Construct: ORF ccsbBroad304_13920

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018549.1_s304c1, BRDN0000462370
Derived from:
ccsbBroadEn_13920
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
PLCL1 (5334)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13920
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5334 PLCL1 phospholipase C like 1 (ina... XM_017004340.2 99.8% .8% 11delA;1705G>A;1745T>A
2 human 5334 PLCL1 phospholipase C like 1 (ina... XM_005246644.4 98% .7% (many diffs)
3 human 5334 PLCL1 phospholipase C like 1 (ina... XM_011511351.2 98% .7% (many diffs)
4 human 5334 PLCL1 phospholipase C like 1 (ina... XM_017004339.2 98% .7% (many diffs)
5 human 5334 PLCL1 phospholipase C like 1 (ina... XM_005246643.4 97.5% .7% (many diffs)
6 human 5334 PLCL1 phospholipase C like 1 (ina... NM_006226.4 90.9% .7% (many diffs)
7 mouse 227120 Plcl1 phospholipase C-like 1 NM_001114663.1 80.1% .7% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
135
ORF length:
66
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gccatcggaa agaaaattag cagtgcaaat gactgcatca gcttcatgca
121agctggctgt gagttgaaga aagtccggcc aaattctcgc atttacaacc gttttttcac
181tctggacaca gaccttcaag ctcttcgctg ggaaccttca aagaaagacc tcgagaaagc
241caagcttgat atttctgcca taaaagagat cagactgggg aaaaacacgg aaacatttag
301aaacaatggc cttgctgacc agatctgtga ggactgtgcc ttttccatac tccacgggga
361aaactatgag tctctggacc tagttgccaa ttcagcagat gtggcaaaca tctgggtgtc
421tgggttacgg tacctggttt ctcgaagtaa gcagcctctt gattttatgg agggcaacca
481gaacacacca cggttcatgt ggttgaaaac agtgtttgaa gcagcagatg ttgatgggaa
541tgggattatg ttggaagaca cctctgtaga gttaataaaa caactcaacc ctactctgaa
601ggaagccaag atcaggttaa agtttaaaga aatccagaag agcaaggaaa aactaaccac
661ccgcgtgacc gaagaggaat tttgtgaagc tttttgtgaa ctttgcacca ggccagaagt
721gtatttctta cttgtacaga tatctaaaaa caaagaatat ttggatgcca atgatctcat
781gctcttttta gaagctgagc aaggagtcac ccatatcacc gaggatatat gcttagacat
841cataaggaga tacgaacttt ctgaagaggg acgtcaaaaa gggtttcttg caattgatgg
901ctttacccag tatttattgt catcagaatg tgacattttt gatcctgagc aaaagaaggt
961tgcccaagat atgacccagc cattatctca ctactatatc aatgcctctc ataacaccta
1021tctaatagaa gaccagttca gggggccagc tgacatcaat gggtacatta gagctttgaa
1081aatgggctgt cgaagcgttg aactcgatgt aagtgatggt tcagataatg aaccaatcct
1141ttgtaatcga aataacatga caacccatgt ttcctttcga agtgtcatag aggtaataaa
1201taaatttgcc tttgttgctt ctgaataccc actcattctt tgcttgggaa atcactgctc
1261cttgccgcag cagaaggtaa tggctcaaca gatgaaaaag gtctttggca ataaactcta
1321tactgaagca cctttgccct cagaatccta cctcccatca ccagaaaaat taaaaagaat
1381gatcattgtg aaaggaaaga agttgccttc tgatccagat gtgttagaag gagaagtaac
1441agatgaagat gaagaagctg aaatgtctcg aaggatgtcg gtagattaca atggtgagca
1501gaagcaaatc cgactctgta gggagctctc tgatttggtg tctatttgta aatctgttca
1561atacagggat tttgaactat ctatgaaaag ccaaaactat tgggaaatgt gttcatttag
1621tgaaacagag gccagccgca ttgcaaatga gtacccagag gattttgtta attataataa
1681gaagttctta tcaagaatct atccaagtgc catgaggatc gattccagta acttgaatcc
1741acaggacttt tggaattgtg gctgtcagat tatagcaatg aattttcaga ctccgggtcc
1801aatgatggac catcacacgg gctggtttct tcaaaacggg ggatgtggtt atgttctaag
1861gccgtctata atgcgagatg aagtttctta cttcagcgca aatacaaagg gcattctacc
1921tggggtgtct cctctagctc ttcatatcaa gatcatcagt ggtcagaatt tcccaaagcc
1981caagggagct tgtgccaaag gggatgtcat agatccctat gtttgtatag agatacacgg
2041aattccagcg gattgttcgg aacaaagaac taaaactgta cagcaaaaca gtgataatcc
2101tatttttgat gaaacttttg agttccaagt aaacctacct gagctggcca tgatccgttt
2161tgttgttctg gatgatgact acattgggga tgagtttata gggcaatata cgataccatt
2221tgaatgtttg cagcctggat atcggcatgt tcccctgcgt tcttttgtgg gtgacatcat
2281ggagcacgta accctttttg tccacatagc aataactaat cgaagtggag gaggaaaggc
2341acagaagcgc agtctttcag tgagaatggg gaagaaagtt cgggaatata ccatgctcag
2401gaatatcggt cttaaaacca ttgatgacat ctttaaaata gcggttcatc cattacgaga
2461agccatagat atgagagaaa atatgcagaa tgcaatcgtg tctattaagg aactatgtgg
2521actccctcca attgccagtc tgaagcagtg cctgttaact ctgtcatctc ggctcatcac
2581cagtgacaat actccttcag tctcacttgt gatgaaagac agctttcctt acctggagcc
2641tctgggtgca attccagatg tgcagaaaaa gatgctgact gcttatgatc tgatgattca
2701agagagccgg tttctcatag aaatggcgga cacagtccag gaaaagattg tacagtgtca
2761gaaagcaggg atggagttcc atgaagaact tcataatttg ggggcaaaag aaggcttgaa
2821gggaagaaaa ctcaacaaag caactgagag ctttgcttgg aacattacag tattgaaggg
2881ccaaggagat ctgttgaaga atgccaagaa tgaagctata gaaaacatga agcagatcca
2941gctggcatgc ctgtcctgtg gactgagtaa agcccccagc agcagtgctg aggccaagag
3001caagcgcagc ctggaagcca tagaggagaa ggaaagtagt gaggagaatg ggaagctgtt
3061gccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc tcggtctcga
3121ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg
3181gatta

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.