Construct: ORF ccsbBroad304_12873
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002539.1_s304c1, BRDN0000402140
- Derived from:
- ccsbBroadEn_12873
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MIGA2 (84895)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12873
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84895 | MIGA2 | mitoguardin 2 | XM_011519118.1 | 47.2% | 47.2% | 1_717del |
| 2 | human | 84895 | MIGA2 | mitoguardin 2 | NM_001329990.2 | 36% | 36% | 1_1137del |
| 3 | human | 84895 | MIGA2 | mitoguardin 2 | NM_032809.3 | 32.5% | 32.5% | 1_1329del |
| 4 | human | 84895 | MIGA2 | mitoguardin 2 | XR_929849.2 | 28.1% | 1_1363del;1630_1691del;2068_2277del | |
| 5 | human | 84895 | MIGA2 | mitoguardin 2 | NR_138422.2 | 17.4% | (many diffs) | |
| 6 | human | 84895 | MIGA2 | mitoguardin 2 | XR_929851.2 | 17.3% | 1_1434del;2077_3708del | |
| 7 | human | 84895 | MIGA2 | mitoguardin 2 | XR_001746398.1 | 17% | 1_1481del;2124_3755del | |
| 8 | human | 84895 | MIGA2 | mitoguardin 2 | XR_929850.2 | 17% | 1_1363del;1630_1767del;2144_3775del | |
| 9 | human | 84895 | MIGA2 | mitoguardin 2 | NR_138425.2 | 15.1% | 1_1407del;1674_2240del;2617_4248del | |
| 10 | human | 84895 | MIGA2 | mitoguardin 2 | NR_138424.2 | 15% | 1_1336del;1603_2245del;2622_4253del | |
| 11 | human | 84895 | MIGA2 | mitoguardin 2 | XR_001746392.1 | 15% | 1_1363del;1630_2272del;2649_4280del | |
| 12 | human | 84895 | MIGA2 | mitoguardin 2 | XR_001746395.1 | 14.7% | 1_1434del;1701_2343del;2720_4351del | |
| 13 | human | 84895 | MIGA2 | mitoguardin 2 | NR_138421.2 | 14.6% | 1_1457del;1724_2366del;2743_4374del | |
| 14 | human | 84895 | MIGA2 | mitoguardin 2 | NR_138423.2 | 14.4% | (many diffs) | |
| 15 | mouse | 108958 | Miga2 | mitoguardin 2 | XM_006497595.2 | 47.3% | 50.4% | (many diffs) |
| 16 | mouse | 108958 | Miga2 | mitoguardin 2 | NM_001242407.1 | 32% | 34.2% | (many diffs) |
| 17 | mouse | 108958 | Miga2 | mitoguardin 2 | NM_175392.3 | 32% | 34.2% | (many diffs) |
| 18 | mouse | 108958 | Miga2 | mitoguardin 2 | XM_006497593.2 | 28% | 29.8% | (many diffs) |
| 19 | mouse | 108958 | Miga2 | mitoguardin 2 | XM_006497594.2 | 22.7% | 24.3% | (many diffs) |
| 20 | mouse | 108958 | Miga2 | mitoguardin 2 | XR_001780361.1 | 16% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 708
- ORF length:
- 642
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt gacaggcctg atgaccaagg ctgagaagag ccccaaaggc ttcctggaga 121 gctacgagga gatgctgagc tatgccctgc ggcccgagac ctgggccaca acacggctgg 181 agctggaggg ccgaggggtg gtatgcatga gcttcttcga catcgtgctg gacttcatcc 241 tcatggacgc cttcgaggac ctggagaacc ctccggcctc ggtgctcgcc gtcctgcgga 301 accgctggct gtcagacagc ttcaaggaga cggccttggc cactgcttgc tggtcggtcc 361 tgaaagccaa gaggaggctg ctgatggtgc ctgatggctt catctcccat ttctactccg 421 tatcggagca tgtgagccct gtcctagcct tcggcttcct tggacccaag cctcagcttg 481 ctgaagtctg tgctttcttc aagcaccaga ttgtgcagta cctgagggac atgttcgacc 541 tggacaatgt gcgctacacg tcactgcccg cgctggcaga cgacatcctg cagctgtccc 601 ggcgccgcag cgagatattg ctggggtacc tgggggtgcc cgcggccagc agcgcaggcg 661 tgaatggggc gctgccccga gagaatgggc ccctggggga gctgcagtac ccaactttct 721 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 781 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta