Construct: ORF ccsbBroad304_12873

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002539.1_s304c1, BRDN0000402140
Derived from:
ccsbBroadEn_12873
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MIGA2 (84895)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12873
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 84895 MIGA2 mitoguardin 2 XM_011519118.1 47.2% 47.2% 1_717del
2 human 84895 MIGA2 mitoguardin 2 NM_001329990.2 36% 36% 1_1137del
3 human 84895 MIGA2 mitoguardin 2 NM_032809.3 32.5% 32.5% 1_1329del
4 human 84895 MIGA2 mitoguardin 2 XR_929849.2 28.1% 1_1363del;1630_1691del;2068_2277del
5 human 84895 MIGA2 mitoguardin 2 NR_138422.2 17.4% (many diffs)
6 human 84895 MIGA2 mitoguardin 2 XR_929851.2 17.3% 1_1434del;2077_3708del
7 human 84895 MIGA2 mitoguardin 2 XR_001746398.1 17% 1_1481del;2124_3755del
8 human 84895 MIGA2 mitoguardin 2 XR_929850.2 17% 1_1363del;1630_1767del;2144_3775del
9 human 84895 MIGA2 mitoguardin 2 NR_138425.2 15.1% 1_1407del;1674_2240del;2617_4248del
10 human 84895 MIGA2 mitoguardin 2 NR_138424.2 15% 1_1336del;1603_2245del;2622_4253del
11 human 84895 MIGA2 mitoguardin 2 XR_001746392.1 15% 1_1363del;1630_2272del;2649_4280del
12 human 84895 MIGA2 mitoguardin 2 XR_001746395.1 14.7% 1_1434del;1701_2343del;2720_4351del
13 human 84895 MIGA2 mitoguardin 2 NR_138421.2 14.6% 1_1457del;1724_2366del;2743_4374del
14 human 84895 MIGA2 mitoguardin 2 NR_138423.2 14.4% (many diffs)
15 mouse 108958 Miga2 mitoguardin 2 XM_006497595.2 47.3% 50.4% (many diffs)
16 mouse 108958 Miga2 mitoguardin 2 NM_001242407.1 32% 34.2% (many diffs)
17 mouse 108958 Miga2 mitoguardin 2 NM_175392.3 32% 34.2% (many diffs)
18 mouse 108958 Miga2 mitoguardin 2 XM_006497593.2 28% 29.8% (many diffs)
19 mouse 108958 Miga2 mitoguardin 2 XM_006497594.2 22.7% 24.3% (many diffs)
20 mouse 108958 Miga2 mitoguardin 2 XR_001780361.1 16% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
708
ORF length:
642
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggt gacaggcctg atgaccaagg ctgagaagag ccccaaaggc ttcctggaga
121gctacgagga gatgctgagc tatgccctgc ggcccgagac ctgggccaca acacggctgg
181agctggaggg ccgaggggtg gtatgcatga gcttcttcga catcgtgctg gacttcatcc
241tcatggacgc cttcgaggac ctggagaacc ctccggcctc ggtgctcgcc gtcctgcgga
301accgctggct gtcagacagc ttcaaggaga cggccttggc cactgcttgc tggtcggtcc
361tgaaagccaa gaggaggctg ctgatggtgc ctgatggctt catctcccat ttctactccg
421tatcggagca tgtgagccct gtcctagcct tcggcttcct tggacccaag cctcagcttg
481ctgaagtctg tgctttcttc aagcaccaga ttgtgcagta cctgagggac atgttcgacc
541tggacaatgt gcgctacacg tcactgcccg cgctggcaga cgacatcctg cagctgtccc
601ggcgccgcag cgagatattg ctggggtacc tgggggtgcc cgcggccagc agcgcaggcg
661tgaatggggc gctgccccga gagaatgggc ccctggggga gctgcagtac ccaactttct
721tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
781aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

Download FASTA (ORF) (Full)