Construct: ORF ccsbBroad304_13809
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004748.1_s304c1, BRDN0000409481
- Derived from:
- ccsbBroadEn_13809
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ADD1 (118)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13809
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 118 | ADD1 | adducin 1 | NM_001354757.2 | 63.3% | 62.1% | 19G>T;1174_1315del;1343_1893del |
2 | human | 118 | ADD1 | adducin 1 | NM_001354758.1 | 63.3% | 62.1% | 19G>T;1174_1315del;1343_1893del |
3 | human | 118 | ADD1 | adducin 1 | NM_014190.4 | 63.3% | 62.1% | 19G>T;1174_1315del;1343_1893del |
4 | human | 118 | ADD1 | adducin 1 | XM_017007709.2 | 63.3% | 62.1% | 19G>T;1174_1315del;1343_1893del |
5 | human | 118 | ADD1 | adducin 1 | NM_001286645.1 | 60.3% | 59.2% | 19G>T;1174_1315del;1343_1986del |
6 | human | 118 | ADD1 | adducin 1 | NM_001354759.2 | 60.3% | 59.2% | 19G>T;1174_1315del;1343_1986del |
7 | human | 118 | ADD1 | adducin 1 | NM_176801.2 | 60.3% | 59.2% | 19G>T;1174_1315del;1343_1986del |
8 | human | 118 | ADD1 | adducin 1 | XM_024453887.1 | 60.3% | 59.2% | 19G>T;1174_1315del;1343_1986del |
9 | human | 118 | ADD1 | adducin 1 | NM_001354762.2 | 57.6% | 56.5% | 19G>T;1174_1315del;1343_2079del |
10 | human | 118 | ADD1 | adducin 1 | NM_001119.5 | 54.2% | 53.1% | 19G>T;1174_1315del;1343_2211del |
11 | human | 118 | ADD1 | adducin 1 | NM_001354754.2 | 54.2% | 53.1% | 19G>T;1174_1315del;1343_2211del |
12 | human | 118 | ADD1 | adducin 1 | NM_001354755.1 | 54.2% | 53.1% | 19G>T;1174_1315del;1343_2211del |
13 | human | 118 | ADD1 | adducin 1 | XM_017007706.2 | 54.2% | 53.1% | 19G>T;1174_1315del;1343_2211del |
14 | human | 118 | ADD1 | adducin 1 | NM_001354756.2 | 52% | 51% | 19G>T;1174_1315del;1343_2304del |
15 | human | 118 | ADD1 | adducin 1 | NM_014189.3 | 52% | 51% | 19G>T;1174_1315del;1343_2304del |
16 | human | 118 | ADD1 | adducin 1 | XM_017007704.1 | 52% | 51% | 19G>T;1174_1315del;1343_2304del |
17 | human | 118 | ADD1 | adducin 1 | XM_017007705.1 | 52% | 51% | 19G>T;1174_1315del;1343_2304del |
18 | human | 118 | ADD1 | adducin 1 | NM_001354761.2 | 50% | 49% | 19G>T;1174_1315del;1343_2397del |
19 | human | 118 | ADD1 | adducin 1 | XM_005247934.2 | 50% | 49% | 19G>T;1174_1315del;1343_2397del |
20 | human | 118 | ADD1 | adducin 1 | XM_017007703.1 | 50% | 49% | 19G>T;1174_1315del;1343_2397del |
21 | human | 118 | ADD1 | adducin 1 | XR_002959706.1 | 44.3% | (many diffs) | |
22 | human | 118 | ADD1 | adducin 1 | XR_002959707.1 | 31.4% | (many diffs) | |
23 | mouse | 11518 | Add1 | adducin 1 (alpha) | NM_001331081.1 | 56.7% | 58.9% | (many diffs) |
24 | mouse | 11518 | Add1 | adducin 1 (alpha) | XM_006503681.2 | 56.7% | 58.9% | (many diffs) |
25 | mouse | 11518 | Add1 | adducin 1 (alpha) | NM_001331087.1 | 56.6% | 58.9% | (many diffs) |
26 | mouse | 11518 | Add1 | adducin 1 (alpha) | NM_013457.4 | 56.6% | 58.9% | (many diffs) |
27 | mouse | 11518 | Add1 | adducin 1 (alpha) | XM_017320615.1 | 56.6% | 58.9% | (many diffs) |
28 | mouse | 11518 | Add1 | adducin 1 (alpha) | XM_006503680.2 | 54.2% | 56.4% | (many diffs) |
29 | mouse | 11518 | Add1 | adducin 1 (alpha) | NM_001331086.1 | 54.2% | 56.3% | (many diffs) |
