Construct: ORF ccsbBroad304_13695
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002908.1_s304c1, BRDN0000404781
- Derived from:
- ccsbBroadEn_13695
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MTHFD2L (441024)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13695
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | NM_001351314.2 | 31.8% | 31.8% | 1_450del |
| 2 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | NM_001004346.4 | 24.2% | 24.2% | 1_657del |
| 3 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | NM_001144978.2 | 20.1% | 20.1% | 1_831del |
| 4 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | XM_005265690.2 | 16.3% | 11% | 1_657del;758_831del;885_886ins56 |
| 5 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | XM_017008225.1 | 16.3% | 11% | 1_657del;758_831del;885_886ins56 |
| 6 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | XM_017008226.1 | 16.3% | 11% | 1_657del;758_831del;885_886ins56 |
| 7 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | XM_017008224.1 | 14.8% | 10% | 1_753del;854_927del;981_982ins56 |
| 8 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | XM_017008218.2 | 10.8% | 7.3% | 1_1140del;1241_1314del;1368_1369ins56 |
| 9 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | NR_147097.2 | 8.4% | 1_984del;1195_2487del | |
| 10 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | XR_001741222.2 | 7.4% | 1_1330del;1541_2833del | |
| 11 | human | 441024 | MTHFD2L | methylenetetrahydrofolate d... | XR_001741224.2 | 7.3% | 1_1336del;1547_2839del | |
| 12 | mouse | 665563 | Mthfd2l | methylenetetrahydrofolate d... | XM_006535178.3 | 21.5% | 22.9% | (many diffs) |
| 13 | mouse | 665563 | Mthfd2l | methylenetetrahydrofolate d... | NM_026788.1 | 17.7% | 18.9% | (many diffs) |
| 14 | mouse | 665563 | Mthfd2l | methylenetetrahydrofolate d... | XM_006535176.3 | 16.3% | 17.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 276
- ORF length:
- 210
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt taaagaaggt gctgctgtaa ttgatgtggg tatcaactat gtccacgatc 121 cagtgacagg aaagacaaaa ttagttggag atgtggactt cgaagctgtt aaaaagaaag 181 ctggctttat cactccagtt ccaggaggtg tgggacccat gacagtggca atgcttctga 241 agaacaccct tctggcagct aaaaaaatca tttactaccc aactttcttg tacaaagtgg 301 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 361 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta