Construct: ORF ccsbBroad304_13916

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015890.1_s304c1, BRDN0000462029
Derived from:
ccsbBroadEn_13916
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NRCAM (4897)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13916
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4897 NRCAM neuronal cell adhesion mole... NM_001371175.1 70.8% 70.5% (many diffs)
2 human 4897 NRCAM neuronal cell adhesion mole... NM_001371157.1 68.8% 68.5% (many diffs)
3 human 4897 NRCAM neuronal cell adhesion mole... NM_001371150.1 68.4% 68.1% (many diffs)
4 human 4897 NRCAM neuronal cell adhesion mole... XM_024446780.1 67.8% 67.5% (many diffs)
5 human 4897 NRCAM neuronal cell adhesion mole... NM_001371154.1 65.6% 65.3% (many diffs)
6 human 4897 NRCAM neuronal cell adhesion mole... NM_001371166.1 65.6% 65.3% (many diffs)
7 human 4897 NRCAM neuronal cell adhesion mole... NM_001371133.1 64.8% 64.5% (many diffs)
8 human 4897 NRCAM neuronal cell adhesion mole... NM_001371151.1 64.8% 64.5% (many diffs)
9 human 4897 NRCAM neuronal cell adhesion mole... NM_001371171.1 64.8% 64.5% (many diffs)
10 human 4897 NRCAM neuronal cell adhesion mole... NM_001371178.1 64.8% 64.5% (many diffs)
11 human 4897 NRCAM neuronal cell adhesion mole... NM_005010.4 64.8% 64.5% (many diffs)
12 human 4897 NRCAM neuronal cell adhesion mole... NM_001371134.1 64.7% 64.4% (many diffs)
13 human 4897 NRCAM neuronal cell adhesion mole... NM_001371130.1 64.6% 64.4% (many diffs)
14 human 4897 NRCAM neuronal cell adhesion mole... NM_001371152.1 64.6% 64.4% (many diffs)
15 human 4897 NRCAM neuronal cell adhesion mole... NM_001371176.1 64.6% 64.4% (many diffs)
16 human 4897 NRCAM neuronal cell adhesion mole... XM_024446778.1 64.6% 64.3% (many diffs)
17 human 4897 NRCAM neuronal cell adhesion mole... NM_001371174.1 64.5% 64.2% (many diffs)
18 human 4897 NRCAM neuronal cell adhesion mole... NM_001371159.1 64.3% 64% (many diffs)
19 human 4897 NRCAM neuronal cell adhesion mole... XM_017012251.2 64.3% 64% (many diffs)
20 human 4897 NRCAM neuronal cell adhesion mole... XM_024446777.1 64.3% 64% (many diffs)
21 human 4897 NRCAM neuronal cell adhesion mole... XM_017012248.2 64.1% 63.8% (many diffs)
22 human 4897 NRCAM neuronal cell adhesion mole... NM_001371160.1 64% 63.8% (many diffs)
23 human 4897 NRCAM neuronal cell adhesion mole... NM_001371153.1 63.9% 63.7% (many diffs)
24 human 4897 NRCAM neuronal cell adhesion mole... NM_001371161.1 63.9% 63.7% (many diffs)
25 human 4897 NRCAM neuronal cell adhesion mole... XM_011516270.2 63.8% 63.5% (many diffs)
26 human 4897 NRCAM neuronal cell adhesion mole... NM_001371173.1 63.7% 63.5% (many diffs)
27 human 4897 NRCAM neuronal cell adhesion mole... XM_006716014.2 63.7% 63.5% (many diffs)
28 human 4897 NRCAM neuronal cell adhesion mole... XM_017012246.2 63.6% 63.4% (many diffs)
