Construct: ORF ccsbBroad304_13241

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004070.1_s304c1, BRDN0000404687
Derived from:
ccsbBroadEn_13241
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
KLC3 (147700)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13241
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 147700 KLC3 kinesin light chain 3 NM_177417.3 99.8% 99.8% 487_489delCAG
2 human 147700 KLC3 kinesin light chain 3 XM_024451371.1 90.6% 90.6% 1_153del;640_642delCAG
3 human 147700 KLC3 kinesin light chain 3 XM_024451370.1 77.1% 73% 487_489delCAG;1441_1708del;1781_1956del
4 human 147700 KLC3 kinesin light chain 3 XM_024451369.1 71.5% 67.8% (many diffs)
5 mouse 232943 Klc3 kinesin light chain 3 NM_001286038.1 85.3% 90.1% (many diffs)
6 mouse 232943 Klc3 kinesin light chain 3 NM_001286039.1 85.3% 90.1% (many diffs)
7 mouse 232943 Klc3 kinesin light chain 3 NM_146182.4 85.3% 90.1% (many diffs)
8 mouse 232943 Klc3 kinesin light chain 3 XM_017322154.1 77.6% 81% (many diffs)
9 mouse 232943 Klc3 kinesin light chain 3 XM_017322153.1 76.9% 80.3% (many diffs)
10 mouse 232943 Klc3 kinesin light chain 3 XM_006539826.3 67.6% 71.4% (many diffs)
11 mouse 232943 Klc3 kinesin light chain 3 XM_006539823.3 62.7% 65.5% (many diffs)
12 mouse 232943 Klc3 kinesin light chain 3 XM_017322152.1 60% 61% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1575
ORF length:
1509
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgtc tgtgcaggta gcggctcctg gaagtgcagg gctgggccca gagcgcctga
121gccctgagga gctggtgcgg cagacgcggc aagtggtcca ggggctggag gcgctgcggg
181cagagcacca tggcctggct gggcacctgg cggaggccct ggcgggacag ggcccggcag
241ccggcttgga gatgctggag gaaaagcagc aggtggtgag ccactcgctg gaggccatcg
301agctggggct gggcgaggcc caggtgctgc tggccctgtc ggcacatgtg ggtgcactgg
361aggcagagaa gcagcggctg cgctcgcagg cccggcggct ggcccaggag aacgtgtggc
421tgcgggagga actggaggag acgcagcggc ggcttcgggc cagcgaggag tccgtggccc
481agctggagga ggagaagcgc cacctggagt tcctggggca gctgcgacag tacgacccac
541cggcggagag ccagtctgag tccccgcctc gccgagacag cctggcctcc ctgttcccca
601gcgaggagga ggagaggaaa ggtcctgagg ccgcaggagc agcagctgct cagcagggtg
661gctatgagat ccctgcccgc cttcggaccc tgcataacct cgtgatccag tacgcggggc
721agggccgcta tgaggtggcg gtgcctctgt gccgccaggc cttggaggac ctggagcgca
781gctcgggcca ctgccaccct gacgtggcca ccatgctcaa catcctggcg ctggtgtacc
841gggaccagaa caagtacaaa gaagccacag accttctcca tgatgccctg cagatccggg
901agcagacgct gggccctgag caccccgcgg tggccgccac gctcaacaac ttggctgtcc
961tctatgggaa gcgtgggcgt taccgggagg cagagcccct gtgccagcgc gctttggaga
1021tccgagagaa ggtcctgggt gctgaccacc cagatgtggc caagcagctc aacaacctgg
1081ccctgctgtg ccagaaccag ggcaagtttg aggacgtgga gcggcactat gcccgggccc
1141tgagcatcta tgaggcactg ggcgggcccc atgaccccaa cgtggccaag accaagaaca
1201acctggcctc agcctacctg aaacagaaca agtatcaaca agcggaagag ctgtacaaag
1261aaatcctcca caaggaggac ctacccgccc ctctcggtgc ccccaacaca ggcacagctg
1321gtgacgcaga acaggccctt cgccgcagca gctcactctc caagatccgt gagtctatca
1381ggcgaggaag tgagaagctg gtctcccggc tccgaggcga ggcggcggca ggagcagccg
1441gaatgaagag agccatgtca ctcaacacac tgaacgtgga tgctccaagg gctcctggga
1501ctcagtttcc cagctggcac ctggacaagg cccctcggac cctcagcgcc agcacccagg
1561acctgagccc ccactaccca actttcttgt acaaagtggt tggtaagcct atccctaacc
1621ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt
1681cgacaatcaa cctctggatt a

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