Construct: ORF ccsbBroad304_12745
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017401.1_s304c1, BRDN0000410854
- Derived from:
- ccsbBroadEn_12745
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CCM2 (83605)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12745
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83605 | CCM2 | CCM2 scaffold protein | XM_017012671.1 | 21.7% | 18.8% | (many diffs) |
| 2 | human | 83605 | CCM2 | CCM2 scaffold protein | NM_001167935.1 | 20% | 19.2% | (many diffs) |
| 3 | human | 83605 | CCM2 | CCM2 scaffold protein | XM_006715786.3 | 18.5% | 16% | (many diffs) |
| 4 | human | 83605 | CCM2 | CCM2 scaffold protein | NM_031443.3 | 15.9% | 15.3% | (many diffs) |
| 5 | human | 83605 | CCM2 | CCM2 scaffold protein | NM_001029835.2 | 14.8% | 12.9% | (many diffs) |
| 6 | human | 83605 | CCM2 | CCM2 scaffold protein | NM_001363458.2 | 14.5% | 14% | (many diffs) |
| 7 | human | 83605 | CCM2 | CCM2 scaffold protein | XM_011515561.2 | 13.6% | 11.9% | (many diffs) |
| 8 | human | 83605 | CCM2 | CCM2 scaffold protein | XR_428088.3 | 11.7% | (many diffs) | |
| 9 | human | 83605 | CCM2 | CCM2 scaffold protein | NR_030770.2 | 11.1% | (many diffs) | |
| 10 | mouse | 216527 | Ccm2 | cerebral cavernous malforma... | NM_146014.3 | 15% | 14.7% | (many diffs) |
| 11 | mouse | 216527 | Ccm2 | cerebral cavernous malforma... | XM_006514622.1 | 13.7% | 13.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 279
- ORF length:
- 213
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga agaggagggc aagaagggca agaagcctgg aattgtctcg ccatttaaac 121 gagtattcct aaaaggtgaa aagagtagag ataagaaagc ccatgagaag gtgacagaga 181 ggcgccctct gcacactgtg gtgttgtcat tgcctgagcg cgtcgagcca gacagactgc 241 tgagcgacta tattgagaag gaggtaaagg gctcccatta cccaactttc ttgtacaaag 301 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 361 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta