Transcript: Human NM_001367781.1

Homo sapiens F-box and leucine rich repeat protein 18 (FBXL18), transcript variant 6, mRNA.

Source:
NCBI, updated 2019-02-28
Taxon:
Homo sapiens (human)
Gene:
FBXL18 (80028)
Length:
8154
CDS:
320..2176

Additional Resources:

NCBI RefSeq record:
NM_001367781.1
NBCI Gene record:
FBXL18 (80028)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001367781.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000416290 CCGCGTTAAACGTCGTCATCT pLKO_005 2037 CDS 100% 4.950 3.960 N FBXL18 n/a
2 TRCN0000156441 CAGACATGTTGAAGCACTGCA pLKO.1 1761 CDS 100% 2.640 2.112 N FBXL18 n/a
3 TRCN0000431699 CCCTCCTGCAGCACATGAAAT pLKO_005 936 CDS 100% 13.200 9.240 N FBXL18 n/a
4 TRCN0000431722 AGAACCTGCGGGTCTTCTATG pLKO_005 702 CDS 100% 10.800 7.560 N FBXL18 n/a
5 TRCN0000155770 CAACCCGTTCTACTTCAGTTT pLKO.1 961 CDS 100% 4.950 3.465 N FBXL18 n/a
6 TRCN0000156770 GTTCTGGTCTCTGCTGAAGAA pLKO.1 1441 CDS 100% 4.950 3.465 N FBXL18 n/a
7 TRCN0000156053 CACAGATCTGATTCTGAACGT pLKO.1 154 5UTR 100% 2.640 1.848 N FBXL18 n/a
8 TRCN0000156286 CTGCTGCTCTACTTCGAGATT pLKO.1 608 CDS 100% 4.950 2.970 N FBXL18 n/a
9 TRCN0000078113 GCCTGTAATCCCAGCACTTTA pLKO.1 6785 3UTR 100% 13.200 6.600 Y LIAS n/a
10 TRCN0000140657 CCTCCCAAAGTGCTAGGATAA pLKO.1 6485 3UTR 100% 10.800 5.400 Y CD3EAP n/a
11 TRCN0000222574 CGCCTGTAATCCCAGCACTTT pLKO.1 6784 3UTR 100% 4.950 2.475 Y ERAP2 n/a
12 TRCN0000165027 GAACTCCTGACCTCAAGTGAT pLKO.1 6451 3UTR 100% 4.950 2.475 Y LOC387873 n/a
13 TRCN0000072628 CCTCCCAAAGTGCTAGGATTA pLKO.1 6485 3UTR 100% 10.800 5.400 Y MRPS16 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001367781.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12664 pDONR223 100% 41.9% 10.4% None 1_1020del;1081_1082delAG;1800_1854del n/a
2 ccsbBroad304_12664 pLX_304 0% 41.9% 10.4% V5 1_1020del;1081_1082delAG;1800_1854del n/a
3 ccsbBroadEn_14279 pDONR223 100% 36.7% 36.6% None (many diffs) n/a
4 ccsbBroad304_14279 pLX_304 0% 36.7% 36.6% V5 (many diffs) n/a
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