Construct: ORF ccsbBroad304_13386

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF009360.1_s304c1, BRDN0000401986
Derived from:
ccsbBroadEn_13386
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CEP112 (201134)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13386
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 201134 CEP112 centrosomal protein 112 NM_001302891.3 99.9% 99.8% 1287T>C;1525A>G
2 human 201134 CEP112 centrosomal protein 112 NM_001353128.2 99.9% 99.8% 1287T>C;1525A>G
3 human 201134 CEP112 centrosomal protein 112 NM_001199165.4 95.5% 95.4% 642_767del;1413T>C;1651A>G
4 human 201134 CEP112 centrosomal protein 112 NM_001353127.1 95.5% 95.4% 642_767del;1413T>C;1651A>G
5 human 201134 CEP112 centrosomal protein 112 NM_001353129.2 95.4% 95.3% 642_770del;1416T>C;1654A>G
6 human 201134 CEP112 centrosomal protein 112 XM_005257119.5 95.4% 95.3% 642_770del;1416T>C;1654A>G
7 human 201134 CEP112 centrosomal protein 112 XM_011524462.3 90.3% 89.6% (many diffs)
8 human 201134 CEP112 centrosomal protein 112 XM_011524463.2 89.7% 89.5% (many diffs)
9 human 201134 CEP112 centrosomal protein 112 XM_011524464.3 88% 87.9% (many diffs)
10 human 201134 CEP112 centrosomal protein 112 XM_011524465.2 78.1% 77.2% (many diffs)
11 human 201134 CEP112 centrosomal protein 112 XM_024450634.1 78.1% 77.2% (many diffs)
12 human 201134 CEP112 centrosomal protein 112 XR_934412.2 75.6% (many diffs)
13 human 201134 CEP112 centrosomal protein 112 XR_001752444.1 72.9% (many diffs)
14 human 201134 CEP112 centrosomal protein 112 XM_006721744.3 72.3% 70.7% (many diffs)
15 human 201134 CEP112 centrosomal protein 112 XR_934414.2 71.5% (many diffs)
16 human 201134 CEP112 centrosomal protein 112 XM_005257125.3 70.9% 69.3% (many diffs)
17 human 201134 CEP112 centrosomal protein 112 XM_011524466.2 65.9% 65.4% (many diffs)
18 human 201134 CEP112 centrosomal protein 112 XM_011524467.2 60% 60% (many diffs)
19 human 201134 CEP112 centrosomal protein 112 XM_005257126.4 59.8% 59.8% 0_1ins1098;189T>C;427A>G
20 human 201134 CEP112 centrosomal protein 112 NM_001037325.3 20.8% 18.8% (many diffs)
21 mouse 76380 Cep112 centrosomal protein 112 NM_029606.3 82.1% 82.8% (many diffs)
22 mouse 76380 Cep112 centrosomal protein 112 XM_017314819.1 78% 78.7% (many diffs)
23 mouse 76380 Cep112 centrosomal protein 112 XM_006534425.3 77.9% 78.5% (many diffs)
24 mouse 76380 Cep112 centrosomal protein 112 XM_006534424.3 77.2% 77.8% (many diffs)
25 mouse 76380 Cep112 centrosomal protein 112 XM_006534423.3 76.1% 76.7% (many diffs)
26 mouse 76380 Cep112 centrosomal protein 112 XM_011249300.2 66.3% 67.1% (many diffs)
27 mouse 76380 Cep112 centrosomal protein 112 XM_011249301.2 52.4% 53% (many diffs)
28 mouse 76380 Cep112 centrosomal protein 112 NM_145688.2 16.8% 15.8% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2808
ORF length:
2739
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggaagtggga agtgaagaag aaaaatggga gaagctggat gcagaatttg
121atcactttgt ggttgatatg aagccctttg ttctaaaatt acctcatagg acagaacggc
181agaggtgtgc tctttggatt agaaagctgt gcgaaccttc aggaacaggt gcaggaataa
241tggggaggaa gaatcggaac ctgtatgcaa aattgttatt gcatatgctt aaacgaggtg
