Construct: ORF ccsbBroadEn_12850
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004446.1_s300c1, BRDN0000393407
- DNA Barcode:
- None
- Epitope Tag:
- None
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MGC10997 (84741)
Vector Information:
- Vector Backbone:
- pDONR223
- Pol II Cassette 1:
- n/a
- Pol II Cassette 2:
- n/a
- Selection Marker:
- n/a
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 104472716 | WARS2-IT1 | WARS2 intronic transcript 1 | NR_126447.1 | 19.7% | (many diffs) | |
| 2 | human | 400121 | LINC00547 | long intergenic non-protein... | NR_040244.1 | 3.3% | (many diffs) | |
| 3 | human | 101928808 | LOC101928808 | uncharacterized LOC101928808 | XR_244519.1 | 2.8% | (many diffs) | |
| 4 | human | 101929048 | LOC101929048 | uncharacterized LOC101929048 | XR_001741387.1 | 2.3% | (many diffs) | |
| 5 | human | 283487 | LINC00346 | long intergenic non-protein... | NR_027701.1 | 1.8% | (many diffs) | |
| 6 | human | 162655 | ZNF519 | zinc finger protein 519 | NR_033354.2 | 1.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 192
- ORF length:
- 126
- Sequence:
-
1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaaCTTTG TACAAAAAAG 61 TTGGCATGGG GGAAAGTTTG GAACTTCCTA GAGACTTGTT GAATGGCTTT GACCAAAATG 121 CTGATAATGA TGTGGTCAAT GAAATCCAGG CTGAGGTGGT CTCAGATGGA GATGAGGAAC 181 TTGTTGGGAA CTGCCCAACT TTCTTGTACA AAGTtggcat tataagaaag cattgcttat 241 caatttgttg caacgaac