29 human 4897 NRCAM neuronal cell adhesion mole... XM_011516269.2 63.6% 63.3% (many diffs)
30 human 4897 NRCAM neuronal cell adhesion mole... NM_001193582.1 63.3% 63% (many diffs)
31 human 4897 NRCAM neuronal cell adhesion mole... NM_001371128.1 63.3% 63% (many diffs)
32 human 4897 NRCAM neuronal cell adhesion mole... NM_001371144.1 63.3% 63% (many diffs)
33 human 4897 NRCAM neuronal cell adhesion mole... NM_001371168.1 63.3% 63% (many diffs)
34 human 4897 NRCAM neuronal cell adhesion mole... NM_001371127.1 63.2% 63% (many diffs)
35 human 4897 NRCAM neuronal cell adhesion mole... NM_001371123.1 63.1% 62.9% (many diffs)
36 human 4897 NRCAM neuronal cell adhesion mole... NM_001371149.1 63.1% 62.9% (many diffs)
37 human 4897 NRCAM neuronal cell adhesion mole... XM_006716012.2 63.1% 62.8% (many diffs)
38 human 4897 NRCAM neuronal cell adhesion mole... NM_001371182.1 63% 62.8% (many diffs)
39 human 4897 NRCAM neuronal cell adhesion mole... XM_024446779.1 63% 62.8% (many diffs)
40 human 4897 NRCAM neuronal cell adhesion mole... XM_011516267.2 62.9% 62.7% (many diffs)
41 human 4897 NRCAM neuronal cell adhesion mole... NM_001371136.1 62.6% 62.3% (many diffs)
42 human 4897 NRCAM neuronal cell adhesion mole... NM_001193584.1 62.4% 62.1% (many diffs)
43 human 4897 NRCAM neuronal cell adhesion mole... NM_001371162.1 62.4% 62.1% (many diffs)
44 human 4897 NRCAM neuronal cell adhesion mole... XM_011516266.3 62.3% 62.1% (many diffs)
45 human 4897 NRCAM neuronal cell adhesion mole... NM_001371141.1 62.2% 61.9% (many diffs)
46 human 4897 NRCAM neuronal cell adhesion mole... NM_001371129.1 62.1% 61.9% (many diffs)
47 human 4897 NRCAM neuronal cell adhesion mole... NM_001371132.1 62.1% 61.9% (many diffs)
48 human 4897 NRCAM neuronal cell adhesion mole... XM_011516265.3 61.9% 61.6% (many diffs)
49 human 4897 NRCAM neuronal cell adhesion mole... NM_001193583.1 61.7% 61.5% (many diffs)
50 human 4897 NRCAM neuronal cell adhesion mole... NM_001371122.1 61.7% 61.5% (many diffs)
51 human 4897 NRCAM neuronal cell adhesion mole... NM_001371139.1 61.7% 61.5% (many diffs)
52 human 4897 NRCAM neuronal cell adhesion mole... NM_001371155.1 61.7% 61.5% (many diffs)
53 human 4897 NRCAM neuronal cell adhesion mole... NM_001371163.1 61.7% 61.5% (many diffs)
54 human 4897 NRCAM neuronal cell adhesion mole... NM_001371165.1 61.7% 61.5% (many diffs)
55 human 4897 NRCAM neuronal cell adhesion mole... NM_001371167.1 61.7% 61.5% (many diffs)
56 human 4897 NRCAM neuronal cell adhesion mole... NM_001371177.1 61.7% 61.5% (many diffs)
57 human 4897 NRCAM neuronal cell adhesion mole... NM_001371181.1 61.7% 61.5% (many diffs)
58 human 4897 NRCAM neuronal cell adhesion mole... NM_001371135.1 61.6% 61.3% (many diffs)
59 human 4897 NRCAM neuronal cell adhesion mole... XM_017012247.2 61.5% 61.3% (many diffs)
