Construct: ORF ccsbBroad304_13310

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006209.1_s304c1, BRDN0000401472
Derived from:
ccsbBroadEn_13310
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
USP54 (159195)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13310
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 159195 USP54 ubiquitin specific peptidas... XM_005269582.2 45.8% 45.8% 1_2565del;4000G>C
2 human 159195 USP54 ubiquitin specific peptidas... NM_001350995.2 45.6% 45.6% 1_2445del;3880_4021delinsC
3 human 159195 USP54 ubiquitin specific peptidas... XM_024447852.1 45.6% 45.6% 1_2445del;3880_4021delinsC
4 human 159195 USP54 ubiquitin specific peptidas... XM_024447851.1 44.7% 44.7% 1_2682del;4117G>C
5 human 159195 USP54 ubiquitin specific peptidas... NM_001320437.2 44.6% 44.4% (many diffs)
6 human 159195 USP54 ubiquitin specific peptidas... XM_017015783.1 44.5% 44.4% 1_2565del;4000_4141delinsC
7 human 159195 USP54 ubiquitin specific peptidas... XM_017015784.1 44.5% 44.4% 1_2565del;4000_4141delinsC
8 human 159195 USP54 ubiquitin specific peptidas... XM_017015782.2 44% 44% 1_2616del;4051_4192delinsC
9 human 159195 USP54 ubiquitin specific peptidas... XM_011539368.2 43.9% 43.9% 1_2631del;4066_4207delinsC
10 human 159195 USP54 ubiquitin specific peptidas... XM_024447849.1 43.6% 43.6% 1_2802del;4237G>C
11 human 159195 USP54 ubiquitin specific peptidas... XM_024447848.1 43.4% 43.4% 1_2682del;4117_4258delinsC
12 human 159195 USP54 ubiquitin specific peptidas... XM_024447850.1 43.3% 43.3% 1_2802del;4222_4223insTGCGTACTTCTCAGC
13 human 159195 USP54 ubiquitin specific peptidas... NM_152586.3 43% 42.9% 1_2736del;4171_4312delinsC
14 human 159195 USP54 ubiquitin specific peptidas... XM_017015774.1 43% 42.9% 1_2736del;4171_4312delinsC
15 human 159195 USP54 ubiquitin specific peptidas... XM_017015775.1 43% 42.9% 1_2736del;4171_4312delinsC
16 human 159195 USP54 ubiquitin specific peptidas... XM_017015777.1 43% 42.9% 1_2736del;4171_4312delinsC
17 human 159195 USP54 ubiquitin specific peptidas... XM_024447847.1 43% 42.9% 1_2736del;4171_4312delinsC
18 human 159195 USP54 ubiquitin specific peptidas... XM_024447846.1 42.5% 42.3% (many diffs)
19 human 159195 USP54 ubiquitin specific peptidas... XM_024447832.1 42.4% 42.4% 1_2802del;4237_4378delinsC
20 human 159195 USP54 ubiquitin specific peptidas... XM_024447833.1 42.4% 42.4% 1_2802del;4237_4378delinsC
21 human 159195 USP54 ubiquitin specific peptidas... XM_024447834.1 42.4% 42.4% 1_2802del;4237_4378delinsC
22 human 159195 USP54 ubiquitin specific peptidas... XM_024447835.1 42.4% 42.4% 1_2802del;4237_4378delinsC
23 human 159195 USP54 ubiquitin specific peptidas... XM_024447836.1 42.4% 42.4% 1_2802del;4237_4378delinsC
24 human 159195 USP54 ubiquitin specific peptidas... XM_024447837.1 42.4% 42.4% 1_2802del;4237_4378delinsC
25 human 159195 USP54 ubiquitin specific peptidas... XM_024447838.1 42.4% 42.4% 1_2802del;4237_4378delinsC
