- 3621
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcttcagtg gcctgggccg tcctcaaggt gctgctgctt ctccccactc 121 agacttggag ccccgtggga gcaggaaatc cacctgactg tgatgcccca ctggcctctg 181 ccttgcctag gtcatccttc agcagctcct cagagctgtc cagcagccac ggcccggggt 241 tttcaaggct taatcgaaga gatggagctg gtggctggac tccacttgtg tcaaataaat 301 accaatggct gcaaattgac cttggagaga gaatggaggt cactgctgtc gccacccaag 361 gaggatatgg gagctctgac tgggtgacca gctacctcct gatgttcagt gatggtggga 421 gaaactggaa gcagtatcgc cgagaagaaa gcatctgggg ttttccagga aacacaaacg 481 cagacagtgt ggtgcactac agactccagc ctccctttga agccaggttc ctgcgctttc 541 tccctttagc ctggaaccct aggggcagga ttgggatgcg gatcgaagtg tacggatgtg 601 catataaatc tgaggtggtt tattttgatg gacaaagtgc tctgctgtat agacttgata 661 aaaaaccttt aaaaccaata agagacgtta tttctttgaa atttaaagcc atgcagagca 721 atggaattct acttcacaga gaaggacaac atggaaatca cattactctg gaattaatta 781 aaggaaagct tgtctttttt cttaattcag gcaatgctaa gctgccttcc actattgctc 841 ctgtgaccct caccctgggc agcctgctgg acgaccagca ctggcattcc gtcctcatcg 901 agctcctcga cacgcaggtc aacttcaccg tggacaaaca cactcatcat ttccaagcaa 961 agggagattc cagttacttg gatcttaatt ttgagatcag ctttggggga attccgacac 1021 ccggaagatc gcgggcattc agacgtaaaa gctttcatgg gtgtttagaa aatctttatt 1081 ataatggagt ggatgttacc gaattagcca agaaacacaa accacagatc ctcatgatgg 1141 gaaatgtgtc cttctcatgt ccacagccac agactgtccc tgtgactttt ctgagctcca 1201 ggagttatct ggctctgcca ggcaactctg gggaggacaa agtgtctgtc acttttcaat 1261 ttcgaacgtg gaacagagca ggacatttgc ttttcggcga acttcgacgt ggttcaggga 1321 gtttcgtcct ctttcttaag gatggcaagc tcaaactgag tctcttccag ccgggacagt 1381 caccaaggaa tgtcacagca ggtgctggat taaacgatgg gcagtggcac tctgtgtcct 1441 tctctgccaa gtggagccat atgaatgtgg tggtggacga tgacacagct gttcagcccc 1501 tggtggctgt gctcattgat tcaggtgaca cctattattt tggaggctgc ctggacaaca 1561 gctctggctc tggatgtaaa agccccctgg gagggtttca gggctgccta aggctcatca 1621 ccattggtga caaagcggtg gatcccatct tagtacagca gggggcgctg gggagtttca 1681 gggacctcca gatagactcc tgcggcatca cagacaggtg cttgcccagc tactgtgagc 1741 atgggggcga gtgttcccag tcgtgggaca ccttctcctg tgactgtcta ggcacaggct 1801 atacgggcga gacctgccat tcctctctct acgagcagtc ttgtgaagcc cacaagcacc 1861 gagggaaccc gtctgggctt tactatattg atgcagatgg aagtggcccc ctgggaccat 1921 ttcttgtgta ctgcaatatg acagacgccg cgtggacggt ggtgcagcac ggtggccccg 1981 acgcggtgac cctccgaggt gcccccagcg ggcacccgcg ctcggctgtg tccttcgcgt 2041 acgcagcggg cgcggggcag ctgcggtccg cggtgaacct ggcggagcgc tgcgagcaga 2101 ggctggctct gcgctgcggg acggcgcggc gcccggactc acgagatgga accccactga 2161 gctggtgggt tggaagaacc aatgaaacac acacctactg gggaggttct ctgcctgatg 2221 ctcaaaagtg tacttgtgga ttagagggga actgcattga ttctcagtat tactgcaact 2281 gtgatgctgg ccgaaatgaa tggactagtg acacaatagt cctttcccaa aaggagcacc 2341 tgccagtcac tcagattgtg atgacagacg caggccgacc acattccgaa gcagcttata 2401 cactggggcc actgctctgc cgcggagatc agtcattctg gaattcagct tccttcaaca 2461 ctgagacttc ataccttcat ttccctgctt tccacggaga actcactgct gacgtgtgct 2521 tcttttttaa gaccacagtt tcctccgggg tgtttatgga gaacctgggg atcacagatt 2581 tcatcaggat tgagctgcgt gctcccacag aagtgacctt ttccttcgat gtggggaatg 2641 gaccttgtga ggtcacggtg cagtcaccca ctccctttaa tgacaatcag tggcaccacg 2701 tgagggcaga gagaaatgtt aaaggagcgt ctcttcaagt tgatcagctt cctcagaaga 2761 tgcagcctgc ccctgctgat gggcacgttc gtttacagct caacagccag ctcttcattg 2821 agatttccgc atattttgca actggctcct caatgacata ccattttcaa gaacattaca 2881 ctttaagtga aaactccagc tctctcgttt cttcattaca cagagatgta acattgacca 2941 gagaaatgat cacactgagc ttccgaacca cacgaactcc gagcttattg ctgtatgtga 3001 gctctttcta tgaggaatac ctttcagtta tcctcgccaa caatggaagt ttgcagatta 3061 ggtacaagct agatagacat caaaatcctg atgcatttac ctttgatttt aaaaacatgg 3121 ctgatgggca acttcaccaa gtgaagatta acagagaaga agctgtggtc atggtagagg 3181 ttaaccagag cgcaaagaaa caagtcatct tgtcctcagg gacagaattc aacgctgtca 3241 aatctctcat attgggaaag gttttagagg ctgccggcgc ggacccggac acaaggcggg 3301 cggcgactag tggcttcacc ggctgcctct cggcggtgcg cttcggccgc gctgctcccc 3361 tgaaggcggc gctgcgcccc agcggcccct cccgggtcac cgtccgcggc cacgtggccc 3421 ctatggcccg ctgcgcagcg ggggcggcgt ccggctcccc ggcgcgggaa ctggctcccc 3481 gactcgcggg gggcgcaggt cgttctggac cagcggatga gggagagccc ttggttaatg 3541 cagacagaag agactctgct gtcatcggag gtgtgatagc agtggtgata tttattttgc 3601 tttgcatcac tgccatagcc atacgcatct atcaacagag aaagttacgc aaagaaaatg 3661 agtcaaaagt ctcaaaaaaa gaagagtgct tgccaacttt cttgtacaaa gtggttggta 3721 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 3781 ggtggcgcgt taagtcgaca atcaacctct ggatta