Construct: ORF ccsbBroad304_13729
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004843.1_s304c1, BRDN0000400983
- Derived from:
- ccsbBroadEn_13729
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RPL23AP64 (649946)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13729
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 649946 | RPL23AP64 | ribosomal protein L23a pseu... | NR_003040.2 | 36.3% | (many diffs) | |
| 2 | human | 6147 | RPL23A | ribosomal protein L23a | NM_000984.6 | 35% | 32.3% | (many diffs) |
| 3 | human | 56969 | RPL23AP32 | ribosomal protein L23a pseu... | NR_002229.2 | 31.1% | (many diffs) | |
| 4 | human | 285830 | HLA-F-AS1 | HLA-F antisense RNA 1 | NR_026973.1 | 30.7% | (many diffs) | |
| 5 | human | 118433 | RPL23AP7 | ribosomal protein L23a pseu... | NR_000029.3 | 17.5% | (many diffs) | |
| 6 | human | 118433 | RPL23AP7 | ribosomal protein L23a pseu... | NR_024529.1 | 15.5% | (many diffs) | |
| 7 | human | 284942 | RPL23AP82 | ribosomal protein L23a pseu... | NR_026982.1 | 14.9% | (many diffs) | |
| 8 | human | 118433 | RPL23AP7 | ribosomal protein L23a pseu... | NR_024528.1 | 14.5% | (many diffs) | |
| 9 | human | 284942 | RPL23AP82 | ribosomal protein L23a pseu... | NR_026981.1 | 14.2% | (many diffs) | |
| 10 | human | 285830 | HLA-F-AS1 | HLA-F antisense RNA 1 | NR_026972.1 | 11.2% | (many diffs) | |
| 11 | human | 388574 | RPL23AP87 | ribosomal protein L23a pseu... | NR_029406.1 | 9.5% | (many diffs) | |
| 12 | human | 644128 | RPL23AP53 | ribosomal protein L23a pseu... | NR_003572.2 | 3.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 309
- ORF length:
- 243
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa gaagacagaa gacaacaacg cacttgtgtt cactgtgaat gttaaagcca 121 acaagcacca gaagaagctc tatgacactg atgtggccaa ggtcaacacc ctgattcggc 181 ctgatggaga gaaggaacat gttcgactgg ctcctgatta caatgctttg gatgttgcca 241 acaaaatagg gatcatctca actgagtcca gttggctaat tctaaatata tgtatatctt 301 ttcaccatta cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 361 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 421 tcaacctctg gatta