60 human 4897 NRCAM neuronal cell adhesion mole... XM_024446775.1 61.4% 61.1% (many diffs)
61 human 4897 NRCAM neuronal cell adhesion mole... NM_001371138.1 61.3% 61.1% (many diffs)
62 human 4897 NRCAM neuronal cell adhesion mole... NM_001371140.1 60.2% 60% (many diffs)
63 human 4897 NRCAM neuronal cell adhesion mole... NM_001371145.1 59.7% 59.4% (many diffs)
64 human 4897 NRCAM neuronal cell adhesion mole... NM_001371169.1 59.3% 59.1% (many diffs)
65 human 4897 NRCAM neuronal cell adhesion mole... NM_001371158.1 59.2% 59% (many diffs)
66 human 4897 NRCAM neuronal cell adhesion mole... XM_011516261.2 59.2% 58.9% (many diffs)
67 human 4897 NRCAM neuronal cell adhesion mole... XM_006716007.2 59.2% 58.9% (many diffs)
68 human 4897 NRCAM neuronal cell adhesion mole... XM_017012236.2 59.1% 58.8% (many diffs)
69 human 4897 NRCAM neuronal cell adhesion mole... XM_011516259.2 58.9% 58.7% (many diffs)
70 human 4897 NRCAM neuronal cell adhesion mole... NM_001037132.4 58.8% 58.5% (many diffs)
71 human 4897 NRCAM neuronal cell adhesion mole... NM_001371131.1 58.8% 58.5% (many diffs)
72 human 4897 NRCAM neuronal cell adhesion mole... XM_011516257.2 58.7% 58.5% (many diffs)
73 human 4897 NRCAM neuronal cell adhesion mole... XM_011516258.1 58.7% 58.5% (many diffs)
74 human 4897 NRCAM neuronal cell adhesion mole... NM_001371156.1 58.7% 58.4% (many diffs)
75 human 4897 NRCAM neuronal cell adhesion mole... XM_006716003.2 58.6% 58.4% (many diffs)
76 human 4897 NRCAM neuronal cell adhesion mole... XM_011516253.2 58.5% 58.2% (many diffs)
77 human 4897 NRCAM neuronal cell adhesion mole... XM_011516255.1 58.5% 58.2% (many diffs)
78 human 4897 NRCAM neuronal cell adhesion mole... NM_001371146.1 57.9% 57.6% (many diffs)
79 human 4897 NRCAM neuronal cell adhesion mole... NM_001371124.1 57.3% 57.1% (many diffs)
80 human 4897 NRCAM neuronal cell adhesion mole... NM_001371126.1 57.3% 57.1% (many diffs)
81 human 4897 NRCAM neuronal cell adhesion mole... NM_001371172.1 57.3% 57.1% (many diffs)
82 human 4897 NRCAM neuronal cell adhesion mole... XM_017012239.2 57.3% 57% (many diffs)
83 human 4897 NRCAM neuronal cell adhesion mole... NM_001371119.1 57.2% 57% (many diffs)
84 human 4897 NRCAM neuronal cell adhesion mole... XM_017012238.2 57.2% 57% (many diffs)
85 human 4897 NRCAM neuronal cell adhesion mole... NM_001371148.1 57.2% 56.9% (many diffs)
86 human 4897 NRCAM neuronal cell adhesion mole... NM_001371179.1 57.2% 56.9% (many diffs)
87 human 4897 NRCAM neuronal cell adhesion mole... NM_001371180.1 57.2% 56.9% (many diffs)
88 human 4897 NRCAM neuronal cell adhesion mole... XM_024446773.1 57.2% 56.9% (many diffs)
89 human 4897 NRCAM neuronal cell adhesion mole... XM_011516262.2 57% 56.8% (many diffs)
90 human 4897 NRCAM neuronal cell adhesion mole... NM_001371170.1 57% 56.7% (many diffs)
