Construct: ORF ccsbBroad304_13853
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004438.1_s304c1, BRDN0000399182
- Derived from:
- ccsbBroadEn_13853
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- EPB41L1 (2036)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13853
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | NM_001258329.1 | 99.9% | .5% | 12delG |
| 2 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_017027716.1 | 99.9% | .5% | 12delG |
| 3 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_017027717.2 | 99.9% | .5% | 12delG |
| 4 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | NM_012156.2 | 99.8% | .5% | 12delG;2182_2184delCAG |
| 5 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_011528681.1 | 98.5% | .5% | 12delG;1447_1448ins36 |
| 6 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_011528682.1 | 98.4% | .5% | 12delG;1298_1299insAGC;1444_1445ins36 |
| 7 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_017027715.1 | 98.3% | .5% | (many diffs) |
| 8 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_011528683.1 | 96.8% | .5% | 12delG;2183_2184ins81 |
| 9 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_017027712.2 | 94.2% | .4% | (many diffs) |
| 10 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_017027713.2 | 92.9% | .4% | (many diffs) |
| 11 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_017027714.2 | 89.9% | .4% | (many diffs) |
| 12 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | NM_001258331.2 | 88.5% | .3% | 0_1ins185;1261_1262ins36;1961_1962ins81 |
| 13 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | NM_177996.2 | 88.5% | .3% | 0_1ins185;1261_1262ins36;1961_1962ins81 |
| 14 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_011528685.1 | 84.2% | .6% | 12delG;1664_1665ins411;1771_1773delCAG |
| 15 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_017027720.1 | 79.9% | .7% | (many diffs) |
| 16 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_017027718.2 | 78.8% | .5% | (many diffs) |
| 17 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | NM_001258330.1 | 77.5% | .5% | (many diffs) |
| 18 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_017027719.2 | 77.5% | .5% | (many diffs) |
| 19 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_024451854.1 | 74.3% | .6% | 0_1ins185;1478_1479ins411;1586_1587ins81 |
| 20 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_011528671.1 | 56.3% | .3% | 12delG;1668_3704del;4219_4221delCAG |
| 21 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_011528673.1 | 56.3% | .3% | 12delG;1668_3704del;4219_4221delCAG |
| 22 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_024451851.1 | 53.8% | .1% | (many diffs) |
| 23 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_011528667.1 | 53.5% | .2% | (many diffs) |
| 24 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_011528677.1 | 52.4% | .2% | 0_1ins185;1482_3518del;4033_4035delCAG |
| 25 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_017027711.1 | 52.4% | .2% | 0_1ins185;1482_3518del;4033_4035delCAG |
| 26 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_024451852.1 | 52.4% | .2% | 0_1ins185;1482_3518del;4033_4035delCAG |
| 27 | human | 2036 | EPB41L1 | erythrocyte membrane protei... | XM_024451853.1 | 52.4% | .2% | 0_1ins185;1482_3518del;4033_4035delCAG |
| 28 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | NM_001006664.3 | 90.8% | .5% | (many diffs) |
| 29 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | NM_001291120.1 | 90.8% | .5% | (many diffs) |
| 30 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | NM_013510.4 | 90.8% | .5% | (many diffs) |
| 31 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | XM_017315393.1 | 90.7% | .5% | (many diffs) |
| 32 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | XM_017315394.1 | 90% | .5% | (many diffs) |
| 33 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | NM_001291121.1 | 89.5% | .5% | (many diffs) |
| 34 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | XM_011239281.1 | 76.8% | .6% | (many diffs) |
| 35 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | NM_001291122.1 | 75.6% | .6% | (many diffs) |
| 36 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | NM_001291123.1 | 75.4% | .6% | (many diffs) |
| 37 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | XM_006498711.3 | 50.2% | .3% | (many diffs) |