301cgcttgaagg cccttttaca caccgacctg aacccgggac actaaaaatt ctaccttcat
361acatgtccat ctattttgat gaaccaaatc cagcacgagc aaaaggttca agcccagagg
421gattaccagc ctgggtactg ggtgagctgg agacaagtga acacaaatta aatgaatcat
481ggaaactctc ttctggagaa gataacactt tagtacagtc gccaactgat gtctacagca
541gggaacagta cactgggaag ctccgagtga gatcacactc cttgagtcca actcacagag
601aagatggaca aaatattacc ccaaagattt gtgaagttta ttcgaaaaaa tctcctgttt
661ctttggatga tagtgacatt gaagctcgcc ttaatagttg gaatcttggg ctggacatga
721aaacaaaaat gatggaagct aaatttcatg aagaaaagct taaactgcaa cagaaacatg
781atgctgatgt tcagaagatt ttagaaagaa agaataatga aatagaagaa ctgaaaactt
841tatacaggag taaacaacat gaaactgaag agactattag aaagcttgaa aagaaagttc
901agacactgat acgtgactgt caagttatca gagagactaa agaagaccag attgcagagc
961tgaaaaagat atgtgaacaa agtacggaat ctctaaataa tgactgggaa aaaaagcttc
1021acaatgctgt agcagaaatg gaacaggaaa agtttgatct tcaaaagcaa cacactgaaa
1081acattcaaga attgcttgag gatacaaatg tgcgtctgaa taaaatggag agtgaataca
1141tggcgcaaac acagtccaca aaccacatga tcaaagaact ggaggcccgt gtccagcagc
1201tgactggaga agcagagaac agtaatttac agaggcagaa attaattcaa gaaaaagcag
1261aacttgaaag atgttaccag ataacgtgta gtgaattaca agaagtaaag gcaaggcgta
1321acacactgca taaagagaag gaccatcttg taaacgatta tgagcaaaac atgaaactgt
1381tacaaaccaa atatgatgct gatataaacc ttctaaaaca agaacatgct ctttcagctt
1441ctaaggcatc tagtatgatt gaagaattag agcagaatgt ctgtcaatta aaacagcagt
1501tacaggaatc agaacttcaa agaaagcaac aactaaggga tcaagaaaat aagtttcaga
1561tggagaaaag tcatttaaaa cacatctatg aagaaaaggc tcatgacttg cagagtgaac
1621ttgataaagg aaaagaagat actcaaaaga aaattcataa atttgaggaa gctttgaagg
1681aaaaagagga gcagctaact cgtgtgactg aagttcagag gttgcaggcc cagcaggcag
1741atgccgctct ggaagagttt aagcggcagg tggaactgaa ctcagagaaa gtctatgctg
1801aaatgaaaga gcagatggaa aaagtggagg cagatctaac tagatccaaa tctcttcgtg
1861agaaacaatc aaaggagttt ttatggcaac tggaggacat cagacagcgg tatgaacaac
1921agatagtaga gctgaagctg gagcatgaac aggagaagac gcacctatta cagcagcata
1981acgcagagaa ggatagccta gtccgagacc atgaacggga aattgaaaac ctggaaaaac
2041agcttcgcgc tgccaatatg gagcacgaaa atcaaattca ggagttcaag aaacgagatg
2101cacaggttat tgccgacatg gaggcccagg ttcacaagtt gagagaagaa ttgatcaatg
2161tgaactcaca gcggaaacag cagctggtag agcttggtct tcttcgtgaa gaggaaaagc
2221aaagggctac aagggaacat gagattgtcg tcaataaact gaaggctgaa tcagaaaaga
2281tgaaaataga gctgaaaaag actcatgctg ctgagacaga gatgacactg gaaaaggcca
2341acagcaagct gaagcagatt gagaaggaat acactcagaa gcttgccaaa tcttcacaga
2401tcatagcaga acttcagaca accatttcct ctctgaaaga agagaacagc cagcagcagc
2461ttgctgcaga aaggcggctc caggatgtta gacaaaagtt tgaagatgag aagaagcagc
2521tgattagaga taatgaccaa gcaatcaagg ttttacaaga tgaattagaa aaccgttcta
2581atcaggtgcg atgtgcagag aaaaaattac aacacaaaga attggagtca caggaacaga
2641taacttacat acgacaagaa tatgaaacaa aattgaaagg attgatgcca gcatccctaa
2701gacaagaact tgaagacacc atttcctccc taaaatcaca ggttaatttt ctgcaaaaga
2761gagcttccat ccttcaggaa gaactgacta catatcaagg cagaaggttg ccaactttct
2821tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
2881aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

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