26 human 159195 USP54 ubiquitin specific peptidas... XM_024447839.1 42.4% 42.4% 1_2802del;4237_4378delinsC
27 human 159195 USP54 ubiquitin specific peptidas... XM_024447840.1 42.4% 42.4% 1_2802del;4237_4378delinsC
28 human 159195 USP54 ubiquitin specific peptidas... XM_024447841.1 42.4% 42.4% 1_2802del;4237_4378delinsC
29 human 159195 USP54 ubiquitin specific peptidas... XM_024447842.1 42.4% 42.4% 1_2802del;4237_4378delinsC
30 human 159195 USP54 ubiquitin specific peptidas... XM_024447843.1 42.4% 42.4% 1_2802del;4237_4378delinsC
31 human 159195 USP54 ubiquitin specific peptidas... XM_024447844.1 42.4% 42.4% 1_2802del;4237_4378delinsC
32 human 159195 USP54 ubiquitin specific peptidas... XM_024447845.1 42.4% 42.4% 1_2802del;4237_4378delinsC
33 human 159195 USP54 ubiquitin specific peptidas... NR_135250.1 39% (many diffs)
34 human 159195 USP54 ubiquitin specific peptidas... XR_001747048.1 37.8% 1_2331del;3766_3907delinsC;4648_5741del
35 human 159195 USP54 ubiquitin specific peptidas... XR_001747042.1 36.7% 1_2502del;3937_4078delinsC;4819_5912del
36 human 159195 USP54 ubiquitin specific peptidas... NR_146998.2 36.2% 1_2728del;4163G>C;4904_5997del
37 human 159195 USP54 ubiquitin specific peptidas... NR_146997.2 35.4% 1_2728del;4163_4304delinsC;5045_6138del
38 human 159195 USP54 ubiquitin specific peptidas... NR_135249.2 33.9% 1_3132del;4567G>C;5308_6401del
39 human 159195 USP54 ubiquitin specific peptidas... XR_001747041.2 31.8% 1_3407del;4842_4983delinsC;5724_6817del
40 human 159195 USP54 ubiquitin specific peptidas... XR_001747037.2 28.9% 1_4093del;5528_5669delinsC;6410_7503del
41 human 159195 USP54 ubiquitin specific peptidas... XR_001747046.1 27.8% 1_4385del;5820_5961delinsC;6702_7795del
42 human 159195 USP54 ubiquitin specific peptidas... XR_001747043.1 27.7% 1_4556del;5991G>C;6732_7825del
43 human 159195 USP54 ubiquitin specific peptidas... XR_001747035.1 27% 1_4622del;6057_6198delinsC;6939_8032del
44 human 159195 USP54 ubiquitin specific peptidas... XR_001747038.1 25.6% 1_5055del;6490_6631delinsC;7372_8465del
45 human 159195 USP54 ubiquitin specific peptidas... XR_001747047.1 24.3% (many diffs)
46 human 159195 USP54 ubiquitin specific peptidas... XR_001747039.1 24.2% 1_5686del;7121G>C;7862_8955del
47 human 159195 USP54 ubiquitin specific peptidas... XR_002956962.1 24.1% 1_5581del;7016_7157delinsC;7898_8991del
48 human 159195 USP54 ubiquitin specific peptidas... XR_001747044.1 24.1% 1_5686del;7106_7107insTGCGTACTTCTCAGC;7847_8940del
49 human 159195 USP54 ubiquitin specific peptidas... XR_001747036.1 24% 1_5752del;7187G>C;7928_9021del
50 human 159195 USP54 ubiquitin specific peptidas... XR_002956959.1 23.7% 1_5752del;7187_7328delinsC;8069_9162del
51 human 159195 USP54 ubiquitin specific peptidas... XR_002956960.1 20.6% 1_5752del;7172_7173insTGCGTACTTCTCAGC;7386_7387ins526
