Transcript: Mouse XM_017320581.1

PREDICTED: Mus musculus ubiquitin specific peptidase 30 (Usp30), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Usp30 (100756)
Length:
3364
CDS:
92..1642

Additional Resources:

NCBI RefSeq record:
XM_017320581.1
NBCI Gene record:
Usp30 (100756)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_017320581.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000030885 CCTGTTCGGTTTGACACCTTT pLKO.1 812 CDS 100% 4.950 3.960 N Usp30 n/a
2 TRCN0000030884 GCACCTTTGTTAAACAGTTAA pLKO.1 1005 CDS 100% 13.200 9.240 N Usp30 n/a
3 TRCN0000030887 GCAACCAATGGCTGTGGATTT pLKO.1 1497 CDS 100% 10.800 7.560 N Usp30 n/a
4 TRCN0000030886 CCTGATGATGGACTTCTACAA pLKO.1 1132 CDS 100% 4.950 3.465 N Usp30 n/a
5 TRCN0000030888 CACCAGTAACATGGTGTGCAA pLKO.1 772 CDS 100% 0.264 0.185 N Usp30 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017320581.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12851 pDONR223 100% 84% 88% None (many diffs) n/a
2 ccsbBroad304_12851 pLX_304 0% 84% 88% V5 (many diffs) n/a
3 TRCN0000472175 TAAAGTCACCCCCAAATTTCTTAA pLX_317 29.9% 84% 88% V5 (many diffs) n/a
4 TRCN0000487932 TGGAGCCCGATACTTACTTGACAC pLX_317 16.9% 84% 88% V5 (not translated due to prior stop codon) (many diffs) n/a
5 TRCN0000491356 ATCGCTGCCCGGGCTCGGGTGGGT pLX_317 4.3% 83.9% 87.8% V5 (many diffs) n/a
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