Transcript: Human NR_027701.1

Homo sapiens long intergenic non-protein coding RNA 346 (LINC00346), long non-coding RNA.

Source:
NCBI, updated 2019-06-11
Taxon:
Homo sapiens (human)
Gene:
LINC00346 (283487)
Length:
6322
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
NR_027701.1
NBCI Gene record:
LINC00346 (283487)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NR_027701.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000164025 CTTACGAGGCATGTTTCCTTT pLKO.1 648 3UTR 100% 4.950 6.930 N LINC00346 n/a
2 TRCN0000161598 GACATTCATGGACTTTCGTTT pLKO.1 825 3UTR 100% 4.950 6.930 N LINC00346 n/a
3 TRCN0000166749 CTGTCCTTACGAGGCATGTTT pLKO.1 643 3UTR 100% 5.625 3.938 N LINC00346 n/a
4 TRCN0000165709 CAAGCGGAAGGAAGTGTGTTT pLKO.1 740 3UTR 100% 4.950 3.465 N LINC00346 n/a
5 TRCN0000166511 CCTTACGAGGCATGTTTCCTT pLKO.1 647 3UTR 100% 3.000 2.100 N LINC00346 n/a
6 TRCN0000162407 CCCATTGTATCTTGGAAGTAA pLKO.1 5404 3UTR 100% 5.625 2.813 Y LINC00663 n/a
7 TRCN0000165027 GAACTCCTGACCTCAAGTGAT pLKO.1 1849 3UTR 100% 4.950 2.475 Y LOC387873 n/a
8 TRCN0000165833 GCTCACTACAACCTCCACTTT pLKO.1 1699 3UTR 100% 4.950 2.475 Y LINC00346 n/a
9 TRCN0000155836 CCCAAAGTGCTGGGATTACAA pLKO.1 1885 3UTR 100% 5.625 2.813 Y KLHL30 n/a
10 TRCN0000141025 CCCAAAGTGCTGGGATTACTT pLKO.1 1885 3UTR 100% 5.625 2.813 Y EID2B n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NR_027701.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_10594 pDONR223 100% 7.7% None 1_518del;1011_6322del n/a
2 ccsbBroad304_10594 pLX_304 0% 7.7% V5 1_518del;1011_6322del n/a
3 TRCN0000476924 CGGTCGGATAGCCTTCGCACGAAA pLX_317 72.4% 7.7% V5 1_518del;1011_6322del n/a
4 ccsbBroadEn_12850 pDONR223 100% 1.8% None (many diffs) n/a
5 ccsbBroad304_12850 pLX_304 0% 1.8% V5 (many diffs) n/a
6 TRCN0000475148 TGCTGTCGGCAGCCCTCCTCTTAC pLX_317 100% 1.8% V5 (many diffs) n/a
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