Construct: ORF ccsbBroad304_13680
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013262.1_s304c1, BRDN0000405105
- Derived from:
- ccsbBroadEn_13680
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NOMO3 (408050)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13680
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 283820 | NOMO2 | NODAL modulator 2 | NM_173614.2 | 54.2% | 54.2% | 1_1677del |
| 2 | human | 408050 | NOMO3 | NODAL modulator 3 | NM_001004067.4 | 54.2% | 54.2% | 1_1677del;2211T>C |
| 3 | human | 23420 | NOMO1 | NODAL modulator 1 | NM_014287.4 | 54% | 54% | (many diffs) |
| 4 | human | 283820 | NOMO2 | NODAL modulator 2 | NM_001004060.1 | 52.3% | 52.3% | 1_1677del;3666_3801delinsG |
| 5 | human | 408050 | NOMO3 | NODAL modulator 3 | XM_005255318.1 | 52.2% | 52.3% | 1_1677del;2211T>C;3666_3801delinsG |
| 6 | human | 102723728 | LOC102723728 | nodal modulator 3-like | XM_006720996.3 | 44.3% | 44.3% | 0_1ins1107 |
| 7 | mouse | 211548 | Nomo1 | nodal modulator 1 | NM_153057.4 | 47.2% | 51.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 2055
- ORF length:
- 1989
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca tgaggattgg tgctggaaga acaagagcct ggaggtggaa gtgctggagg 121 atgacgtgtc tgcagttgag ttcaggcaga cgggctacat gctgagatgt tccctgtctc 181 acgccatcac tctggaattt tatcaggatg gaaatgggcg tgagaatgtg gggatttata 241 acctctccaa aggagtcaac cgattctgcc tgtccaagcc tggtgtgtac aaagtgaccc 301 ctcgctcctg ccaccggttt gagcaagcgt tctacaccta tgacacgtct tcacctagta 361 tcttgacatt gacagccatt cgccaccatg tccttggaac tatcaccacc gacaaaatga 421 tggatgtcac tgtgactatc aagtcttcca tcgacagtga acccgccttg gtcttaggcc 481 ctctgaagtc tgtgcaggag ctgcggaggg agcagcagct ggctgagatc gaggcccgca 541 ggcaggagag ggagaaaaac ggcaatgagg aaggtgaaga aagaatgacc aagcctcccg 601 tgcaggagat ggtagatgag ttacaaggcc ccttctcgta tgatttctct tactgggcgc 661 ggtctggaga gaaaatcact gttacaccgt catctaaaga gctgctcttt tatccccctt 721 caatggaagc cgttgtcagt ggagaaagct gcccagggaa gctgatcgag atccacggga 781 aggcaggcct gtttttagaa ggccagatcc accccgagtt ggaaggagtc gagattgtca 841 tcagtgaaaa gggggcaagt tcaccgctga tcacagtctt tactgatgac aaaggtgcct 901 acagtgttgg ccccctgcac agtgacctgg agtacacggt gacctcacag aaggagggct 961 atgttctgac tgcagtggaa ggaaccatcg gagacttcaa ggcctatgcc ctggcaggcg 1021 taagctttga gataaaagct gaggatgacc agcccctccc gggagtcctc ttatccctga 1081 gtggtggcct gtttcgttcc aacctcttga cccaggacaa cggcattctg acattctcaa 1141 acctgagccc tggccagtat tacttcaaac ccatgatgaa ggagttccgg tttgagccat 1201 cctcacagat gatcgaggtg caggaaggcc agaacctgaa gatcaccatc acggggtacc 1261 gaaccgctta cagttgctat ggcacagtgt cttccttaaa cggagagccc gaacaagggg 1321 ttgccatgga agcggtgggc cagaacgact gcagcattta cggagaagac accgtgacag 1381 acgaagaggg caagttcaga ttacgtggat tgctgccggg atgtgtgtac cacgttcagc 1441 tcaaggcaga aggcaacgac cacattgagc gggcgctccc ccaccatagg gtgattgagg 1501 ttgggaataa tgacatcgat gatgtaaaca tcatagtttt ccggcagatt aatcaatttg 1561 atttaagtgg aaatgtgatc acttcctctg aataccttcc tacgttatgg gtcaagcttt 1621 acaaaagcga aaacctcgac aatccaatcc agacagtttc ccttggccag tccctgttct 1681 tccatttccc cccactgctc agagatggcg agaactatgt tgtgcttctg gactccacac 1741 tccccagatc ccagtatgac tacatcttgc ctcaagtttc tttcaccgca gtgggctacc 1801 ataaacacat caccttgatt tttaatccca cgaggaagct gcctgaacag gacatcgcac 1861 aaggatccta cattgccctg ccattgacgc tgctggttct gctggccggt tacaaccatg 1921 acaagctcat tcctttgctg ctgcagttga caagccggct acagggagtc ggcgcgctcg 1981 gccaggcagc ctctgacaat agcggcccag aagatgcaaa gagacaagcc aagaaacaga 2041 agacaaggcg gacgtgccca actttcttgt acaaagtggt tggtaagcct atccctaacc 2101 ctctcctcgg tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt 2161 cgacaatcaa cctctggatt a