Transcript: Human NM_001363441.2

Homo sapiens F-box and leucine rich repeat protein 18 (FBXL18), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-02-28
Taxon:
Homo sapiens (human)
Gene:
FBXL18 (80028)
Length:
2298
CDS:
136..2223

Additional Resources:

NCBI RefSeq record:
NM_001363441.2
NBCI Gene record:
FBXL18 (80028)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001363441.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000156441 CAGACATGTTGAAGCACTGCA pLKO.1 1877 CDS 100% 2.640 2.112 N FBXL18 n/a
2 TRCN0000431699 CCCTCCTGCAGCACATGAAAT pLKO_005 1052 CDS 100% 13.200 9.240 N FBXL18 n/a
3 TRCN0000431722 AGAACCTGCGGGTCTTCTATG pLKO_005 818 CDS 100% 10.800 7.560 N FBXL18 n/a
4 TRCN0000155770 CAACCCGTTCTACTTCAGTTT pLKO.1 1077 CDS 100% 4.950 3.465 N FBXL18 n/a
5 TRCN0000156770 GTTCTGGTCTCTGCTGAAGAA pLKO.1 1557 CDS 100% 4.950 3.465 N FBXL18 n/a
6 TRCN0000156053 CACAGATCTGATTCTGAACGT pLKO.1 270 CDS 100% 2.640 1.848 N FBXL18 n/a
7 TRCN0000156286 CTGCTGCTCTACTTCGAGATT pLKO.1 724 CDS 100% 4.950 2.970 N FBXL18 n/a
8 TRCN0000156908 GATGATGATGACATGCACCCT pLKO.1 166 CDS 100% 0.660 0.396 N FBXL18 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001363441.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14279 pDONR223 100% 52.3% 52.2% None 1090C>T;1091_1092insT;1094_2085delinsC n/a
2 ccsbBroad304_14279 pLX_304 0% 52.3% 52.2% V5 1090C>T;1091_1092insT;1094_2085delinsC n/a
3 ccsbBroadEn_12664 pDONR223 100% 33.5% 9.4% None (many diffs) n/a
4 ccsbBroad304_12664 pLX_304 0% 33.5% 9.4% V5 (many diffs) n/a
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