Construct: ORF ccsbBroad304_12851

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009803.1_s304c1, BRDN0000400159
Derived from:
ccsbBroadEn_12851
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
USP30 (84749)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12851
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 84749 USP30 ubiquitin specific peptidas... NM_032663.5 98.1% 98.2% 1_27del;639T>C
2 human 84749 USP30 ubiquitin specific peptidas... XM_005253962.3 98% 98% 1_27del;577_578insAGC;636T>C
3 human 84749 USP30 ubiquitin specific peptidas... NM_001301175.1 95.6% 95.6% 0_1ins66;546T>C
4 human 84749 USP30 ubiquitin specific peptidas... XM_006719653.3 95.6% 95.6% 0_1ins66;546T>C
5 human 84749 USP30 ubiquitin specific peptidas... XM_024449227.1 92.3% 92.3% 1_27del;639T>C;947_1045del
6 human 84749 USP30 ubiquitin specific peptidas... XM_017020048.1 91.6% 91.6% 1_27del;639T>C;1168_1278del
7 human 84749 USP30 ubiquitin specific peptidas... XM_017020049.1 91.4% 91.5% (many diffs)
8 human 84749 USP30 ubiquitin specific peptidas... XM_017020050.1 89.1% 89.1% 0_1ins66;546T>C;1075_1185del
9 human 84749 USP30 ubiquitin specific peptidas... XM_017020051.2 89.1% 89.1% 0_1ins66;546T>C;1075_1185del
10 human 84749 USP30 ubiquitin specific peptidas... XM_017020052.2 89.1% 89.1% 0_1ins66;546T>C;1075_1185del
11 human 84749 USP30 ubiquitin specific peptidas... XM_005253965.4 86.3% 86.4% 1_27del;189_190ins183;456T>C
12 human 84749 USP30 ubiquitin specific peptidas... XM_024449228.1 83.5% 83.6% 0_1ins66;96_97ins183;363T>C
13 human 84749 USP30 ubiquitin specific peptidas... XM_017020053.1 80.6% 80.6% (many diffs)
14 human 84749 USP30 ubiquitin specific peptidas... XM_011538894.2 72.3% 72.4% 0_1ins417;133_134insAGC;192T>C
15 human 84749 USP30 ubiquitin specific peptidas... XM_017020054.1 67.4% 67.5% (many diffs)
16 mouse 100756 Usp30 ubiquitin specific peptidas... NM_001033202.3 84.2% 88.2% (many diffs)
17 mouse 100756 Usp30 ubiquitin specific peptidas... XM_017320581.1 84% 88% (many diffs)
18 mouse 100756 Usp30 ubiquitin specific peptidas... XM_011248156.2 62.4% 64.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1590
ORF length:
1524
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgac cgcggccgac agggccatcc agcgcttcct gcggaccggg gcggccgtca
121gatataaagt catgaagaac tggggagtta taggtggaat tgctgctgct cttgcagcag
181gaatatatgt tatttggggt cccattacag aaagaaagaa gcgtagaaaa gggcttgtgc
241ctggccttgt taatttaggg aacacctgct tcatgaactc cctgctacaa ggcctgtctg
301cctgtcctgc tttcatcagg tggctggaag agttcacctc ccagtactcc agggatcaga
361aggagccccc ctcacaccag tatttatcct taacactctt gcaccttctg aaagccttgt
421cctgccaaga agttactgat gatgaggtct tagatgcaag ctgcttgttg gatgtcttaa
481gaatgtacag atggcagatc tcatcatttg aagaacagga tgctcacgaa ttattccatg
541tcattacctc gtcattggaa gatgagcgag accgccagcc tcgggtcaca catttgtttg
601atgtgcattc cctggagcag cagtcagaaa taactcccaa acaaattacc tgccgcacaa
661gagggtcacc tcaccccaca tccaatcact ggaagtctca acatcctttt catggaagac
721tcactagtaa tatggtctgc aaacactgtg aacaccagag tcctgttcga tttgatacct
781ttgatagcct ttcactaagt attccagccg ccacatgggg tcacccattg accctggacc
841actgccttca ccacttcatc tcatcagaat cagtgcggga tgttgtgtgt gacaactgta
901caaagattga agccaaggga acgttgaacg gggaaaaggt ggaacaccag aggaccactt
961ttgttaaaca gttaaaacta gggaagctcc ctcagtgtct ctgcatccac ctacagcggc
1021tgagctggtc cagccacggc acgcctctga agcggcatga gcacgtgcag ttcaatgagt
1081tcctgatgat ggacatttac aagtaccacc tccttggaca taaacctagt caacacaacc
1141ctaaactgaa caagaaccca gggcctacac tggagctgca ggatgggccg ggagccccca
1201caccagttct gaatcagcca ggggccccca aaacacagat ttttatgaat ggcgcctgct
1261ccccatcttt attgccaacg ctgtcagcgc cgatgccctt ccctctccca gttgttcccg
1321actacagctc ctccacatac ctcttccggc tgatggcagt tgtcgtccac catggagaca
1381tgcactctgg acactttgtc acttaccgac ggtccccacc ttctgccagg aaccctctct
1441caactagcaa tcagtggctg tgggtctccg atgacactgt ccgcaaggcc agcctgcagg
1501aggtcctgtc ctccagcgcc tacctgctgt tctacgagcg cgtcctttcc aggatgcagc
1561accagagcca ggagtgcaag tctgaagaat gcccaacttt cttgtacaaa gtggttggta
1621agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc
1681ggtggcgcgt taagtcgaca atcaacctct ggatta

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