30 | mouse | 11518 | Add1 | adducin 1 (alpha) | XM_006503678.3 | 54.1% | 56.2% | (many diffs) |
31 | mouse | 11518 | Add1 | adducin 1 (alpha) | XM_006503679.2 | 54.1% | 56.2% | (many diffs) |
32 | mouse | 11518 | Add1 | adducin 1 (alpha) | NM_001102444.2 | 54% | 56.1% | (many diffs) |
33 | mouse | 11518 | Add1 | adducin 1 (alpha) | XM_006503677.2 | 54% | 56.1% | (many diffs) |
34 | mouse | 11518 | Add1 | adducin 1 (alpha) | XM_006503675.3 | 51.8% | 53.9% | (many diffs) |
35 | mouse | 11518 | Add1 | adducin 1 (alpha) | XM_006503676.2 | 51.8% | 53.9% | (many diffs) |
36 | mouse | 11518 | Add1 | adducin 1 (alpha) | NM_001331085.1 | 51.7% | 53.8% | (many diffs) |
37 | mouse | 11518 | Add1 | adducin 1 (alpha) | XM_006503674.2 | 51.7% | 53.8% | (many diffs) |
38 | mouse | 11518 | Add1 | adducin 1 (alpha) | NM_001024458.4 | 48.7% | 50.7% | (many diffs) |
39 | mouse | 11518 | Add1 | adducin 1 (alpha) | NM_001331080.1 | 48.7% | 50.7% | (many diffs) |
40 | mouse | 11518 | Add1 | adducin 1 (alpha) | NM_001331084.1 | 46.9% | 48.8% | (many diffs) |
41 | mouse | 11518 | Add1 | adducin 1 (alpha) | NM_001331083.1 | 46.7% | 48.7% | (many diffs) |
42 | mouse | 11518 | Add1 | adducin 1 (alpha) | XM_006503670.3 | 45.9% | 47.9% | (many diffs) |
43 | mouse | 11518 | Add1 | adducin 1 (alpha) | NM_001331082.1 | 45% | 46.9% | (many diffs) |
44 | mouse | 11518 | Add1 | adducin 1 (alpha) | XM_006503669.3 | 45% | 46.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1266
- ORF length:
- 1200
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa tggtgattct cgttctgcgg tggtgacctc accacccccg accacagccc 121 ctcacaagga gaggtacttc gaccgagtag atgagaacaa cccagagtac ttgagggaga 181 ggaacatggc accagacctt cgccaggact tcaacatgat ggagcaaaag aagagggtgt 241 ccatgattct gcaaagccct gctttctgtg aagaattgga atcaatgata caggagcaat 301 ttaagaaggg gaagaacccc acaggcctat tggcattaca gcagattgca gattttatga 361 ccacgaatgt accaaatgtc tacccagcag ctccgcaagg agggatggct gccttaaaca 421 tgagtcttgg tatggtgact cctgtgaacg atcttagagg atctgattct attgcgtatg 481 acaaaggaga gaagttatta cggtgtaaat tggcagcgtt ttatagacta gcagatctct 541 ttgggtggtc tcagcttatc tacaatcata tcacaaccag agtgaactcc gagcaggaac 601 acttcctcat tgtccctttt gggcttcttt acagtgaagt gactgcatcc agtttggtta 661 agatcaatct acaaggagat atagtagatc gtggaagcac taatctggga gtgaatcagg 721 ccggcttcac cttacactct gcaatttatg ctgcacgccc ggacgtgaag tgcgtcgtgc 781 acattcacac cccagcaggg gctgcggtct ctgcaatgaa atgtggcctc ttgccaatct 841 ccccggaggc gctttccctt ggagaagtgg cttatcatga ctaccatggc attctggttg 901 atgaagagga aaaagttttg attcagaaaa atctggggcc taaaagcaag gttcttattc 961 tccggaacca tgggctcgtg tcagttggag agagcgttga ggaggccttc tattacatcc 1021 ataaccttgt ggttgcctgt gagatccagg ttcgaactct ggccagtgca ggaggaccag 1081 acaacttagt cctgctgaat cctgagaagt acaaagccaa gtcccgttcc ccagggtctc 1141 cggtagggga aggcactgga tcgcctccca agtggcagat tggtgagcag gaatttgaag 1201 ccctcatgcg gatgctcgat aatctgggct acagaactag cagcagcggg agaagacaag 1261 atggctgccc aactttcttg tacaaagtgg ttggtaagcc tatccctaac cctctcctcg 1321 gtctcgattc tacgtagtaa tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca 1381 acctctggat ta