Construct: ORF ccsbBroad304_12651

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008782.1_s304c1, BRDN0000400058
Derived from:
ccsbBroadEn_12651
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
WLS (79971)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12651
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79971 WLS Wnt ligand secretion mediator NM_001193334.1 93.8% 93.3% (many diffs)
2 human 79971 WLS Wnt ligand secretion mediator XM_017002390.2 88.8% 88.9% 1_165del;297G>A
3 human 79971 WLS Wnt ligand secretion mediator XM_011542192.3 82.3% 80.3% (many diffs)
4 human 79971 WLS Wnt ligand secretion mediator NM_024911.7 81.4% 81.5% 1_300del;432G>A
5 human 79971 WLS Wnt ligand secretion mediator NM_001002292.3 75.9% 74.1% (many diffs)
6 human 79971 WLS Wnt ligand secretion mediator XM_011542191.2 75.6% 73.9% (many diffs)
7 mouse 68151 Wls wntless homolog (Drosophila) NM_026582.4 73.6% 79.8% (many diffs)
8 mouse 68151 Wls wntless homolog (Drosophila) XM_006501964.2 73.6% 79.8% (many diffs)
9 mouse 68151 Wls wntless homolog (Drosophila) NR_037590.1 43% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1389
ORF length:
1323
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga gatgagtcct tggttccaat tcatgctgtt tatcctgcag ctggacattg
121ccttcaagct aaacaaccaa atcagagaaa atgcagaagt ctccatggac gtttccctgg
181cttaccgtga tgacgcattt gctgagtgga ctgaaatggc ccatgaaaga gtaccacgga
241aactcaaatg caccttcaca tctcccaaga ctccagagca tgagggccgt tactatgaat
301gtgatgtcct tcctttcatg gaaattgggt ctgtggccca taagttttac cttttaaaca
361tccggctgcc tgtgaatgag aagaagaaaa tcaatgtggg aattggggag ataaaggata
421tccggttggt ggggatccac caaaatggag gcttcaccaa ggtgtggttt gccatgaaga
481ccttccttac gcccagcatc ttcatcatta tggtgtggta ttggaggagg atcaccatga
541tgtcccgacc cccagtgctt ctggaaaaag tcatctttgc ccttgggatt tccatgacct
601ttatcaatat cccagtggaa tggttttcca tcgggtttga ctggacctgg atgctgctgt
661ttggtgacat ccgacagggc atcttctatg cgatgcttct gtccttctgg atcatcttct
721gtggcgagca catgatggat cagcacgagc ggaaccacat cgcagggtat tggaagcaag
781tcggacccat tgccgttggc tccttctgcc tcttcatatt tgacatgtgt gagagagggg
841tacaactcac gaatcccttc tacagtatct ggactacaga cattggaaca gagctggcca
901tggccttcat catcgtggct ggaatctgcc tctgcctcta cttcctgttt ctatgcttca
961tggtatttca ggtgtttcgg aacatcagtg ggaagcagtc cagcctgcca gctatgagca
1021aagtccggcg gctacactat gaggggctaa tttttaggtt caagttcctc atgcttatca
1081ccttggcctg cgctgccatg actgtcatct tcttcatcgt tagtcaggta acggaaggcc
1141attggaaatg gggcggcgtc acagtccaag tgaacagtgc ctttttcaca ggcatctatg
1201ggatgtggaa tctgtatgtc tttgctctga tgttcttgta tgcaccatcc cataaaaact
1261atggagaaga ccagtccaat ggcgatctgg gtgtccatag tggggaagaa ctccagctca
1321ccaccactat cacccatgtg gacggaccca ctgagatcta caagttgacc cgcaaggagg
1381cccaggagta cctaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc
1441tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa
1501tcaacctctg gatta

Download FASTA (ORF) (Full)