Construct: ORF ccsbBroad304_13891

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF002052.1_s304c1, BRDN0000410348
Derived from:
ccsbBroadEn_13891
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
LCK (3932)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13891
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 3932 LCK LCK proto-oncogene, Src fam... NM_001042771.2 94.3% 93.6% 9T>C;963_964ins90;1516C>T
2 human 3932 LCK LCK proto-oncogene, Src fam... NM_005356.5 94.3% 93.6% 9T>C;963_964ins90;1516C>T
3 human 3932 LCK LCK proto-oncogene, Src fam... XM_024447046.1 85.1% 84.5% (many diffs)
4 human 3932 LCK LCK proto-oncogene, Src fam... XM_024447047.1 85.1% 84.5% (many diffs)
5 human 3932 LCK LCK proto-oncogene, Src fam... NM_001330468.2 84.8% 84% (many diffs)
6 human 3932 LCK LCK proto-oncogene, Src fam... XM_011541453.3 76.6% 75.8% (many diffs)
7 mouse 16818 Lck lymphocyte protein tyrosine... NM_001162433.1 83.5% 90.7% (many diffs)
8 mouse 16818 Lck lymphocyte protein tyrosine... NM_010693.3 83.5% 90.7% (many diffs)
9 mouse 16818 Lck lymphocyte protein tyrosine... XM_006502818.3 83.5% 90.7% (many diffs)
10 mouse 16818 Lck lymphocyte protein tyrosine... XM_030253275.1 83.5% 90.7% (many diffs)
11 mouse 16818 Lck lymphocyte protein tyrosine... XM_030253276.1 83.5% 90.7% (many diffs)
12 mouse 16818 Lck lymphocyte protein tyrosine... NM_001162432.1 81.9% 88.9% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1671
ORF length:
1605
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggg ctgcggctgc agctcacacc cggaagatga ctggatggaa aacatcgatg
121tgtgtgagaa ctgccattat cccatagtcc cactggatgg caagggcacg ctgctcatcc
181gaaatggctc tgaggtgcgg gacccactgg ttacctacga aggctccaat ccgccggctt
241ccccactgca agacaacctg gttatcgctc tgcacagcta tgagccctct cacgacggag
301atctgggctt tgagaagggg gaacagctcc gcatcctgga gcagagcggc gagtggtgga
361aggcgcagtc cctgaccacg ggccaggaag gcttcatccc cttcaatttt gtggccaaag
421cgaacagcct ggagcccgaa ccctggttct tcaagaacct gagccgcaag gacgcggagc
481ggcagctcct ggcgcccggg aacactcacg gctccttcct catccgggag agcgagagca
541ccgcgggatc gttttcactg tcggtccggg acttcgacca gaaccaggga gaggtggtga
601aacattacaa gatccgtaat ctggacaacg gtggcttcta catctcccct cgaatcactt
661ttcccggcct gcatgaactg gtccgccatt acaccaatgc ttcagatggg ctgtgcacac
721ggttgagccg cccctgccag acccagaagc cccagaagcc gtggtgggag gacgagtggg
781aggttcccag ggagacgctg aagctggtgg agcggctggg ggctggacag ttcggggagg
841tgtggatggg gtactacaac gggcacacga aggtggcggt gaagagcctg aagcagggca
901gcatgtcccc ggacgccttc ctggccgagg ccaacctcat gaagcagctg caacaccagc
961ggctggttcg gctctacgct gtggtcaccc aggagcccat ctacatcatc actgaataca
1021tggagaatga cacacttcta gactcccagt tggaggagaa aggtctgggg gcctccccct
1081ggggcaactt gggccagcaa ctcttgcttc tgcccacagg gagtctagtg gattttctca
1141agaccccttc aggcatcaag ttgaccatca acaaactcct ggacatggca gcccaaattg
1201cagaaggcat ggcattcatt gaagagcgga attatattca tcgtgacctt cgggctgcca
1261acattctggt gtctgacacc ctgagctgca agattgcaga ctttggccta gcacgcctca
1321ttgaggacaa cgagtacaca gccagggagg gggccaagtt tcccattaag tggacagcgc
1381cagaagccat taactacggg acattcacca tcaagtcaga tgtgtggtct tttgggatcc
1441tgctgacgga aattgtcacc cacggccgca tcccttaccc agggatgacc aacccggagg
1501tgattcagaa cctggagcga ggctaccgca tggtgcgccc tgacaactgt ccagaggagc
1561tgtaccaact catgaggctg tgctggaagg agcgcccaga ggaccggccc acctttgact
1621acctgcgcag tgtgctggag gacttcttca cggccacaga gggccagtac tagcctcagc
1681cttgcccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc
1741tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc
1801tctggatta

Download FASTA (ORF) (Full)