Construct: ORF ccsbBroad304_12875
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017360.1_s304c1, BRDN0000401731
- Derived from:
- ccsbBroadEn_12875
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TBRG1 (84897)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12875
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84897 | TBRG1 | transforming growth factor ... | XM_017018442.2 | 71.9% | 71.9% | 1_219del |
| 2 | human | 84897 | TBRG1 | transforming growth factor ... | XM_005271709.4 | 70.8% | 70.8% | 1_231del |
| 3 | human | 84897 | TBRG1 | transforming growth factor ... | NM_032811.3 | 45.4% | 45.4% | 1_672del |
| 4 | human | 84897 | TBRG1 | transforming growth factor ... | NR_016021.2 | 11.2% | 1_755del;1317_5007del | |
| 5 | human | 84897 | TBRG1 | transforming growth factor ... | XR_428983.4 | 10.7% | 1_955del;1517_5205del | |
| 6 | human | 84897 | TBRG1 | transforming growth factor ... | XR_001748012.2 | 10.7% | 1_987del;1549_5237del | |
| 7 | human | 84897 | TBRG1 | transforming growth factor ... | XR_001748011.2 | 10.4% | 1_1107del;1669_5357del | |
| 8 | mouse | 21376 | Tbrg1 | transforming growth factor ... | NM_025289.3 | 39.5% | 37.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 630
- ORF length:
- 561
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaagtgccca gaccagaagt gtctatatac ctgtcagatc aaggatggtg 121 gtgtgcagcc tcagtttgaa attgttcctg aagatgaccc ccagaatgcc attgtcagct 181 cttctgcaga tgcttgtcat gcagaactgc tcaggactat aagcactact atggggaaac 241 taatgcctaa cctgcttcca gctggagctg acttttttgg attttctcat ccagccatcc 301 acaacctgat ccagagctgt ccaggagctc gaaaatgcat caattaccag tgggtgaaat 361 ttgatgtgtg caaacctgga gatgggcagc tacctgaggg gctgccggag aatgatgcag 421 ctatgagctt tgaagccttt cagagacaga tctttgatga agatcagaat gatccccttc 481 tgccaggatc cttggacctc ccagagcttc agcctgcagc ctttgtgtct tcttaccagc 541 ccatgtacct gacacatgaa cccttggtag atactcacct gcagcacttg aagtctccat 601 cacagggtag cccaattcag tcttcagatt tgccaacttt cttgtacaaa gtggttggta 661 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 721 ggtggcgcgt taagtcgaca atcaacctct ggatta