Construct: ORF ccsbBroad304_12904

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003245.1_s304c1, BRDN0000406875
Derived from:
ccsbBroadEn_12904
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
REPS1 (85021)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12904
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 85021 REPS1 RALBP1 associated Eps domai... NM_001286612.1 92.6% 92.6% 1_156del
2 human 85021 REPS1 RALBP1 associated Eps domai... XM_017011389.1 90.1% 90.1% 1_21del;1311_1502del
3 human 85021 REPS1 RALBP1 associated Eps domai... XM_011536202.2 86.9% 86.9% 1_21del;1123_1203del;1392_1583del
4 human 85021 REPS1 RALBP1 associated Eps domai... XM_005267179.4 85% 85% 1_156del;1446_1634del
5 human 85021 REPS1 RALBP1 associated Eps domai... NM_001128617.2 84.9% 84.9% 1_156del;1446_1637del
6 human 85021 REPS1 RALBP1 associated Eps domai... XM_017011387.2 82.2% 82.2% 1_156del;1258_1335del;1524_1712del
7 human 85021 REPS1 RALBP1 associated Eps domai... NM_031922.4 82.1% 82.1% 1_156del;1258_1338del;1527_1715del
8 human 85021 REPS1 RALBP1 associated Eps domai... XM_005267177.4 82.1% 82.1% 1_156del;1258_1335del;1524_1715del
9 human 85021 REPS1 RALBP1 associated Eps domai... NM_001286611.1 82% 82% 1_156del;1258_1338del;1527_1718del
10 human 85021 REPS1 RALBP1 associated Eps domai... XM_017011388.2 80.9% 78.6% (many diffs)
11 human 85021 REPS1 RALBP1 associated Eps domai... XM_005267178.5 76.4% 74.2% (many diffs)
12 mouse 19707 Reps1 RalBP1 associated Eps domai... NM_001111065.1 76.1% 80.4% (many diffs)
13 mouse 19707 Reps1 RalBP1 associated Eps domai... XM_017313852.1 73.6% 77.8% (many diffs)
14 mouse 19707 Reps1 RalBP1 associated Eps domai... NM_009048.2 73.5% 77.7% (many diffs)
15 mouse 19707 Reps1 RalBP1 associated Eps domai... XM_017313853.1 73.4% 77.8% (many diffs)
16 mouse 19707 Reps1 RalBP1 associated Eps domai... XM_006512621.2 70.9% 75.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2025
ORF length:
1959
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga gctttgtggt gcaacaagac ttggttattt tggaagaagt cagttctaca
121ttgctttgaa acttgtagct gttgcccagt ctggtttccc tttaagagtg gaaagtataa
181atacagtaaa ggaccttcct ctgccaagat ttgttgcttc aaagaatgaa caggaatctc
241gccatgcagc ctcatattct tcagattctg aaaatcaagg gtcgtattct ggtgtaattc
301ccccaccacc tggcaggggg caagtgaaaa agggatccgt aagccatgat acggttcagc
361ctcgtacatc tgcagatgca caggaacctg catccccagt agtttcacca cagcaatccc
421caccaacttc tccacacaca tggaggaagc acagccgtca tcccagcggt gggaatagtg
481agaggcctct cgcgggacct gggccatttt ggtctccctt tggtgaagca caatcaggtt
541cttctgctgg tgatgcagtg tggtcagggc attccccacc tccacctcaa gaaaactggg
601tcagttttgc agatactcca ccaaccagta ctcttttaac catgcatcct gcttctgtcc
661aggaccagac aacagtacga actgtagcat cagctacaac tgccattgaa attcgtaggc
721aatccagtag ttatgatgat ccctggaaaa taacagatga acaaagacag tattatgtaa
781atcagtttaa aaccattcag cctgatctaa acggatttat tccaggatct gcagctaaag
841agttttttac aaaatcaaaa cttcctattc ttgaactttc tcatatttgg gaactctcag
901actttgataa agatggtgca ttgacactgg atgagttttg tgctgctttt catctggtgg
961ttgctaggaa gaatggctat gatttaccag aaaaacttcc tgaaagctta atgcccaaac
1021tgattgattt ggaagattca gcagatgttg gggatcagcc aggtgaggta ggttattcag
1081gctctcctgc tgaagctcct ccaagcaagt caccatcgat gccatcacta aaccagacat
1141ggcctgagct gaatcagagc agtgaggata ctgctattgt tcatccagtt cccattcgta
1201tgactccaag caaaatccac atgcaggaaa tggaacttaa aagaactggc agcgatcata
1261caaatcccac tagcccatta cttgtgaaac catctgacct tttagaagaa aataagataa
1321attcatcggt gaaattcgct tctggtaata ctgtaggaca acaacaggct ggagttgttg
1381cccatcctcc tgcagtgcct ccaagaccac agccctcaca ggctcctggt cctgctgtgc
1441atcgcccagt ggatgccgat ggcctcataa ctcacactag tacctcacct cagcagatac
1501cagagcaacc aaattttgca gatttcagtc agtttgaagt atttgctgca tcaaatgtaa
1561acgacgaaca agatgatgaa gccgagaaac atccagaagt cctgccggct gaaaaagctt
1621ctgatcctgc aagttctctt cgagttgcca aaacagatag taaaactgaa gaaaagacag
1681ctgctagtgc tcctgccaat gtgagcaaag gcacaacacc acttgctcca ccacctaaac
1741ctgttcgaag aagattaaaa tcagaagatg aattaaggcc agaagttgat gaacatacac
1801aaaagacggg tgtcttagct gctgttcttg catcacaacc ttctattccc agatctgttg
1861ggaaagataa gaaagctatt caggcatcaa ttagacgtaa taaggaaacc aacaccgttt
1921tggccagatt gaatagcgaa ttgcagcaac aattaaagga tgttcttgag gagagaattt
1981ccctggaagt tcaactggaa caacttcgac cattctctca cctataccca actttcttgt
2041acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat
2101gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a

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