Construct: ORF ccsbBroad304_13811
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011438.1_s304c1, BRDN0000407405
- Derived from:
- ccsbBroadEn_13811
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ALDH3B1 (221)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13811
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 221 | ALDH3B1 | aldehyde dehydrogenase 3 fa... | NM_001290058.2 | 58.4% | 5.9% | (many diffs) |
| 2 | human | 221 | ALDH3B1 | aldehyde dehydrogenase 3 fa... | NM_000694.4 | 47.9% | 4.5% | (many diffs) |
| 3 | human | 221 | ALDH3B1 | aldehyde dehydrogenase 3 fa... | NM_001161473.3 | 47.9% | 4.5% | (many diffs) |
| 4 | human | 221 | ALDH3B1 | aldehyde dehydrogenase 3 fa... | NM_001030010.3 | 40% | 1.2% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 309
- ORF length:
- 243
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga ccccttgggg acacgctgcg gcgactgcgg gaggccttcc acgcggggcg 121 cacacggcca gctgagttcc gggctgcgca gctccaaggc ctgggccgct tcctgcaaga 181 aaacaagcag cttctgcacg acgcactggc ccaggacctg cacaagtcag ccttcgagtc 241 ggaggtgtct gaggttgcca tcagccaggg cgaggtcacc ctggccctca ggaacctccg 301 ggcctggatg aaggacgagc gtgtgcccaa gaacctggcc acgcagctgg actccgcctt 361 catccggaag gagccctttg gcctggtcct catcattgcg ccctggaact atccgctgaa 421 cctgacgctg gtgcccctcg tgggagccct cgctgcaggg aactgtgtgg tgctgaagcc 481 atcggagatt agcaagaacg tcgagaagat cctggccgag gtgctgcccc aatacgtgga 541 ccagagctgc tttgctgtgg tgctgggcgg gccccaggag acggggcagc tgctagagca 601 caggttcgac tacatcttct tcacagggag ccctcgtgtg ggcaagattg ttatgactgc 661 tgccgccaag cacccctcaa agcagcgcct gcctcctccc tcctgggtct tccctctccc 721 tgcctcagcc tcctccctca gccgctccca accatgccca actttcttgt acaaagtggt 781 tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat gagctagcgc 841 taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a