Construct: ORF ccsbBroad304_13449

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF001410.1_s304c1, BRDN0000408905
Derived from:
ccsbBroadEn_13449
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
RICTOR (253260)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13449
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 253260 RICTOR RPTOR independent companion... XM_006714463.3 15.7% 2.8% (many diffs)
2 human 253260 RICTOR RPTOR independent companion... XM_011514005.2 15.4% 2.8% (many diffs)
3 human 253260 RICTOR RPTOR independent companion... NM_152756.5 15.3% 2.8% (many diffs)
4 human 253260 RICTOR RPTOR independent companion... NM_001285439.2 15.1% 2.7% (many diffs)
5 human 253260 RICTOR RPTOR independent companion... XM_017009312.1 14.4% 1.8% (many diffs)
6 human 253260 RICTOR RPTOR independent companion... XM_017009311.1 14.2% 1.8% (many diffs)
7 human 253260 RICTOR RPTOR independent companion... XM_017009313.1 12% .7% (many diffs)
8 human 253260 RICTOR RPTOR independent companion... XM_011514006.3 11.4% .7% (many diffs)
9 mouse 78757 Rictor RPTOR independent companion... XM_006520004.3 18.7% 3.6% (many diffs)
10 mouse 78757 Rictor RPTOR independent companion... NM_030168.3 14% 2.7% (many diffs)
11 mouse 78757 Rictor RPTOR independent companion... XM_006520003.1 13.8% 2.7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
249
ORF length:
183
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc ggcgatcggc cgcggccgct ctctgaagaa cctccgagta cgagggcgga
121atgacagcgg cgaggagaac gtcccgctgg atctgacccg agaaccttct gataacttaa
181gagagattcT CCAAAATGTG GCCCAGATTG CAGGGAGTAT CAAATATGAG AAAGCTAGGC
241CATCTGAATA ACTTTACTAA GCTTCTTTGT GATATTGGCC ACAGTGAAGA AAAACTGGGC
301TTTCACTATG AGGATATCAT AATTTGTTTG CGGTTAGCTT TATTAAATGA AGCAAAAGAA
361GTGCGAGCAG CAGGGCTACG AGCGCTTCGA TATCTCATCC AAGACTCCAG TATTCTCCAG
421AAGGTGCTAA AATTGAAAGT GGACTATTTA ATAGCTAGGT GCATTGACAT ACAACAGAGC
481AACGAGGTAG AGAGGACACA AGCACTTCGA TTAGTCAGAA AGATGATTAC TGTGAATGCT
541TCCTTGTTTC CTAGTTCTGT GACCAACTCA TTAATTGCAG TTGGAAATGA TGGACTTCAA
601GAAAGAGACA GAATGGTCCG AGCATGCATT GCCATTATCT GTGAACTAGC ACTTCAGAAT
661CCAGAGGTGG TGGCCCTTCG AGGTGGACTA AACACCATCT TGAAAAATGT GATCGATTGC
721CAATTAAGTC GAATAAATGA GGCCCTAATT ACTACAATTT TGCACCTTCT TAATCATCCA
781AAGACTCGAC AGTATGTGCG AGCTGATGTA GAATTAGAGG TAGGAACTTT AATATTCTTT
841TCTAGTTTTA AAATATTATT ATACCCAACT TTCTTGTACa aagtggttgg taagcctatc
901cctaaccctc tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc
961gttaagtcga caatcaacct ctggatta

Download FASTA (ORF) (Full)

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