Construct: ORF ccsbBroad304_13449
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001410.1_s304c1, BRDN0000408905
- Derived from:
- ccsbBroadEn_13449
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- RICTOR (253260)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13449
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 253260 | RICTOR | RPTOR independent companion... | XM_006714463.3 | 15.7% | 2.8% | (many diffs) |
| 2 | human | 253260 | RICTOR | RPTOR independent companion... | XM_011514005.2 | 15.4% | 2.8% | (many diffs) |
| 3 | human | 253260 | RICTOR | RPTOR independent companion... | NM_152756.5 | 15.3% | 2.8% | (many diffs) |
| 4 | human | 253260 | RICTOR | RPTOR independent companion... | NM_001285439.2 | 15.1% | 2.7% | (many diffs) |
| 5 | human | 253260 | RICTOR | RPTOR independent companion... | XM_017009312.1 | 14.4% | 1.8% | (many diffs) |
| 6 | human | 253260 | RICTOR | RPTOR independent companion... | XM_017009311.1 | 14.2% | 1.8% | (many diffs) |
| 7 | human | 253260 | RICTOR | RPTOR independent companion... | XM_017009313.1 | 12% | .7% | (many diffs) |
| 8 | human | 253260 | RICTOR | RPTOR independent companion... | XM_011514006.3 | 11.4% | .7% | (many diffs) |
| 9 | mouse | 78757 | Rictor | RPTOR independent companion... | XM_006520004.3 | 18.7% | 3.6% | (many diffs) |
| 10 | mouse | 78757 | Rictor | RPTOR independent companion... | NM_030168.3 | 14% | 2.7% | (many diffs) |
| 11 | mouse | 78757 | Rictor | RPTOR independent companion... | XM_006520003.1 | 13.8% | 2.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 249
- ORF length:
- 183
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggcgatcggc cgcggccgct ctctgaagaa cctccgagta cgagggcgga 121 atgacagcgg cgaggagaac gtcccgctgg atctgacccg agaaccttct gataacttaa 181 gagagattcT CCAAAATGTG GCCCAGATTG CAGGGAGTAT CAAATATGAG AAAGCTAGGC 241 CATCTGAATA ACTTTACTAA GCTTCTTTGT GATATTGGCC ACAGTGAAGA AAAACTGGGC 301 TTTCACTATG AGGATATCAT AATTTGTTTG CGGTTAGCTT TATTAAATGA AGCAAAAGAA 361 GTGCGAGCAG CAGGGCTACG AGCGCTTCGA TATCTCATCC AAGACTCCAG TATTCTCCAG 421 AAGGTGCTAA AATTGAAAGT GGACTATTTA ATAGCTAGGT GCATTGACAT ACAACAGAGC 481 AACGAGGTAG AGAGGACACA AGCACTTCGA TTAGTCAGAA AGATGATTAC TGTGAATGCT 541 TCCTTGTTTC CTAGTTCTGT GACCAACTCA TTAATTGCAG TTGGAAATGA TGGACTTCAA 601 GAAAGAGACA GAATGGTCCG AGCATGCATT GCCATTATCT GTGAACTAGC ACTTCAGAAT 661 CCAGAGGTGG TGGCCCTTCG AGGTGGACTA AACACCATCT TGAAAAATGT GATCGATTGC 721 CAATTAAGTC GAATAAATGA GGCCCTAATT ACTACAATTT TGCACCTTCT TAATCATCCA 781 AAGACTCGAC AGTATGTGCG AGCTGATGTA GAATTAGAGG TAGGAACTTT AATATTCTTT 841 TCTAGTTTTA AAATATTATT ATACCCAACT TTCTTGTACa aagtggttgg taagcctatc 901 cctaaccctc tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc 961 gttaagtcga caatcaacct ctggatta