Construct: ORF ccsbBroad304_12931
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005943.1_s304c1, BRDN0000408435
- Derived from:
- ccsbBroadEn_12931
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- KRT8P12 (90133)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12931
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 3856 | KRT8 | keratin 8 | NM_001256293.2 | 22.2% | 17.5% | (many diffs) |
| 2 | human | 3856 | KRT8 | keratin 8 | NM_002273.4 | 22.2% | 17.5% | (many diffs) |
| 3 | human | 3856 | KRT8 | keratin 8 | NM_001256282.2 | 21% | 16.5% | (many diffs) |
| 4 | human | 283102 | KRT8P41 | keratin 8 pseudogene 41 | NR_027713.1 | 16% | (many diffs) | |
| 5 | human | 3856 | KRT8 | keratin 8 | NR_045962.2 | 15% | (many diffs) | |
| 6 | mouse | 16691 | Krt8 | keratin 8 | NM_031170.2 | 20.4% | 16.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 420
- ORF length:
- 351
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gcgaggcatc accaccatca cggtaaacca gagcctgctg agccccctta 121 acctggaggt ggactccaac attcaggctg tatacaccca ggagaaggag cagatcaaga 181 ccctcaacaa caagtttgcc tccttcacag accaggtatg gttcctggag cagcagaaca 241 aggtgctgga gaccaagtgg agccttctgc agcagcacaa gatggctggg agcaacatgg 301 acaacatgtt cgggagctat atcaacaacc ttaggcggct gctggagcca ggagaagctg 361 aggctggaag cggagcttgg caacatgcag gggctggtgg aggacttcag gaacaagtat 421 tgccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 481 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 541 ggatta