Construct: ORF ccsbBroad304_13913
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002976.1_s304c1, BRDN0000462247
- Derived from:
- ccsbBroadEn_13913
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NPR1 (4881)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13913
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 4881 | NPR1 | natriuretic peptide receptor 1 | NM_000906.4 | 99.8% | 99.6% | (many diffs) |
| 2 | human | 4881 | NPR1 | natriuretic peptide receptor 1 | XM_005245218.2 | 97.4% | 97.1% | (many diffs) |
| 3 | human | 4881 | NPR1 | natriuretic peptide receptor 1 | XM_017001374.2 | 66.1% | 65.8% | (many diffs) |
| 4 | mouse | 18160 | Npr1 | natriuretic peptide receptor 1 | NM_008727.5 | 86.3% | 90.4% | (many diffs) |
| 5 | mouse | 18160 | Npr1 | natriuretic peptide receptor 1 | XR_375494.1 | 49.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 3249
- ORF length:
- 3183
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc ggggccccgg cgccccgctg gctcccgcct gcgcctgctc ctgctcctgc 121 tgctgccgcc gctgctgctg ctgctccggg gcagccacgc gggcaacctg acggtagccg 181 tggtactgcc gctggccaat acctcgtacc cctggtcgtg ggcgcgcgtg ggacccgccg 241 tggagctggc cctggcccag gtgaaggcgc gccccgactt gctgccgggc tggacggtcc 301 gcacggtgct gggcagcagc gaaaacgcgc tgggcgtctg ctccgacacc gcagcgcccc 361 tggccgcggt ggacctcaag tgggagcaca accccgctgt gttcctgggc cccggctgcg 421 tgtacgccgc cgccccagtg gggcgcttca ccgcgcactg gcgggtcccg ctgctgaccg 481 ccggcgcccc ggcgctgggc ttcggtgtca aggacgagta tgcgctgacc acccgcgcgg 541 ggcccagcta cgccaagctg ggggacttcg tggcggcgct gcaccgacgg ctgggctggg 601 agcgccaagc gctcatgctc tacgcctaca ggccgggtga cgaagagcac tgcttcttcc 661 tcgtggaggg gctgttcatg cgggtccgcg accgcctcaa tattacggtg gaccacctgg 721 agttcgccga ggacgacctc agccactaca ccaggctgct gcggaccatg ccgcgcaaag 781 gccgagttat ctacatctgc agctcccctg atgccttcag aaccctcatg ctcctggccc 841 tggaagctgg cttgtgtggg gaggactacg ttttcttcca cctggatatc tttgggcaaa 901 gcctgcaagg tggacagggc cctgctcccc gcaggccctg ggagagaggg gatgggcagg 961 atgtcagtgc ccgccaggcc tttcaggctg ccaaaatcat tacatataaa gacccagata 1021 atcccgagta cttggaattc ctgaagcagt taaaacacct ggcctatgag cagttcaact 1081 tcaccatgga ggatgtcctg gtgaacacca tcccagcatc cttccacgac gggctcctgc 1141 tctatatcca ggcagtgacg gagactctgg cacatggggg aactgttact gatggggaga 1201 acatcactca gcggatgtgg aaccgaagct ttcaaggtgt gacaggatac ctgaaaattg 1261 atagcagtgg cgatcgggaa acagacttct ccctctggga tatggatccc gagaatggtg 1321 ccttcagggt tgtactgaac tacaatggga cttcccaaga gctggtggct gtgtcggggc 1381 gcaaactgaa ctggcccctg gggtaccctc ctcctgacat ccccaaatgt ggctttgaca 1441 acgaagaccc agcatgcaac caagatcacc tttccaccct ggaggtgctg gctttggtgg 1501 gcagcctctc cttgctcggc attctgattg tctccttctt catatacagg aagatgcagc 1561 tggagaagga actggcctcg gagctgtggc gggtgcgctg ggaggacgtt gagcccagta 1621 gccttgagag gcacctgcgg agtgcaggca gccggctgac cctgagcggg agaggctcca 1681 attacggctc cctgctaacc acagagggcc agttccaagt ctttgccaag acagcatatt 1741 ataagggcaa cctcgtggct gtgaaacgtg tgaaccgtaa acgcattgag ctgacacgaa 1801 aagtcctgtt tgaactgaag catatgcggg atgtgcagaa tgaacacctg accaggtttg 1861 tgggagcctg caccgacccc cccaatatct gcatcctcac agagtactgt ccccgtggga 1921 gcctgcagga cattctggag aatgagagca tcaccctgga ctggatgttc cggtactcac 1981 tcaccaatga catcgtcaag ggcatgctgt ttctacacaa tggggctatc tgttcccatg 2041 ggaacctcaa gtcatccaac tgcgtggtag atgggcgctt tgtgctcaag atcaccgact 2101 atgggctgga gagcttcagg gacctggacc cagagcaagg acacaccgtt tatgccaaaa 2161 agctgtggac ggcccctgag ctcctgcgaa tggcttcacc ccctgtgcgg ggctcccagg 2221 ctggtgacgt atacagcttt gggatcatcc ttcaggagat tgccctgagg agtggggtct 2281 tccacgtgga aggtttggac ctgagcccca aagagatcat cgagcgggtg actcggggtg 2341 agcagccccc cttccggccc tccctggccc tgcagagtca cctggaggag ttggggctgc 2401 tcatgcagcg gtgctgggct gaggacccac aggagaggcc accattccag cagatccgcc 2461 tgacgttgcg caaatttaac agggagaaca gcagcaacat cctggacaac ctgctgtccc 2521 gcatggagca gtacgcgaac aatctggagg aactggtgga ggagcggacc caggcatacc 2581 tggaggagaa gcgcaaggct gaggccctgc tctaccagat cctgcctcac tcagtggctg 2641 agcagctgaa gcgtggggag acggtgcagg ccgaagcctt tgacagtgtt accatctact 2701 tcagtgacat tgtgggtttc acagcgctgt cggcggagag cacgcccatg caggtggtga 2761 ccctgctcaa tgacctgtac acttgctttg atgctgtcat agacaacttt gatgtgtaca 2821 aggtggagac aattggcgat gcctacatgg tggtgtcagg gctccctgtg cggaacgggc 2881 ggctacacgc ctgcgaggta gcccgcatgg ccctggcact gctggatgct gtgcgctcct 2941 tccgaatccg ccaccggccc caggagcagc tgcgcttgcg cattggcatc cacacaggac 3001 ctgtgtgtgc tggagtggtg ggactgaaga tgccccgtta ctgtctcttt ggggatacag 3061 tcaacacagc ctcaagaatg gagtctaatg gggaagccct gaagatccac ttgtcttctg 3121 agaccaaggc tgtcctggag gagtttggtg gtttcgagct ggagcttcga ggggatgtag 3181 aaatgaaggg caaaggcaag gttcggacct actggctcct tggggagagg gggagtagcc 3241 ccgaggctgc ccaactttct tgtacaaagt ggttggtaag cctatcccta accctctcct 3301 cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta agtcgacaat 3361 caacctctgg atta