Transcript: Human XM_024451854.1

PREDICTED: Homo sapiens erythrocyte membrane protein band 4.1 like 1 (EPB41L1), transcript variant X22, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
EPB41L1 (2036)
Length:
5588
CDS:
165..2129

Additional Resources:

NCBI RefSeq record:
XM_024451854.1
NBCI Gene record:
EPB41L1 (2036)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_024451854.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000307331 TCGAGAAGCGAATCATCATTA pLKO_005 1954 CDS 100% 13.200 18.480 N EPB41L1 n/a
2 TRCN0000294455 GGTAACCAAAGCTGTCGTATA pLKO_005 2054 CDS 100% 10.800 15.120 N EPB41L1 n/a
3 TRCN0000116473 CCGTTCTCTTTCTCCGATCAT pLKO.1 1823 CDS 100% 4.950 6.930 N EPB41L1 n/a
4 TRCN0000287064 CCGTTCTCTTTCTCCGATCAT pLKO_005 1823 CDS 100% 4.950 6.930 N EPB41L1 n/a
5 TRCN0000116475 CCAGAAGATTGCCAAGAAATA pLKO.1 239 CDS 100% 13.200 9.240 N EPB41L1 n/a
6 TRCN0000287065 CCAGAAGATTGCCAAGAAATA pLKO_005 239 CDS 100% 13.200 9.240 N EPB41L1 n/a
7 TRCN0000116474 CCACGGAAATCCGTTCTCTTT pLKO.1 1813 CDS 100% 4.950 3.465 N EPB41L1 n/a
8 TRCN0000116472 CCCACTTGAATGCAAAGGAAA pLKO.1 2606 3UTR 100% 4.950 3.465 N EPB41L1 n/a
9 TRCN0000286998 CCCACTTGAATGCAAAGGAAA pLKO_005 2606 3UTR 100% 4.950 3.465 N EPB41L1 n/a
10 TRCN0000116476 CCAACCAAGATCAAGGAGCTA pLKO.1 1404 CDS 100% 2.640 1.848 N EPB41L1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_024451854.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_00505 pDONR223 100% 81.1% 81.1% None 1262_1297del;1478_1479ins411 n/a
2 ccsbBroad304_00505 pLX_304 0% 81.1% 81.1% V5 1262_1297del;1478_1479ins411 n/a
3 TRCN0000472080 CGCAGGTTGCACAGAATGCTCGGT pLX_317 10.9% 81.1% 81.1% V5 1262_1297del;1478_1479ins411 n/a
4 ccsbBroadEn_13853 pDONR223 100% 74.3% .6% None 0_1ins185;1478_1479ins411;1586_1587ins81 n/a
5 ccsbBroad304_13853 pLX_304 0% 74.3% .6% V5 (not translated due to prior stop codon) 0_1ins185;1478_1479ins411;1586_1587ins81 n/a
6 TRCN0000473176 TATGTAAATCCTCTGCTGACGAAA pLX_317 1.2% 74.3% .6% V5 (not translated due to prior stop codon) 0_1ins185;1478_1479ins411;1586_1587ins81 n/a
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