91 human 4897 NRCAM neuronal cell adhesion mole... NM_001371142.1 55% 54.7% (many diffs)
92 human 4897 NRCAM neuronal cell adhesion mole... NM_001371125.1 54.6% 54.3% (many diffs)
93 human 4897 NRCAM neuronal cell adhesion mole... NM_001371147.1 54.6% 54.3% (many diffs)
94 human 4897 NRCAM neuronal cell adhesion mole... XM_011516268.2 51.9% 51.6% (many diffs)
95 human 4897 NRCAM neuronal cell adhesion mole... NM_001371143.1 50.7% 50.4% (many diffs)
96 human 4897 NRCAM neuronal cell adhesion mole... NM_001371164.1 50.7% 50.4% (many diffs)
97 human 4897 NRCAM neuronal cell adhesion mole... NM_001371137.1 39.2% 38.9% (many diffs)
98 human 4897 NRCAM neuronal cell adhesion mole... NR_163870.1 35.8% (many diffs)
99 human 4897 NRCAM neuronal cell adhesion mole... NR_163871.1 35.5% (many diffs)
100 human 4897 NRCAM neuronal cell adhesion mole... NR_163867.1 34.6% (many diffs)
101 human 4897 NRCAM neuronal cell adhesion mole... NR_163869.1 33.8% (many diffs)
102 human 4897 NRCAM neuronal cell adhesion mole... NR_163868.1 33.1% (many diffs)
103 mouse 319504 Nrcam neuronal cell adhesion mole... XM_006515968.2 57% 59.3% (many diffs)
104 mouse 319504 Nrcam neuronal cell adhesion mole... XM_017315104.1 57% 59.3% (many diffs)
105 mouse 319504 Nrcam neuronal cell adhesion mole... NM_001146031.1 56.8% 59.1% (many diffs)
106 mouse 319504 Nrcam neuronal cell adhesion mole... XM_017315103.1 56.7% 59% (many diffs)
107 mouse 319504 Nrcam neuronal cell adhesion mole... XM_017315102.1 56.6% 58.8% (many diffs)
108 mouse 319504 Nrcam neuronal cell adhesion mole... XM_017315100.1 56.4% 58.6% (many diffs)
109 mouse 319504 Nrcam neuronal cell adhesion mole... XM_017315101.1 56.3% 58.6% (many diffs)
110 mouse 319504 Nrcam neuronal cell adhesion mole... XM_006515966.3 56.2% 58.5% (many diffs)
111 mouse 319504 Nrcam neuronal cell adhesion mole... XM_017315098.1 56.1% 58.4% (many diffs)
112 mouse 319504 Nrcam neuronal cell adhesion mole... XM_006515965.2 56% 58.3% (many diffs)
113 mouse 319504 Nrcam neuronal cell adhesion mole... XM_017315099.1 56% 58.2% (many diffs)
114 mouse 319504 Nrcam neuronal cell adhesion mole... XM_006515964.2 55.9% 58.2% (many diffs)
115 mouse 319504 Nrcam neuronal cell adhesion mole... XM_017315096.1 55.8% 58% (many diffs)
116 mouse 319504 Nrcam neuronal cell adhesion mole... XM_017315097.1 55.8% 58% (many diffs)
117 mouse 319504 Nrcam neuronal cell adhesion mole... XM_006515963.2 55.7% 57.9% (many diffs)
118 mouse 319504 Nrcam neuronal cell adhesion mole... XM_017315095.1 55.5% 57.8% (many diffs)
119 mouse 319504 Nrcam neuronal cell adhesion mole... XM_006515962.2 55.5% 57.7% (many diffs)
120 mouse 319504 Nrcam neuronal cell adhesion mole... XM_006515961.3 55.4% 57.6% (many diffs)
121 mouse 319504 Nrcam neuronal cell adhesion mole... XM_006515960.2 54.8% 57% (many diffs)
