Construct: ORF ccsbBroad304_13146
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003142.1_s304c1, BRDN0000399645
- Derived from:
- ccsbBroadEn_13146
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TAF8 (129685)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13146
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 129685 | TAF8 | TATA-box binding protein as... | XM_005248849.2 | 93.6% | 93.6% | 489_490ins33 |
| 2 | human | 129685 | TAF8 | TATA-box binding protein as... | XM_017010245.1 | 80.6% | 77.4% | (many diffs) |
| 3 | human | 129685 | TAF8 | TATA-box binding protein as... | XM_017010244.2 | 77.3% | 74.3% | (many diffs) |
| 4 | human | 129685 | TAF8 | TATA-box binding protein as... | NM_138572.3 | 55.5% | 53.2% | (many diffs) |
| 5 | human | 129685 | TAF8 | TATA-box binding protein as... | XM_017010241.2 | 53.9% | 51.7% | (many diffs) |
| 6 | human | 129685 | TAF8 | TATA-box binding protein as... | XM_011514296.2 | 50.9% | 48.8% | (many diffs) |
| 7 | human | 129685 | TAF8 | TATA-box binding protein as... | XM_017010243.2 | 35.2% | 32.9% | (many diffs) |
| 8 | human | 129685 | TAF8 | TATA-box binding protein as... | XM_017010242.2 | 34.2% | 31.9% | (many diffs) |
| 9 | mouse | 63856 | Taf8 | TATA-box binding protein as... | XM_006524767.3 | 59% | 59.5% | (many diffs) |
| 10 | mouse | 63856 | Taf8 | TATA-box binding protein as... | XM_006524766.3 | 56% | 56.5% | (many diffs) |
| 11 | mouse | 63856 | Taf8 | TATA-box binding protein as... | XM_006524765.3 | 51.3% | 51.7% | (many diffs) |
| 12 | mouse | 63856 | Taf8 | TATA-box binding protein as... | NM_022015.3 | 50.8% | 51.2% | (many diffs) |
| 13 | mouse | 63856 | Taf8 | TATA-box binding protein as... | XM_006524764.2 | 48.6% | 49% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 588
- ORF length:
- 522
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc cgacgcggcg gccacagctg gggccggtgg ctccggaacg agatcgggaa 121 gtaaacagtc cactaaccct gccgataact atcatctggc ccggaggaga accctgcagg 181 tggttgtgag ctccttgctg acagaggcag ggtttgagag tgccgagaaa gcatccgtgg 241 aaacgctgac agagatgctg cagagctaca tttcagaaat tgggagaagt gccaagtctt 301 actgtgagca cacagccagg acccagccca cactgtccga tatcgtggtc acacttgttg 361 agatgggttt caatgtggac actctccctg cttatgcaaa acggtctcag aggatggtca 421 tcactgctcc tccggtgacc aatcagccag tgacccccaa ggccctcact gcagggcaga 481 accgacccca cccgccgcac atccccagcc attttcctga gttccctgat ccccacacct 541 acatcaaaac tccggtgagt gatgaagcac tgggactgcg cgtggtgtac ccaactttct 601 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 661 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta