Construct: ORF ccsbBroad304_13856

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF008553.1_s304c1, BRDN0000461744
Derived from:
ccsbBroadEn_13856
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
EPS15 (2060)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13856
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 2060 EPS15 epidermal growth factor rec... XM_017000617.2 91.9% 91.5% (many diffs)
2 human 2060 EPS15 epidermal growth factor rec... XM_017000618.2 88.3% 87.9% (many diffs)
3 human 2060 EPS15 epidermal growth factor rec... XM_017000616.2 87.9% 87.6% (many diffs)
4 human 2060 EPS15 epidermal growth factor rec... NM_001981.3 84.9% 84.5% (many diffs)
5 human 2060 EPS15 epidermal growth factor rec... XM_005270618.3 81.6% 81.2% (many diffs)
6 human 2060 EPS15 epidermal growth factor rec... XM_017000615.2 81.5% 81.2% (many diffs)
7 human 2060 EPS15 epidermal growth factor rec... XM_017000619.2 63.9% 63.6% (many diffs)
8 human 2060 EPS15 epidermal growth factor rec... XM_017000620.2 58.4% 58% (many diffs)
9 human 2060 EPS15 epidermal growth factor rec... NM_001159969.2 48.7% 46.3% (many diffs)
10 mouse 13858 Eps15 epidermal growth factor rec... NM_007943.3 76% 76.1% (many diffs)
11 mouse 13858 Eps15 epidermal growth factor rec... XM_006502738.2 73.1% 73.2% (many diffs)
12 mouse 13858 Eps15 epidermal growth factor rec... XM_006502739.3 70% 69.9% (many diffs)
13 mouse 13858 Eps15 epidermal growth factor rec... XM_017319956.1 65.1% 65.1% (many diffs)
14 mouse 13858 Eps15 epidermal growth factor rec... XM_006502740.2 62.6% 62.6% (many diffs)
15 mouse 13858 Eps15 epidermal growth factor rec... NM_001159964.1 42.9% 40.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2343
ORF length:
2277
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc tgcggcggcc cagctctctc tgacacagtt atcaagtggg aatcctgtat
121atgaaaaata ctatagacag gttgatacag gcaatactgg aagggtgttg gcttctgatg
181ctgctgcttt cctgaaaaaa tcagggcttc cagacttgat acttggaaag atttgggatt
241tagccgacac agatggcaaa ggtatcctga acaaacaaga attctttgtt gctttgcgtc
301ttgtggcatg tgcccagaat ggattggaag tttcactaag tagtttgaac ctggctgttc
361ctccaccaag atttcatgat accagtagtc ctttgctaat cagtggaacc tctgcagctg
421agctcccatg ggctgtaaaa cctgaagata aggccaaata tgatgcaata tttgatagtt
481taagcccagt gaatggattt ctgtctggtg ataaagtgaa accagtgttg ctcaactcta
541agttacctgt ggatatcctt ggaagagttt gggagttgag tgatattgac catgatggaa
601tgcttgacag agatgagttt gcagttgcta tgtttttggt atactgtgca ctggagaaag
661aacctgtgcc aatgtccttg cctccagcct tggtgccacc atctaagaga aaaacgtggg
721ttgtatcccc tgcagaaaaa gctaaatatg atgaaatctt cctgaaaact gataaagata
781tggacggatt tgtgtctgga ttggaggtcc gtgaaatatt cttgaaaaca ggtttacctt
841ctaccttact agcccatata tggtcattat gcgacacaaa ggactgtggg aagctttcaa
901aggatcagtt tgccttggct tttcacttaa tcagtcagaa gttaatcaag ggcattgatc
961ctcctcacgt tcttactcct gaaatgattc caccatcaga cagggccagt ttacaaaaga
1021acatcatagg atcaagtcct gttgcagatt tctctgctat taaggaacta gatactctta
1081acaatgaaat agttgaccta cagagggaaa agaataatgt ggaacaggac cttaaggaga
1141aggaagatac tattaaacag aggacaagtg aggttcagga tcttcaagat gaagttcaaa
1201gggagaatac taatctgcaa aaactacagg cccagaaaca gcaggtacag gaactccttg
1261atgaactgga tgagcagaaa gcccagctgg aggagcaact caaggaagtc agaaagaaat
1321gtgctgagga ggcccaactg gcaagaagta gtcctgaact actgccttct ggtgtgactg
1381atgaaaatga ggtgactaca gctgttactg aaaaagtttg ttctgaactc gacaataata
1441gacattcaaa agaggaagat ccatttaatg tagactcaag ttcgctgaca ggtccagttg
1501cagatacaaa cttggatttt ttccagtctg atccttttgt tggcagtgat cctttcaagg
1561atgatccttt tggaaaaatc gatccatttg gtggtgatcc tttcaaaggt tcagatccat
1621ttgcatcaga ctgtttcttc aggcaatcta ctgatccttt tgccacttca agcactgacc
1681ctttcagtgc agccaacaat agcagtatta catcggtaga aacgttgaag cacaatgatc
1741cttttgctcc tggtggaaca gttgttgcag caagcgattc agccacagac ccctttgctt
1801ctgtttttgg gaatgaatca tttggaggtg gatttgctga cttcagcaca ttgtcaaagg
1861tcaacaatga agatcctttt cgttcagcca catcgagctc tgtcagcaac gtagtgatta
1921caaaaaatgt atttgaggaa acatcggtca aaagtgaaga tgaaccccca gcactgccac
1981caaagatcgg aactccaaca agaccctgcc ctctaccacc tgggaaaaga tccatcaaca
2041aattggattc tcctgatccc tttaaactga atgatccatt tcagcctttc ccaggcaacg
2101atagccccaa agaaaaagat cctgaaatgt tttgtgatcc attcacttct gctactacca
2161ctaccaataa agaggctgat ccaagcaatt ttgccaactt cagtgcttat ccctctgaag
2221aagatatgat cgaatgggcc aagagggaaa gtgagagaga ggaagagcag aggcttgccc
2281gactaaatca gcaggaacaa gaagacttag aactggctat tgcactcagc aaatctgaga
2341tatagaagca tgcccaactt tcttgtacaa agtggttggt aagcctatcc ctaaccctct
2401cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac
2461aatcaacctc tggatta

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.