Construct: ORF ccsbBroad304_12850
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004446.1_s304c1, BRDN0000407298
- Derived from:
- ccsbBroadEn_12850
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MGC10997 (84741)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12850
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 104472716 | WARS2-IT1 | WARS2 intronic transcript 1 | NR_126447.1 | 19.7% | (many diffs) | |
| 2 | human | 400121 | LINC00547 | long intergenic non-protein... | NR_040244.1 | 3.3% | (many diffs) | |
| 3 | human | 101928808 | LOC101928808 | uncharacterized LOC101928808 | XR_244519.1 | 2.8% | (many diffs) | |
| 4 | human | 101929048 | LOC101929048 | uncharacterized LOC101929048 | XR_001741387.1 | 2.3% | (many diffs) | |
| 5 | human | 283487 | LINC00346 | long intergenic non-protein... | NR_027701.1 | 1.8% | (many diffs) | |
| 6 | human | 162655 | ZNF519 | zinc finger protein 519 | NR_033354.2 | 1.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 192
- ORF length:
- 126
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg ggaaagtttg gaacttccta gagacttgtt gaatggcttt gaccaaaatg 121 ctgataatga tgtggtcaat gaaatccagg ctgaggtggt ctcagatgga gatgaggaac 181 ttgttgggaa ctgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 241 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 301 caatcaacct ctggatta