122 mouse 319504 Nrcam neuronal cell adhesion mole... XM_006515969.2 54.7% 56.9% (many diffs)
123 mouse 319504 Nrcam neuronal cell adhesion mole... XM_006515967.2 54.1% 56.3% (many diffs)
124 mouse 319504 Nrcam neuronal cell adhesion mole... XM_017315094.1 53.8% 56% (many diffs)
125 mouse 319504 Nrcam neuronal cell adhesion mole... NM_176930.4 53.7% 55.8% (many diffs)
126 mouse 319504 Nrcam neuronal cell adhesion mole... XM_017315093.1 53.6% 55.8% (many diffs)
127 mouse 319504 Nrcam neuronal cell adhesion mole... XM_017315092.1 53.4% 55.6% (many diffs)
128 mouse 319504 Nrcam neuronal cell adhesion mole... XM_006515959.2 53.3% 55.4% (many diffs)
129 mouse 319504 Nrcam neuronal cell adhesion mole... XM_006515958.2 53.1% 55.3% (many diffs)
130 mouse 319504 Nrcam neuronal cell adhesion mole... XM_017315089.1 52.2% 54.3% (many diffs)
131 mouse 319504 Nrcam neuronal cell adhesion mole... XM_017315090.1 52.1% 54.2% (many diffs)
132 mouse 319504 Nrcam neuronal cell adhesion mole... XM_006515956.2 52% 54.1% (many diffs)
133 mouse 319504 Nrcam neuronal cell adhesion mole... XM_006515955.2 51.9% 54% (many diffs)
134 mouse 319504 Nrcam neuronal cell adhesion mole... XM_006515954.2 51.8% 53.9% (many diffs)
135 mouse 319504 Nrcam neuronal cell adhesion mole... XM_006515953.3 51.7% 53.8% (many diffs)
136 mouse 319504 Nrcam neuronal cell adhesion mole... XM_006515950.2 51.7% 53.7% (many diffs)
137 mouse 319504 Nrcam neuronal cell adhesion mole... XM_006515951.2 51.7% 53.7% (many diffs)
138 mouse 319504 Nrcam neuronal cell adhesion mole... XM_006515952.3 51.7% 53.7% (many diffs)
139 mouse 319504 Nrcam neuronal cell adhesion mole... XM_006515949.2 51.6% 53.7% (many diffs)
140 mouse 319504 Nrcam neuronal cell adhesion mole... XM_006515948.2 51.6% 53.6% (many diffs)
141 mouse 319504 Nrcam neuronal cell adhesion mole... XM_006515944.2 51.4% 53.5% (many diffs)
142 mouse 319504 Nrcam neuronal cell adhesion mole... XM_006515945.2 51.4% 53.5% (many diffs)
143 mouse 319504 Nrcam neuronal cell adhesion mole... XM_006515946.3 51.4% 53.5% (many diffs)
144 mouse 319504 Nrcam neuronal cell adhesion mole... XM_006515947.3 51.4% 53.5% (many diffs)
145 mouse 319504 Nrcam neuronal cell adhesion mole... XM_017315091.1 50.1% 52.2% (many diffs)
146 mouse 319504 Nrcam neuronal cell adhesion mole... XM_006515957.2 50% 52% (many diffs)
147 mouse 319504 Nrcam neuronal cell adhesion mole... XR_381525.3 45.8% (many diffs)
148 mouse 319504 Nrcam neuronal cell adhesion mole... XR_381526.2 18.5% (many diffs)
149 mouse 319504 Nrcam neuronal cell adhesion mole... XR_001780452.1 18.5% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
2382
ORF length:
2313
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gcagcttaaa ataatgccga aaaagaagcg cttatctgcg ggcagagtgc
