Transcript: Mouse XM_006535176.3

PREDICTED: Mus musculus methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2-like (Mthfd2l), transcript variant X1, mRNA.

Source:
NCBI, updated 2016-06-22
Taxon:
Mus musculus (mouse)
Gene:
Mthfd2l (665563)
Length:
2297
CDS:
93..1193

Additional Resources:

NCBI RefSeq record:
XM_006535176.3
NBCI Gene record:
Mthfd2l (665563)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_006535176.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000265805 CCAAGAGTCAGCGGTATATTA pLKO_005 561 CDS 100% 15.000 21.000 N Mthfd2l n/a
2 TRCN0000265821 GGGTATTCCCAGGTTGATTAC pLKO_005 953 CDS 100% 10.800 15.120 N Mthfd2l n/a
3 TRCN0000256659 GAAATGTGTGTGACCATTATT pLKO_005 2090 3UTR 100% 15.000 10.500 N Mthfd2l n/a
4 TRCN0000256660 TCTGCAGTGAGCTCATCATAA pLKO_005 481 CDS 100% 13.200 9.240 N Mthfd2l n/a
5 TRCN0000256658 TGATGTGGGTATCAACTATAT pLKO_005 1007 CDS 100% 13.200 7.920 N Mthfd2l n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_006535176.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13694 pDONR223 100% 33.6% 35.5% None (many diffs) n/a
2 ccsbBroad304_13694 pLX_304 0% 33.6% 35.5% V5 (many diffs) n/a
3 TRCN0000472987 ATCTCCGTTAAATGTTGACGAACG pLX_317 86.2% 33.6% 35.5% V5 (many diffs) n/a
4 ccsbBroadEn_13695 pDONR223 100% 16.3% 17.4% None (many diffs) n/a
5 ccsbBroad304_13695 pLX_304 0% 16.3% 17.4% V5 (many diffs) n/a
6 TRCN0000467739 AGTTTAGGAATAACAATACATCTC pLX_317 100% 16.3% 17.4% V5 (many diffs) n/a
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