| 38 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | XM_006498713.3 | 50.2% | .3% | (many diffs) |
| 39 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | XM_006498714.3 | 50.2% | .3% | (many diffs) |
| 40 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | XM_011239280.2 | 50.2% | .3% | (many diffs) |
| 41 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | XM_017315383.1 | 50.2% | .3% | (many diffs) |
| 42 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | XM_011239275.2 | 50.1% | .3% | (many diffs) |
| 43 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | XM_006498708.3 | 49.8% | .3% | (many diffs) |
| 44 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | XM_011239276.1 | 49.7% | .3% | (many diffs) |
| 45 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | XM_011239277.1 | 49.5% | .3% | (many diffs) |
| 46 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | XM_011239278.1 | 49% | .3% | (many diffs) |
| 47 | mouse | 13821 | Epb41l1 | erythrocyte membrane protei... | XM_011239279.1 | 46.7% | .3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 96
- ORF length:
- 30
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgac aacagaacag gccccgactc tgaggtgaag aaagctcagg aggaggcccc 121 gcagcagccc gaggctgctg ccgctgtgac cacccctgtg acccctgcag gccacggcca 181 cccagaggcc aactccaatg agaagcatcc atcccagcag gacacgcggc ctgctgaaca 241 gagcctagac atggaggaga aggactacag tgaggccgat ggcctttcgg agaggaccac 301 gcccagcaag gcccagaaat cgccccagaa gattgccaag aaatacaaga gtgccatctg 361 ccgggtcact ctgcttgatg cctcggagta tgagtgtgag gtggagaaac atggccgggg 421 ccaggtgctg tttgacctgg tctgtgaaca cctcaacctc ctagagaagg actacttcgg 481 cctgaccttc tgtgatgctg acagccagaa gaactggctg gacccctcca aggagatcaa 541 gaagcagatc cggagtagcc cctggaattt tgccttcaca gtcaagttct acccgcctga 601 tcctgcccag ctgacagaag acatcacaag atactacctg tgcctgcagc tgcgggcaga 661 catcatcacg ggccggctgc catgctcctt tgtcacgcat gccctactgg gctcctacgc 721 tgtgcaggct gagctgggtg actatgatgc tgaggagcat gtgggcaact atgtcagcga 781 gctccgcttc gcccctaacc agacccggga gctggaggag aggatcatgg agctgcataa 841 gacatatagg gggatgaccc cgggagaagc agaaatccac ttcttagaga atgccaagaa 901 gctttccatg tacggagtag acctgcacca tgccaaggac tctgagggca tcgacatcat 961 gttaggcgtt tgtgccaatg gcctgctcat ctaccgggac cggctgagaa tcaaccgctt 1021 tgcctggccc aagatcctca agatctccta caagaggagt aacttctata tcaagatccg 1081 gcctggggag tatgagcaat ttgagagcac aattggcttt aagctcccaa accaccggtc 1141 agccaagaga ctgtggaagg tctgcatcga gcatcataca ttcttccggc tggtgtcccc 1201 tgagccccca cccaagggct tcctggtgat gggctccaag ttccggtaca gtgggaggac 1261 ccaggcacag actcgccagg ccagcgccct cattgaccgg cctgcaccct tctttgagcg 1321 ttcttccagc aaacggtaca ccatgtcccg cagccttgat ggagcagagt tctcccgccc 1381 agcctcggtc agcgagaacc atgatgcagg gcctgacggt gacaagcggg atgaggatgg 1441 cgagtctggg gggcaacggt cagaggctga ggagggagag gtcaggactc caaccaagat 1501 caaggagcta aagccggagc aggaaaccac gccgagacac aagcaggagt tcttagacaa 1561 gccagaagat gtcttgctga agcaccaggc cagcatcaat gagctcaaaa ggaccctgaa 1621 ggagcccaac agcaaactca tccaccggga tcgagactgg gaacgggagc gcaggctgcc 1681 ctcctccccc gcctccccct cccccaaggg cacccctgag aaagccaatg agagagcagg 1741 gctgagggag ggctccgagg agaaagtcaa accaccacgt ccccgggccc cagagagtga 1801 cacaggcgat gaggaccagg accaggagag ggacacggtg ttcctgaagg acaaccacct 1861 ggccattgag cgcaagtgct ccagcatcac ggtcagctct acgtctagcc tggaggctga 1921 ggtggacttc acggtcattg gtgactacca tggcagcgcc ttcgaagact tctcccgcag 1981 cctgcctgag ctcgaccggg acaaaagcga ctcggacact gagggcctgc tgttctcccg 2041 ggatctcaac aagggggccc ccagccagga tgatgagtct gggggcattg aggacagccc 2101 ggatcgaggg gcctgctcca ccccggatat gccccagttt gagcccgtga aaacagaaac 2161 catgactgtc agcagtctgg ccattagaaa gaagattgag ccggaggccg tactgcagac 2221 cagagtctcc gctatggata acacccaggt tgatgggagt gcctcagtgg ggagggagtt 2281 catagcaacc actccctcca tcaccacgga gaccatatcg accaccatgg agaacagtct 2341 caagtccggg aagggggcag ctgccatgat cccaggccca cagacggtgg ccacggaaat 2401 ccgttctctt tctccgatca tcgggaaaga tgtcctcacc agcacctacg gcgccactgc 2461 ggaaaccctc tcaacctcca ccaccaccca tgtcaccaaa actgtgaaag gagggttttc 2521 tgagacaagg atcgagaagc gaatcatcat tactggggat gaagatgtcg atcaagacca 2581 ggccctggct ttggccatca aggaggccaa actgcagcat cctgatatgc tggtaaccaa 2641 agctgtcgta tacagagaaa cagacccatc cccagaggag agggacaaga agccacagga 2701 atcctgccca actttcttgt acaaagtggt tggtaagcct atccctaacc ctctcctcgg 2761 tctcgattct acgtagtaat gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa 2821 cctctggatt a