121ccctgattct cttcctgtgc cagatgatta gtgcactgga agtacctctt gatctggtac
181agcctccaac catcacccaa cagtctccaa aagattacat tattgaccct cgggagaata
241ttgtaatcca gtgtgaagcc aaagggaaac cgcccccaag cttttcctgg acccgtaatg
301ggactcattt tgacatcgat aaagaccctc tggtcaccat gaagcctggc acaggaacgc
361tcataattaa catcatgagc gaagggaaag ctgagaccta tgaaggagtc tatcagtgta
421cagcaaggaa cgaacgcgga gctgcagttt ctaataacat tgttgtccgc ccatccagat
481caccattgtg gaccaaagaa aaacttgaac caatcacact tcaaagtggt cagtctttag
541tacttccctg cagaccccca attggattac caccacctat aatattttgg atggataatt
601cctttcaaag acttccacaa agtgagagag tttctcaagg tttgaatggg gacctttatt
661tttccaatgt cctcccagag gacacccgcg aagactatat ctgttatgct agatttaatc
721atactcaaac catacagcag aagcaaccta tttctgtgaa ggtgatttca gtggatgaat
781tgaatgacac tatagctgct aatttgagtg acactgagtt ttatggtgct aaatcaagta
841gagagaggcc accaacattt ttaactccag aaggcaatgc aagtaacaaa gaggaattaa
901gaggaaatgt gctttcactg gagtgcattg cagaaggact gcctacccca attatttact
961gggcaaagga agatggaatg ctacccaaaa acaggacagt ttataagaac tttgagaaaa
1021ccttgcagat cattcatgtt tcagaagcag actctggaaa ttaccaatgt atagcaaaaa
1081acgcattagg agccatccac cataccattt ctgttagagt taaagcggct ccatactgga
1141tcacagcccc tcaaaatctt gtgctgtccc caggagagga tgggaccttg atctgcagag
1201ctaatggcaa ccccaaaccc agaattagct ggttaacaaa tggagtccca atagaaattg
1261cccctgatga ccccagcaga aaaatagatg gcgataccat tattttttca aatgttcaag
1321aaagatcaag tgcagtatat cagtgcaatg cctctaatga atatggatat ttactggcaa
1381acgcatttgt aaatgtgctg gctgagccac cacgaatcct cacacctgca aacacactct
1441accaggtcat tgcaaacagg cctgctttac tagactgtgc cttctttggg tctcctctcc
1501caaccatcga gtggtttaaa ggagctaaag gaagtgctct tcatgaagat atttatgttt
1561tacatgaaaa tggaactttg gaaattcctg tggcccaaaa ggacagtaca ggaacttata
1621cgtgtgttgc aaggaataaa ttagggatgg caaagaatga agttcactta gaaatcaaag
1681atgctacatg gatcgttaaa cagcccgaat atgcagttgt gcaaagaggg agcatggtgt
1741cctttgaatg caaagtgaaa catgatcaca ccttatccct cactgtcctg tggctgaagg
1801acaacaggga actgcccagt gatgaaaggt tcactgttga caaggatcat ctagtggtag
1861ctgatgtcag tgacgatgac agcgggacct acacgtgtgt ggccaacacc actctggaca
1921gcgtctccgc cagcgctgtg cttagcgttg ttgatgtccc aaatcctccc tttgacttag
1981aactgacaga tcaacttgac aaaagtgttc agctgtcatg gaccccaggc gatgacaaca
2041atagccccat tacaaaattc atcatcgaat atgaagatgc aatgcacaag ccagggctgt
2101ggcaccacca aactgaagtt tctggaacac agaccacagc ccagctgaag ctgtctcctt
2161acgtgaacta ctccttccgc gtgatggcag tgaacagcat tgggaagagc ttgcccagcg
2221aggcctctga gcagtatttg acgaaagcct cagaaccaga taaaaacccc acagctgtgg
2281aaggactggg atcagagcct gataatttgg tgattacgtg gaagcccttg aatggtttcg
2341aatctaatgg gccaggcctt cagacaagta ctgcctcttc tttgccaact ttcttgtaca
2401aagtggttgg taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag
2461ctagcgctaa ccggtggcgc gttaagtcga caatcaacct ctggatta

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