Transcript: Human XM_017009312.1

PREDICTED: Homo sapiens RPTOR independent companion of MTOR complex 2 (RICTOR), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
RICTOR (253260)
Length:
9470
CDS:
13..5091

Additional Resources:

NCBI RefSeq record:
XM_017009312.1
NBCI Gene record:
RICTOR (253260)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017009312.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000074289 CGGAGGTTCATACAAGAATTA pLKO.1 4972 CDS 100% 13.200 18.480 N RICTOR n/a
2 TRCN0000289691 CGGAGGTTCATACAAGAATTA pLKO_005 4972 CDS 100% 13.200 18.480 N RICTOR n/a
3 TRCN0000074291 CGTCGGAGTAACCAAAGATTA pLKO.1 2557 CDS 100% 13.200 18.480 N RICTOR n/a
4 TRCN0000307119 CGTCGGAGTAACCAAAGATTA pLKO_005 2557 CDS 100% 13.200 18.480 N RICTOR n/a
5 TRCN0000074288 CCGCAGTTACTGGTACATGAA pLKO.1 5213 3UTR 100% 4.950 6.930 N RICTOR n/a
6 TRCN0000307122 CCGCAGTTACTGGTACATGAA pLKO_005 5213 3UTR 100% 4.950 6.930 N RICTOR n/a
7 TRCN0000074292 CCCTAATGAATATGGCTGCAT pLKO.1 1316 CDS 100% 2.640 3.696 N RICTOR n/a
8 TRCN0000296313 CCGATCATGGGCAGGTATTAT pLKO_005 840 CDS 100% 15.000 12.000 N RICTOR n/a
9 TRCN0000074290 GCACCCTCTATTGCTACAATT pLKO.1 3877 CDS 100% 13.200 9.240 N RICTOR n/a
10 TRCN0000289690 GCACCCTCTATTGCTACAATT pLKO_005 3877 CDS 100% 13.200 9.240 N RICTOR n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017009312.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13449 pDONR223 100% 14.4% 13.7% None (many diffs) n/a
2 TRCN0000477715 GGAATGATAATTCGAGGGAACCAG pLX_317 56% 14.4% 13.7% V5 (many diffs) n/a
3 ccsbBroad304_13449 pLX_304 83.7% 14.4% 1.8% V5 (not translated due to prior stop codon) (many diffs) n/a
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