Construct: ORF ccsbBroad304_13386
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF009360.1_s304c1, BRDN0000401986
- Derived from:
- ccsbBroadEn_13386
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CEP112 (201134)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13386
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 201134 | CEP112 | centrosomal protein 112 | NM_001302891.3 | 99.9% | 99.8% | 1287T>C;1525A>G |
| 2 | human | 201134 | CEP112 | centrosomal protein 112 | NM_001353128.2 | 99.9% | 99.8% | 1287T>C;1525A>G |
| 3 | human | 201134 | CEP112 | centrosomal protein 112 | NM_001199165.4 | 95.5% | 95.4% | 642_767del;1413T>C;1651A>G |
| 4 | human | 201134 | CEP112 | centrosomal protein 112 | NM_001353127.1 | 95.5% | 95.4% | 642_767del;1413T>C;1651A>G |
| 5 | human | 201134 | CEP112 | centrosomal protein 112 | NM_001353129.2 | 95.4% | 95.3% | 642_770del;1416T>C;1654A>G |
| 6 | human | 201134 | CEP112 | centrosomal protein 112 | XM_005257119.5 | 95.4% | 95.3% | 642_770del;1416T>C;1654A>G |
| 7 | human | 201134 | CEP112 | centrosomal protein 112 | XM_011524462.3 | 90.3% | 89.6% | (many diffs) |
| 8 | human | 201134 | CEP112 | centrosomal protein 112 | XM_011524463.2 | 89.7% | 89.5% | (many diffs) |
| 9 | human | 201134 | CEP112 | centrosomal protein 112 | XM_011524464.3 | 88% | 87.9% | (many diffs) |
| 10 | human | 201134 | CEP112 | centrosomal protein 112 | XM_011524465.2 | 78.1% | 77.2% | (many diffs) |
| 11 | human | 201134 | CEP112 | centrosomal protein 112 | XM_024450634.1 | 78.1% | 77.2% | (many diffs) |
| 12 | human | 201134 | CEP112 | centrosomal protein 112 | XR_934412.2 | 75.6% | (many diffs) | |
| 13 | human | 201134 | CEP112 | centrosomal protein 112 | XR_001752444.1 | 72.9% | (many diffs) | |
| 14 | human | 201134 | CEP112 | centrosomal protein 112 | XM_006721744.3 | 72.3% | 70.7% | (many diffs) |
| 15 | human | 201134 | CEP112 | centrosomal protein 112 | XR_934414.2 | 71.5% | (many diffs) | |
| 16 | human | 201134 | CEP112 | centrosomal protein 112 | XM_005257125.3 | 70.9% | 69.3% | (many diffs) |
| 17 | human | 201134 | CEP112 | centrosomal protein 112 | XM_011524466.2 | 65.9% | 65.4% | (many diffs) |
| 18 | human | 201134 | CEP112 | centrosomal protein 112 | XM_011524467.2 | 60% | 60% | (many diffs) |
| 19 | human | 201134 | CEP112 | centrosomal protein 112 | XM_005257126.4 | 59.8% | 59.8% | 0_1ins1098;189T>C;427A>G |
| 20 | human | 201134 | CEP112 | centrosomal protein 112 | NM_001037325.3 | 20.8% | 18.8% | (many diffs) |
| 21 | mouse | 76380 | Cep112 | centrosomal protein 112 | NM_029606.3 | 82.1% | 82.8% | (many diffs) |
| 22 | mouse | 76380 | Cep112 | centrosomal protein 112 | XM_017314819.1 | 78% | 78.7% | (many diffs) |
| 23 | mouse | 76380 | Cep112 | centrosomal protein 112 | XM_006534425.3 | 77.9% | 78.5% | (many diffs) |
| 24 | mouse | 76380 | Cep112 | centrosomal protein 112 | XM_006534424.3 | 77.2% | 77.8% | (many diffs) |
| 25 | mouse | 76380 | Cep112 | centrosomal protein 112 | XM_006534423.3 | 76.1% | 76.7% | (many diffs) |
| 26 | mouse | 76380 | Cep112 | centrosomal protein 112 | XM_011249300.2 | 66.3% | 67.1% | (many diffs) |
| 27 | mouse | 76380 | Cep112 | centrosomal protein 112 | XM_011249301.2 | 52.4% | 53% | (many diffs) |
| 28 | mouse | 76380 | Cep112 | centrosomal protein 112 | NM_145688.2 | 16.8% | 15.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2808
- ORF length:
- 2739
