Construct: ORF ccsbBroad304_12905

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006360.1_s304c1, BRDN0000410431
Derived from:
ccsbBroadEn_12905
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
REPS1 (85021)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12905
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 85021 REPS1 RALBP1 associated Eps domai... XM_011536202.2 98.8% 98.6% (many diffs)
2 human 85021 REPS1 RALBP1 associated Eps domai... XM_017011389.1 95.2% 95% (many diffs)
3 human 85021 REPS1 RALBP1 associated Eps domai... NM_031922.4 93.3% 93.2% (many diffs)
4 human 85021 REPS1 RALBP1 associated Eps domai... NM_001286611.1 93.2% 93% (many diffs)
5 human 85021 REPS1 RALBP1 associated Eps domai... XM_017011387.2 93.2% 93% (many diffs)
6 human 85021 REPS1 RALBP1 associated Eps domai... XM_005267177.4 93% 92.9% (many diffs)
7 human 85021 REPS1 RALBP1 associated Eps domai... XM_017011388.2 92.5% 90.4% (many diffs)
8 human 85021 REPS1 RALBP1 associated Eps domai... XM_005267179.4 89.9% 89.8% (many diffs)
9 human 85021 REPS1 RALBP1 associated Eps domai... NM_001128617.2 89.8% 89.6% (many diffs)
10 human 85021 REPS1 RALBP1 associated Eps domai... XM_005267178.5 87.4% 85.3% (many diffs)
11 human 85021 REPS1 RALBP1 associated Eps domai... NM_001286612.1 82% 81.8% (many diffs)
12 mouse 19707 Reps1 RalBP1 associated Eps domai... NM_009048.2 84.2% 88.4% (many diffs)
13 mouse 19707 Reps1 RalBP1 associated Eps domai... XM_017313852.1 84.1% 88.3% (many diffs)
14 mouse 19707 Reps1 RalBP1 associated Eps domai... XM_006512621.2 81.6% 85.9% (many diffs)
15 mouse 19707 Reps1 RalBP1 associated Eps domai... NM_001111065.1 80.8% 85% (many diffs)
16 mouse 19707 Reps1 RalBP1 associated Eps domai... XM_017313853.1 78.2% 82.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2295
ORF length:
2229
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga gctttgtggt gcaacaagac ttggttattt tggaagaagt cagttctaca
121ttgctttgaa acttgtagct gttgcccagt ctggtttccc tttaagagtg gaaagtataa
181atacagtaaa ggaccttcct ctgccaagat ttgttgcttc aaagaatgaa caggaatctc
241gccatgcagc ctcatattct tcagattctg aaaatcaagg gtcgtattct ggtgtaattc
301ccccaccacc tggcaggggg caagtgaaaa agggatccgt aagccatgat acggttcagc
361ctcgtacatc tgcagatgca caggaacctg catccccagt agtttcacca cagcaatccc
421caccaacttc tccacacaca tggaggaagc acagccgtca tcccagcggt gggaatagtg
481agaggcctct cgcgggacct gggccatttt ggtctccctt tggtgaagca caatcaggtt
541cttctgctgg tgatgcagtg tggtcagggc attccccacc tccacctcaa gaaaactggg
601tcagttttgc agatactcca ccaaccagta ctcttttaac catgcatcct gcttctgtcc
661aggaccagac aacagtacga actgtagcat cagctacaac tgccattgaa attcgtaggc
721aatccagtag ttatgatgat ccctggaaaa taacagatga acaaagacag tattatgtaa
781atcagtttaa aaccattcag cctgatctaa acggatttat tccaggatct gcagctaaag
841agttttttac aaaatcaaaa cttcctattc ttgaactttc tcatatttgg gaactctcag
901actttgataa agatggtgca ttgacactgg atgagttttg tgctgctttt catctggtgg
961ttgctaggaa gaatggctat gatttaccag aaaaacttcc tgaaagctta atgcccaaac
1021tgattgattt ggaagattca gcagatgttg gggatcagcc aggtgaggta ggttattcag
1081gctctcctgc tgaagctcct ccaagcaagt caccatcgat gccatcacta aaccagacat
1141ggcctgagct gaatcagagc agtgagcagt gggagacatt tagtgaacgc tcttcaagct
1201cacaaactct gacccaattt gattctaaca ttgcaccagc tgatcctgat actgctattg
1261ttcatccagt tcccattcgt atgactccaa gcaaaatcca catgcaggaa atggaactta
1321aaagaactgg cagcgatcat acaaatccca ctagcccatt acttgtgaaa ccatctgacc
1381ttttagaaga aaataagata aattcatcgg tgaaattcgc ttctggtaat actgtagatg
1441gttacagtag ttcagactct tttacttctg acccagaaca gatcgggagc aatgtaactc
1501gtcaaaggtc tcattcagga acgtcgcctg ataacactgc accaccacct ccccctccaa
1561ggccacagcc ctctcattct agatcatcat ctttagatat gaatcggacc tttacagtca
1621ccacaggaca acaacaggct ggagttgttg cccatcctcc tgcagtgcct ccaagaccac
1681agccctcaca ggctcctggt cctgctgtgc atcgcccagt ggatgccgat ggcctcataa
1741ctcacactag tacctcacct cagcagatac cagagcaacc aaattttgca gatttcagtc
1801agtttgaagt atttgctgca tcaaatgtaa acgacgaaca agatgatgaa gccgagaaac
1861atccagaagt cctgccggct gaaaaagctt ctgatcctgc aagttctctt cgagttgcca
1921aaacagatag taaaactgaa gaaaagacag ctgctagtgc tcctgccaat gtgagcaaag
1981gcacaacacc acttgctcca ccacctaaac ctgttcgaag aagattaaaa tcagaagatg
2041aattaaggcc agaagttgat gaacatacac aaaagacggg tgtcttagct gctgttcttg
2101catcacaacc ttctattccc agatctgttg ggaaagataa gaaagctatt caggcatcaa
2161ttagacgtaa taaggaaacc aacaccgttt tggccagatt gaatagcgaa ttgcagcaac
2221aattaaagga tgttcttgag gagagaattt ccctggaagt tcaactggaa caacttcgac
2281cattttttcc cctataccca actttcttgt acaaagtggt tggtaagcct atccctaacc
2341ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
2401cgacaatcaa cctctggatt a

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