Transcript: Mouse XM_030253276.1

PREDICTED: Mus musculus lymphocyte protein tyrosine kinase (Lck), transcript variant X3, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Mus musculus (mouse)
Gene:
Lck (16818)
Length:
2152
CDS:
174..1703

Additional Resources:

NCBI RefSeq record:
XM_030253276.1
NBCI Gene record:
Lck (16818)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse XM_030253276.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000367976 CACGATCTAGTCCGCCATTAC pLKO_005 780 CDS 100% 10.800 15.120 N Lck n/a
2 TRCN0000024973 CGGCCCACGTTTGACTACCTT pLKO.1 1623 CDS 100% 1.000 1.400 N Lck n/a
3 TRCN0000361248 CACCAGAAGCCATTAACTATG pLKO_005 1396 CDS 100% 10.800 8.640 N Lck n/a
4 TRCN0000024971 GAGAACATTGACGTGTGTGAA pLKO.1 216 CDS 100% 4.950 3.960 N Lck n/a
5 TRCN0000024970 CCCTTCAACTTCGTGGCGAAA pLKO.1 507 CDS 100% 4.050 3.240 N Lck n/a
6 TRCN0000361302 GAACGGGAGCCTAGTAGATTT pLKO_005 1133 CDS 100% 13.200 9.240 N Lck n/a
7 TRCN0000024969 GCTGCAAGACAACCTGGTTAT pLKO.1 353 CDS 100% 10.800 7.560 N Lck n/a
8 TRCN0000378523 TGTGGAGCACGTGAGTCTAAG pLKO_005 1904 3UTR 100% 10.800 7.560 N Lck n/a
9 TRCN0000024972 GCGGTGAAGAGTCTGAAACAA pLKO.1 984 CDS 100% 5.625 3.938 N Lck n/a
Download CSV

shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_030253276.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

Download CSV

All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14688 pDONR223 0% 88.6% 96.8% None (many diffs) n/a
2 ccsbBroad304_14688 pLX_304 26.4% 88.6% 96.8% V5 (many diffs) n/a
3 TRCN0000480260 TAAGTGCCAAACCCGCCCCTCGTC pLX_317 24.6% 88.6% 96.8% V5 (many diffs) n/a
4 TRCN0000488100 TATGTCATCCAAGTGGGACCAATC pLX_317 19.7% 88.6% 96.8% V5 (many diffs) n/a
5 TRCN0000489331 AAAACCCGTACCATGTAATGTTAC pLX_317 23.5% 83.7% 91.4% V5 (not translated due to prior stop codon) (many diffs) n/a
6 TRCN0000491798 CTTCTGGTCGTGTGGGCTGGGTCC pLX_317 18.3% 83.7% 91.4% V5 (not translated due to prior stop codon) (many diffs) n/a
7 ccsbBroadEn_13891 pDONR223 100% 83.5% 90.7% None (many diffs) n/a
8 ccsbBroad304_13891 pLX_304 0% 83.5% 90.7% V5 (not translated due to prior stop codon) (many diffs) n/a
9 TRCN0000479854 GATATTGTCGAACACGCCTCACTG pLX_317 22.9% 83.5% 90.7% V5 (not translated due to prior stop codon) (many diffs) n/a
Download CSV