Transcript: Human XM_011536202.2

PREDICTED: Homo sapiens RALBP1 associated Eps domain containing 1 (REPS1), transcript variant X5, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
REPS1 (85021)
Length:
2560
CDS:
102..2357

Additional Resources:

NCBI RefSeq record:
XM_011536202.2
NBCI Gene record:
REPS1 (85021)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_011536202.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000249103 CGTAGGCAATCCAGTAGTTAT pLKO_005 771 CDS 100% 13.200 18.480 N Reps1 n/a
2 TRCN0000053364 GCGATCATACAAATCCCACTA pLKO.1 1390 CDS 100% 4.050 5.670 N REPS1 n/a
3 TRCN0000053366 GCATCAAATGTAAACGACGAA pLKO.1 1878 CDS 100% 2.640 3.696 N REPS1 n/a
4 TRCN0000053363 GCCTGATCTAAACGGATTTAT pLKO.1 857 CDS 100% 15.000 12.000 N REPS1 n/a
5 TRCN0000423162 GGCATCAATTAGACGTAATAA pLKO_005 2213 CDS 100% 15.000 10.500 N REPS1 n/a
6 TRCN0000053365 CCATTGAAATTCGTAGGCAAT pLKO.1 760 CDS 100% 4.050 2.835 N REPS1 n/a
7 TRCN0000053367 GCCCATTACTTGTGAAACCAT pLKO.1 1411 CDS 100% 3.000 2.100 N REPS1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_011536202.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_12905 pDONR223 100% 98.8% 98.6% None (many diffs) n/a
2 ccsbBroad304_12905 pLX_304 0% 98.8% 98.6% V5 (many diffs) n/a
3 TRCN0000474307 ATTCAAACGGAAAATAATATACGT pLX_317 18.1% 98.8% 98.6% V5 (many diffs) n/a
4 ccsbBroadEn_12904 pDONR223 100% 86.9% 86.9% None 1_21del;1123_1203del;1392_1583del n/a
5 ccsbBroad304_12904 pLX_304 0% 86.9% 86.9% V5 1_21del;1123_1203del;1392_1583del n/a
6 TRCN0000476941 CGAATCGGTGTTAGCTGGCTTATG pLX_317 19.3% 86.9% 86.9% V5 1_21del;1123_1203del;1392_1583del n/a
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