Construct: ORF ccsbBroad304_13112
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015650.1_s304c1, BRDN0000400695
- Derived from:
- ccsbBroadEn_13112
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SLC5A10 (125206)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13112
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 125206 | SLC5A10 | solute carrier family 5 mem... | NM_001282417.1 | 100% | 100% | |
| 2 | human | 125206 | SLC5A10 | solute carrier family 5 mem... | XM_011523653.3 | 90.9% | 90.9% | 1_168del |
| 3 | human | 125206 | SLC5A10 | solute carrier family 5 mem... | XM_017024191.2 | 87.2% | 87.2% | 1_168del;558_638del |
| 4 | human | 125206 | SLC5A10 | solute carrier family 5 mem... | XM_011523652.2 | 84.7% | 84.7% | 1_168del;558_638del;979_980ins48 |
| 5 | human | 125206 | SLC5A10 | solute carrier family 5 mem... | NM_001270648.2 | 82.4% | 82.4% | 1_168del;898_899ins48;1159_1160ins111 |
| 6 | human | 125206 | SLC5A10 | solute carrier family 5 mem... | NM_152351.5 | 81.5% | 81.5% | 1_168del;558_638del;1288_1289ins111 |
| 7 | human | 125206 | SLC5A10 | solute carrier family 5 mem... | NM_001042450.3 | 79% | 79% | (many diffs) |
| 8 | human | 125206 | SLC5A10 | solute carrier family 5 mem... | NM_001270649.1 | 73.4% | 73.4% | (many diffs) |
| 9 | mouse | 109342 | Slc5a10 | solute carrier family 5 (so... | NM_001033227.2 | 66.9% | 70.8% | (many diffs) |
| 10 | mouse | 109342 | Slc5a10 | solute carrier family 5 (so... | XM_006531980.3 | 66.9% | 70.8% | (many diffs) |
| 11 | mouse | 109342 | Slc5a10 | solute carrier family 5 (so... | XM_006531979.3 | 66.1% | 70% | (many diffs) |
| 12 | mouse | 109342 | Slc5a10 | solute carrier family 5 (so... | XM_017314221.1 | 63.5% | 67.2% | (many diffs) |
| 13 | mouse | 109342 | Slc5a10 | solute carrier family 5 (so... | XM_006531978.3 | 61.1% | 64.3% | (many diffs) |
| 14 | mouse | 109342 | Slc5a10 | solute carrier family 5 (so... | XM_006531981.3 | 32.7% | 33.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1764
- ORF length:
- 1698
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac gtggtggccg attggagcct ccctcttcgc cagcagcgag ggctctggcc 121 tcttcattgg actggcgggc tcaggcgcgg caggaggtct ggccgtggca ggcttcgagt 181 ggaatgccac gtacgtgctg ctggcactgg catgggtgtt cgtgcccatc tacatctcct 241 cagagatcgt caccttacct gagtacattc agaagcgcta cgggggccag cggatccgca 301 tgtacctgtc tgtcctgtcc ctgctactgt ctgtcttcac caagatatcg ctggacctgt 361 acgcgggggc tctgtttgtg cacatctgcc tgggctggaa cttctacctc tccaccatcc 421 tcacgctcgg catcacagcc ctgtacacca tcgcagcttt tgaccagatc ggtggttacg 481 ggcagctgga ggcagcctac gcccaggcca ttccctccag gaccattgcc aacaccacct 541 gccacctgcc acgtacagac gccatgcaca tgtttcgaga cccccacaca ggggacctgc 601 cgtggaccgg gatgaccttt ggcctgacca tcatggccac ctggtactgg tgcaccgacc 661 aggtcatcgt gcagcgatca ctgtcagccc gggacctgaa ccatgccaag gcgggctcca 721 tcctggccag ctacctcaag atgctcccca tgggcctgat catcatgccg ggcatgatca 781 gccgcgcatt gttcccaggt gctcatgtct atgaggagag acaccaagtg tccgtctctc 841 gaacagatga tgtgggctgc gtggtgccgt ccgagtgcct gcgggcctgc ggggccgagg 901 tcggctgctc caacatcgcc taccccaagc tggtcatgga actgatgccc atcggtctgc 961 gggggctgat gatcgcagtg atgctggcgg cgctcatgtc gtcgctgacc tccatcttca 1021 acagcagcag caccctcttc actatggaca tctggaggcg gctgcgtccc cgctccggcg 1081 agcgggagct cctgctggtg ggacgcactg ggataccctc aacaccccca gcccctcaaa 1141 gccgtctcag cttcctcctg cccgaaacac caccattgga gcggtatctc ctaggcctcg 1201 tggtcatgga tctctggctg gtcatagtgg cactcatcgg cgtgagtgtg gcctggatcc 1261 ccgtcctgca ggactccaac agcgggcaac tcttcatcta catgcagtca gtgaccagct 1321 ccctggcccc accagtgact gcagtctttg tcctgggcgt cttctggcga cgtgccaacg 1381 agcagggggc cttctggggc ctgatagcag ggctggtggt gggggccacg aggctggtcc 1441 tggaattcct gaacccagcc ccaccgtgcg gagagccaga cacgcggcca gccgtcctgg 1501 ggagcatcca ctacctgcac ttcgctgtcg ccctctttgc actcagtggt gctgttgtgg 1561 tggctggaag cctgctgacc ccacccccac agagtgtcca gattgagaac cttacctggt 1621 ggaccctggc tcaggatgtg cccttgggaa ctaaagcagg tgatggccaa acaccccaga 1681 aacacgcctt ctgggcccgt gtctgtggct tcaatgccat cctcctcatg tgtgtcaaca 1741 tattctttta tgcctacttc gcctgcccaa ctttcttgta caaagtggtt ggtaagccta 1801 tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc 1861 gcgttaagtc gacaatcaac ctctggatta