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggaagtggga agtgaagaag aaaaatggga gaagctggat gcagaatttg 121 atcactttgt ggttgatatg aagccctttg ttctaaaatt acctcatagg acagaacggc 181 agaggtgtgc tctttggatt agaaagctgt gcgaaccttc aggaacaggt gcaggaataa 241 tggggaggaa gaatcggaac ctgtatgcaa aattgttatt gcatatgctt aaacgaggtg 301 cgcttgaagg cccttttaca caccgacctg aacccgggac actaaaaatt ctaccttcat 361 acatgtccat ctattttgat gaaccaaatc cagcacgagc aaaaggttca agcccagagg 421 gattaccagc ctgggtactg ggtgagctgg agacaagtga acacaaatta aatgaatcat 481 ggaaactctc ttctggagaa gataacactt tagtacagtc gccaactgat gtctacagca 541 gggaacagta cactgggaag ctccgagtga gatcacactc cttgagtcca actcacagag 601 aagatggaca aaatattacc ccaaagattt gtgaagttta ttcgaaaaaa tctcctgttt 661 ctttggatga tagtgacatt gaagctcgcc ttaatagttg gaatcttggg ctggacatga 721 aaacaaaaat gatggaagct aaatttcatg aagaaaagct taaactgcaa cagaaacatg 781 atgctgatgt tcagaagatt ttagaaagaa agaataatga aatagaagaa ctgaaaactt 841 tatacaggag taaacaacat gaaactgaag agactattag aaagcttgaa aagaaagttc 901 agacactgat acgtgactgt caagttatca gagagactaa agaagaccag attgcagagc 961 tgaaaaagat atgtgaacaa agtacggaat ctctaaataa tgactgggaa aaaaagcttc 1021 acaatgctgt agcagaaatg gaacaggaaa agtttgatct tcaaaagcaa cacactgaaa 1081 acattcaaga attgcttgag gatacaaatg tgcgtctgaa taaaatggag agtgaataca 1141 tggcgcaaac acagtccaca aaccacatga tcaaagaact ggaggcccgt gtccagcagc 1201 tgactggaga agcagagaac agtaatttac agaggcagaa attaattcaa gaaaaagcag 1261 aacttgaaag atgttaccag ataacgtgta gtgaattaca agaagtaaag gcaaggcgta 1321 acacactgca taaagagaag gaccatcttg taaacgatta tgagcaaaac atgaaactgt 1381 tacaaaccaa atatgatgct gatataaacc ttctaaaaca agaacatgct ctttcagctt 1441 ctaaggcatc tagtatgatt gaagaattag agcagaatgt ctgtcaatta aaacagcagt 1501 tacaggaatc agaacttcaa agaaagcaac aactaaggga tcaagaaaat aagtttcaga 1561 tggagaaaag tcatttaaaa cacatctatg aagaaaaggc tcatgacttg cagagtgaac 1621 ttgataaagg aaaagaagat actcaaaaga aaattcataa atttgaggaa gctttgaagg 1681 aaaaagagga gcagctaact cgtgtgactg aagttcagag gttgcaggcc cagcaggcag 1741 atgccgctct ggaagagttt aagcggcagg tggaactgaa ctcagagaaa gtctatgctg 1801 aaatgaaaga gcagatggaa aaagtggagg cagatctaac tagatccaaa tctcttcgtg 1861 agaaacaatc aaaggagttt ttatggcaac tggaggacat cagacagcgg tatgaacaac 1921 agatagtaga gctgaagctg gagcatgaac aggagaagac gcacctatta cagcagcata 1981 acgcagagaa ggatagccta gtccgagacc atgaacggga aattgaaaac ctggaaaaac 2041 agcttcgcgc tgccaatatg gagcacgaaa atcaaattca ggagttcaag aaacgagatg 2101 cacaggttat tgccgacatg gaggcccagg ttcacaagtt gagagaagaa ttgatcaatg 2161 tgaactcaca gcggaaacag cagctggtag agcttggtct tcttcgtgaa gaggaaaagc 2221 aaagggctac aagggaacat gagattgtcg tcaataaact gaaggctgaa tcagaaaaga 2281 tgaaaataga gctgaaaaag actcatgctg ctgagacaga gatgacactg gaaaaggcca 2341 acagcaagct gaagcagatt gagaaggaat acactcagaa gcttgccaaa tcttcacaga 2401 tcatagcaga acttcagaca accatttcct ctctgaaaga agagaacagc cagcagcagc 2461 ttgctgcaga aaggcggctc caggatgtta gacaaaagtt tgaagatgag aagaagcagc 2521 tgattagaga taatgaccaa gcaatcaagg ttttacaaga tgaattagaa aaccgttcta 2581 atcaggtgcg atgtgcagag aaaaaattac aacacaaaga attggagtca caggaacaga 2641 taacttacat acgacaagaa tatgaaacaa aattgaaagg attgatgcca gcatccctaa 2701 gacaagaact tgaagacacc atttcctccc taaaatcaca ggttaatttt ctgcaaaaga 2761 gagcttccat ccttcaggaa gaactgacta catatcaagg cagaaggttg ccaactttct 2821 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 2881 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta