Construct: ORF ccsbBroad304_12664
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000768.1_s304c1, BRDN0000399747
- Derived from:
- ccsbBroadEn_12664
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FBXL18 (80028)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12664
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80028 | FBXL18 | F-box and leucine rich repe... | NM_001367780.1 | 41.9% | 10.4% | 1_1020del;1081_1082delAG;1800_1854del |
| 2 | human | 80028 | FBXL18 | F-box and leucine rich repe... | NM_001367781.1 | 41.9% | 10.4% | 1_1020del;1081_1082delAG;1800_1854del |
| 3 | human | 80028 | FBXL18 | F-box and leucine rich repe... | NM_024963.6 | 36% | 9% | 1_1320del;1381_1382delAG;2100_2154del |
| 4 | human | 80028 | FBXL18 | F-box and leucine rich repe... | NM_001363442.2 | 34% | 8.7% | (many diffs) |
| 5 | human | 80028 | FBXL18 | F-box and leucine rich repe... | NM_001363441.2 | 33.5% | 9.4% | (many diffs) |
| 6 | human | 80028 | FBXL18 | F-box and leucine rich repe... | NM_001321213.2 | 30% | 9.1% | (many diffs) |
| 7 | mouse | 231863 | Fbxl18 | F-box and leucine-rich repe... | NM_001033312.3 | 31.9% | 10.9% | (many diffs) |
| 8 | mouse | 231863 | Fbxl18 | F-box and leucine-rich repe... | XM_006504693.3 | 29.2% | 10.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 843
- ORF length:
- 777
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca cgcagtgccg cgcggctttg gcaagaaagt gcgtgtgggc gtgcagtcct 121 gtccccccct tctcgggcca ggcgtgcccc cagccctcct ccgtgttctg gtctctgctg 181 aagaacctgc ccttcctgga acacctcgag ctgattgggt ccaacttctc ctccgccatg 241 ccccgcaacg agcccgccat ccgcaactcg ctcccaccct gcagccgcgc acagagtgtc 301 ggggactcgg aggtggccgc catcggccag ctggccttcc tgcggcacct gacgctcgca 361 cagctgccca gcgtccttac gggctccggg ctggtcaata tcggcctgca gtgccagcag 421 ttgcggtccc tgtcgctggc caacctgggc atgatgggga aggtggtgta catgcccgcg 481 ctctcagaca tgttgaagca ctgcaagcgg ctgagggacc tcaggctgga gcagccctac 541 ttcagcgcca acgcccagtt cttccaggcg ctgagccagt gcccctcgct gcagcgcctg 601 tgcctggtct ctcgcagcgg caccctccag cccgatgccg tgctggcctt catggctcgc 661 tgcctgcagg ttgtcatgtg ccacctgttc accggggagt ccctcgccac ctgcaagagc 721 ctgcagcagt cgcttctccg cagcttccag gccgagcggc ccgcgttaaa cgtcgtcatc 781 ttccctctgc tccacgaggg cctgaccgac gtcatccggg acgtccccct ggtgcacctg 841 gatgcccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 901 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 961 tctggatta