52 human 159195 USP54 ubiquitin specific peptidas... XR_002956961.1 18.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2244
ORF length:
2175
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggatacagag tttggggcca gttctttctt ccattcacct gcttcctgcc
121atgagtcaca ctcatcacta tctccagagt catctgcccc acagcacagc tcccccagta
181gatctgcctt gaagcttctg acttcggttg aagtagacaa cattgaaccc tctgcattcc
241acaggcaagg tttacctaaa gcaccagggt ggactgagaa gaattctcat catagttggg
301agccattgga tgccccagag ggtaagctgc aaggctctag gtgtgacaac agcagttgca
361gcaagctccc tccacaagaa ggaagaggca ttgctcaaga acagctgttc caagaaaaga
421aggatcctgc taacccctcc ccggtgatgc ctggaatagc cacctctgag aggggtgatg
481aacacagcct aggctgtagt ccttcaaatt catcagctca gcccagcctt cccctgtata
541gaacctgcca ccccataatg cctgttgctt cttcatttgt gcttcactgt cctgatcctg
601tgcagaaaac taaccaatgc ctccaaggcc aaagcctcaa aacttcattg actttaaaag
661tggacagagg cagtgaggag acctataggc cagagtttcc cagcacaaag gggcttgtcc
721gttctctggc tgagcagttc cagaggatgc agggtgtctc catgagggat agtacaggtt
781tcaaggatag aagtttgtca ggtagtctaa ggaagaactc ttccccttct gattctaagc
841ctcctttctc acagggtcaa gagaaaggcc actggccatg ggcaaagcaa caatcctctc
901tggagggtgg ggatagacca ctttcctggg aagagtccac tgaacattct tctcttgcct
961taaactctgg gctgcctaat ggtgaaactt ctagcggagg acagcccagg ttggcagagc
1021cagacatata ccaagagaag ctgtcccaag tgagagatgt taggtctaag gatctgggca
1081gcagtactga cttggggact tccttgcctt tggattcctg ggtgaatatc acaaggttct
1141gtgattctca gcttaagcat ggggcaccta ggccaggaat gaagtcctcc cctcatgatt
1201cccatacgtg tgtaacctat ccagagagaa atcacatcct tttgcatcca cattggaacc
1261aagacacaga gcaggagacc tcagaattgg agtctctgta tcaggccagt cttcaggctt
1321ctcaagctgg ctgttctgga tgggggcagc aggataccgc ctggcaccca cttagccaaa
1381caggctctgc agatggcatg gggaggaggt tgcactcagc ccatgatcct ggtctctcaa
1441agacttcaac agcagaaatg gagcatggtc tccatgaagc cagaacagtg cgtacttctc
1501agcattcatc aaacgtgagg aagcctttgg aaaccgggca ccgttgttcc agctcctctt
1561ccctccctgt catccatgac ccttctgtgt ttctcctcgg tccccaactc taccttcccc
1621aaccacagtt cctgtcccca gatgtcctga tgcccaccat ggcaggggag cccaatagac
1681tcccaggaac ttcaaggagt gtccagcagt ttctggctat gtgtgacagg ggtgaaactt
1741cccaaggggc caagtacaca ggaaggactt tgaactacca gagcctcccc catcgctcca
1801gaacagacaa ctcctgggca ccctggtcag agaccaacca gcatattggg accagattcc
1861tgactactcc agggtgcaat cctcaactaa cctacactgc cacactacca gaaagaagca
1921agggccttca ggttcctcac actcagtcct ggagtgatct tttccattca ccctcccacc
1981ctcccattgt tcatcctgtg tacccaccat ctagcagtct tcatgtaccc ctgaggtcag
2041cttggaattc agatcctgtt ccagggtccc gaacccctgg tcctcgaaga gtagatatgc
2101ccccagatga tgactggagg caaagcagtt atgcctccca ctctggacac aggagaacag
2161tgggagaggg gtttctgttt gttctatcag atgctcccag aagagagcag atcagggcta
2221gagtcctgca gcacagtcaa tggttgccaa ctttcttgta caaagtggtt ggtaagccta
2281tccctaaccc tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc
2341gcgttaagtc gacaatcaac ctctggatta

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.