Transcript: Human XR_001746395.1

PREDICTED: Homo sapiens mitoguardin 2 (MIGA2), transcript variant X5, misc_RNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
MIGA2 (84895)
Length:
4351
CDS:
(non-coding)

Additional Resources:

NCBI RefSeq record:
XR_001746395.1
NBCI Gene record:
MIGA2 (84895)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of this non-coding transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XR_001746395.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000425571 TGTTCCTGTACACGACGTTTG pLKO_005 297 3UTR 100% 6.000 8.400 N MIGA2 n/a
2 TRCN0000172533 GCCTTCAGTTCAACGCACAAA pLKO.1 3611 3UTR 100% 4.950 6.930 N MIGA2 n/a
3 TRCN0000437856 CAATGTGCGCTACACGTCACT pLKO_005 2557 3UTR 100% 2.640 3.696 N MIGA2 n/a
4 TRCN0000437106 AGATGGTGACAGGCCTGATGA pLKO_005 1433 3UTR 100% 4.950 3.465 N MIGA2 n/a
5 TRCN0000429724 CAATGCAGAGAGCCTGTACAT pLKO_005 739 3UTR 100% 4.950 3.465 N MIGA2 n/a
6 TRCN0000438002 CAGTACCTGAGGGACATGTTC pLKO_005 2528 3UTR 100% 4.950 3.465 N MIGA2 n/a
7 TRCN0000168151 CCCTTCAGTGAAGAAAGGATA pLKO.1 517 3UTR 100% 4.950 3.465 N MIGA2 n/a
8 TRCN0000179638 GCTTTCTTCAAGCACCAGATT pLKO.1 2504 3UTR 100% 4.950 3.465 N Miga2 n/a
9 TRCN0000439781 CATTCTCCCAGCTACGGTTGA pLKO_005 327 3UTR 100% 4.050 2.835 N MIGA2 n/a
10 TRCN0000432115 CCTTCTCAGAGAGTTTGCAAC pLKO_005 3180 3UTR 100% 4.050 2.835 N MIGA2 n/a
11 TRCN0000423139 CTGACTTCAGAGGATTCCTTC pLKO_005 1085 3UTR 100% 4.050 2.835 N MIGA2 n/a
12 TRCN0000172389 CTGATGGCTTCATCTCCCATT pLKO.1 2403 3UTR 100% 4.050 2.835 N MIGA2 n/a
13 TRCN0000167974 CAGTGAAGAAAGGATACTCCA pLKO.1 522 3UTR 100% 2.640 1.848 N MIGA2 n/a
14 TRCN0000168574 GAAGACAAGAGTAACCAGCTT pLKO.1 1390 3UTR 100% 2.640 1.848 N MIGA2 n/a
15 TRCN0000434518 TGCTGAAGTCTGTGCTTTCTT pLKO_005 2491 3UTR 100% 5.625 3.375 N MIGA2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XR_001746395.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12873 pDONR223 100% 14.7% None 1_1434del;1701_2343del;2720_4351del n/a
2 ccsbBroad304_12873 pLX_304 0% 14.7% V5 1_1434del;1701_2343del;2720_4351del n/a
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