Construct: ORF ccsbBroad304_13628
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004834.1_s304c1, BRDN0000400839
- Derived from:
- ccsbBroadEn_13628
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WASH3P (374666)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13628
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 100287171 | WASHC1 | WASH complex subunit 1 | NM_182905.5 | 97.8% | 96.3% | (many diffs) |
| 2 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XM_024447369.1 | 97.8% | 96.3% | (many diffs) |
| 3 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XM_011517662.3 | 96.6% | 85% | (many diffs) |
| 4 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XM_011517658.2 | 95% | 93.5% | (many diffs) |
| 5 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XM_011517659.2 | 94.6% | 83.5% | (many diffs) |
| 6 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XM_011517660.2 | 94.5% | 84.1% | (many diffs) |
| 7 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XM_011517665.2 | 90.9% | 87.3% | (many diffs) |
| 8 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XM_011517663.3 | 90.6% | 84.9% | (many diffs) |
| 9 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XM_017014169.1 | 90.6% | 84.9% | (many diffs) |
| 10 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XM_011517664.3 | 90.4% | 84.9% | (many diffs) |
| 11 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XM_017014170.1 | 90.4% | 84.9% | (many diffs) |
| 12 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XM_011517666.2 | 87.6% | 80.7% | (many diffs) |
| 13 | human | 102723897 | WASH9P | WAS protein family homolog ... | XR_001737556.1 | 77.4% | (many diffs) | |
| 14 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XM_017014171.1 | 77.2% | 51.5% | (many diffs) |
| 15 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XM_017014172.1 | 77.2% | 51.5% | (many diffs) |
| 16 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XR_002956737.1 | 75.8% | (many diffs) | |
| 17 | human | 653635 | WASH7P | WASP family homolog 7, pseu... | NR_024540.1 | 75% | (many diffs) | |
| 18 | human | 100288778 | WASH8P | WAS protein family homolog ... | NR_130745.1 | 72.1% | (many diffs) | |
| 19 | human | 375260 | WASH2P | WASP family homolog 2, pseu... | NR_024077.2 | 68% | (many diffs) | |
| 20 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XR_002956741.1 | 66.4% | (many diffs) | |
| 21 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XR_929142.2 | 64% | (many diffs) | |
| 22 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XM_024447370.1 | 55.3% | 54.4% | (many diffs) |
| 23 | human | 374666 | WASH3P | WASP family homolog 3, pseu... | NR_003659.2 | 52% | (many diffs) | |
| 24 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XM_017014173.1 | 49.5% | 41.2% | (many diffs) |
| 25 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XR_002956738.1 | 36.8% | (many diffs) | |
| 26 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XR_002956740.1 | 17.3% | (many diffs) | |
| 27 | human | 100287171 | WASHC1 | WASH complex subunit 1 | XR_002956739.1 | 17.2% | (many diffs) | |
| 28 | mouse | 68767 | Washc1 | WASH complex subunit 1 | NM_001037757.1 | 81.1% | 84.4% | (many diffs) |
| 29 | mouse | 68767 | Washc1 | WASH complex subunit 1 | NM_026833.1 | 81.1% | 84.4% | (many diffs) |
| 30 | mouse | 68767 | Washc1 | WASH complex subunit 1 | XM_006524859.1 | 81.1% | 84.4% | (many diffs) |
| 31 | mouse | 68767 | Washc1 | WASH complex subunit 1 | XM_006524860.3 | 81.1% | 84.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1470
- ORF length:
- 1404
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac tcctgtgagg atgcagcact ccctggcagg tcagacctat gccgtgcccc 121 tcatccagcc agacctgcgg cgagaggagg ccgtccagca gatggcggat gccctgcagt 181 acctgcagaa ggtctctgga gacatcttca gcaggatctc ccagcgggta gagcagagcc 241 ggagccaggt gcaggccatt ggagagaagg tctccttggc ccaggccaag attgagaaga 301 tcaagggcag caagaaggcc atcaaggtgt tctccagtgc caagtaccct gctccagagc 361 gcctgcagga atatggctcc atcttcacgg gcgcccagga ccctggcctg cagagacgct 421 cccgccacag gatccagagc aagcaccgcc ccctggacga gcgggccctg caggagaagc 481 tgaagtactt tcctgtgtgt gtgagcacca agccggagcc cgaggacgat gcagaagagg 541 gacttggggg tcttcccagc aacatcagct ctgtcagctc cttgctgctc ttcaacacca 601 ccgagaacct gtacaagaag tatgtcttcc tggaccccct ggctggtgct gtaacaaaga 661 cccatgtgat gctgggggca gagacagagg agaagctgtt tgatgccccc ttgtccatca 721 gcaagagaga gcagctggaa cagcaggtcc cagagaacta cttctatgtg ccagacctgg 781 gccaggtgcc tgagattgat gttccgtcct acctgcctga cctgcccggc attgccaacg 841 acctcatgta cagtgccgac ctgggccccg gcattgcccc ctctgcccct ggcaccattc 901 cggaactgcc caccttccac actgaggtag ccgagcctct caaggcagac ctacaagatg 961 gggtactaac agcaccacca ccacccccac cgcccccacc acctccccca gctcctgagg 1021 tgctggccag tgcatcccca ctcccaccct caaccgcggc ccctgtaggc caaggcgcca 1081 ggcaggacga cagcagcagc agcgcgtctc cttcagtcca gggagctccc agggaagtgg 1141 tcgacccctc cggtggccgg gccactctgc tagagtccat ccgccaagct gggggcatcg 1201 gcaaggccaa gctgcgcagc gtgaaggagc gaaagctgga gaagaagaag cagaaggagc 1261 aggagcaagt gagagccacg agccaaggtg gggacttgat gtcggatctc ttcaacaagc 1321 tggtcatgag gcgcaagggc atctctggga aaggacctgg ggctggtgag gggcccggag 1381 gagcctttgc ccgcgtgtca gactccatcc ctcctctgcc gccaccgcag cagccacagg 1441 cagaggagga cgaggacgac tgggaatcgt acccaacttt cttgtacaaa gtggttggta 1501 agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc 1561 ggtggcgcgt taagtcgaca atcaacctct ggatta