Construct: ORF ccsbBroad304_12764

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF014604.1_s304c1, BRDN0000402633
• Derived from: ccsbBroadEn_12764
• DNA Barcode: None
• Epitope Tag: V5
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: IMMP2L (83943)

Vector Information:

• Vector Backbone: pLX_304
• Pol II Cassette 1: PGK-BlastR
• Pol II Cassette 2: CMV-ccsbBroad304_12764
• Selection Marker: BlastR
• Visible Reporter: n/a
• Epitope Tag: V5

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	83943	IMMP2L	inner mitochondrial membran...	NM_001350962.2	100%	100%
2	human	83943	IMMP2L	inner mitochondrial membran...	NM_001350963.2	90.5%	92.7%	306_312delGTTTCCCins25
3	human	83943	IMMP2L	inner mitochondrial membran...	XM_024446958.1	84.9%	67.7%	(many diffs)
4	human	83943	IMMP2L	inner mitochondrial membran...	XM_024446957.1	79.9%	72.1%	(many diffs)
5	human	83943	IMMP2L	inner mitochondrial membran...	XM_024446960.1	79%	71.6%	(many diffs)
6	human	83943	IMMP2L	inner mitochondrial membran...	XM_024446959.1	78.7%	73.2%	(many diffs)
7	human	83943	IMMP2L	inner mitochondrial membran...	XM_011516611.2	78.3%	67.7%	(many diffs)
8	human	83943	IMMP2L	inner mitochondrial membran...	XM_024446961.1	77.2%	73.6%	(many diffs)
9	human	83943	IMMP2L	inner mitochondrial membran...	XM_024446962.1	73.7%	72.7%	239delT;241T>G;246_247ins85
10	human	83943	IMMP2L	inner mitochondrial membran...	NM_001350964.2	73.6%	72.7%	(many diffs)
11	human	83943	IMMP2L	inner mitochondrial membran...	XM_011516609.2	72.4%	73.9%	240_323del;390_396delGTTTCCCins25
12	human	83943	IMMP2L	inner mitochondrial membran...	NM_001244606.2	60.6%	59.4%	(many diffs)
13	human	83943	IMMP2L	inner mitochondrial membran...	NM_001350959.2	60.6%	59.4%	(many diffs)
14	human	83943	IMMP2L	inner mitochondrial membran...	NM_001350960.2	60.6%	59.4%	(many diffs)
15	human	83943	IMMP2L	inner mitochondrial membran...	NM_032549.4	60.6%	59.4%	(many diffs)
16	human	83943	IMMP2L	inner mitochondrial membran...	XM_011516608.2	60.2%	59%	(many diffs)
17	human	83943	IMMP2L	inner mitochondrial membran...	XM_017012704.1	60.2%	59%	(many diffs)
18	human	83943	IMMP2L	inner mitochondrial membran...	XM_017012703.1	57.6%	56.5%	(many diffs)
19	human	83943	IMMP2L	inner mitochondrial membran...	NM_001350961.2	52.2%	51.2%	(many diffs)
20	human	83943	IMMP2L	inner mitochondrial membran...	XM_017012701.1	44.2%	43.3%	(many diffs)
21	human	83943	IMMP2L	inner mitochondrial membran...	XM_017012702.1	44.2%	43.3%	(many diffs)
22	human	83943	IMMP2L	inner mitochondrial membran...	XM_017012699.1	39.6%	38.8%	(many diffs)
23	human	83943	IMMP2L	inner mitochondrial membran...	XM_017012700.1	39.6%	38.8%	(many diffs)
24	human	83943	IMMP2L	inner mitochondrial membran...	XM_024446956.1	36.5%	33.7%	(many diffs)
25	mouse	93757	Immp2l	IMP2 inner mitochondrial me...	NM_053122.4	54.6%	55.4%	(many diffs)

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

66

ORF end:

396

ORF length:

330

Sequence:

1	ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61	ttggcatggc acagtcacaa gggtgggtga aaagatacat caaggccttt tgtaaaggct
121	tctttgtggc ggtgcctgtg gcagtgactt tcttggatcg ggtcgcctgt gtggcaagag
181	tagaaggagc atcgatgcag ccttctttga atcctggggg gagccagtca tctgatgtgg
241	tgcttttgaa ccactggaaa gtgaggaatt ttgaagtaca ccgtggtgac attgtatcat
301	tggtgtctcc taaaaaccca gaacagaaga tcattaagag agtgattgct cttgaaggag
361	atattgtcag agatgggaga aaactgaaaa ggatatgccc aactttcttg tacaaagtgg
421	ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg
481	ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta

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