Construct: ORF ccsbBroad304_13920

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018549.1_s304c1, BRDN0000462370
Derived from:
ccsbBroadEn_13920
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
PLCL1 (5334)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13920
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 5334 PLCL1 phospholipase C like 1 (ina... XM_017004340.2 99.8% .8% 11delA;1705G>A;1745T>A
2 human 5334 PLCL1 phospholipase C like 1 (ina... XM_005246644.4 98% .7% (many diffs)
3 human 5334 PLCL1 phospholipase C like 1 (ina... XM_011511351.2 98% .7% (many diffs)
4 human 5334 PLCL1 phospholipase C like 1 (ina... XM_017004339.2 98% .7% (many diffs)
5 human 5334 PLCL1 phospholipase C like 1 (ina... XM_005246643.4 97.5% .7% (many diffs)
6 human 5334 PLCL1 phospholipase C like 1 (ina... NM_006226.4 90.9% .7% (many diffs)
7 mouse 227120 Plcl1 phospholipase C-like 1 NM_001114663.1 80.1% .7% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
135
ORF length:
66
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gccatcggaa agaaaattag cagtgcaaat gactgcatca gcttcatgca
121agctggctgt gagttgaaga aagtccggcc aaattctcgc atttacaacc gttttttcac
181tctggacaca gaccttcaag ctcttcgctg ggaaccttca aagaaagacc tcgagaaagc
241caagcttgat atttctgcca taaaagagat cagactgggg aaaaacacgg aaacatttag
301aaacaatggc cttgctgacc agatctgtga ggactgtgcc ttttccatac tccacgggga
361aaactatgag tctctggacc tagttgccaa ttcagcagat gtggcaaaca tctgggtgtc
421tgggttacgg tacctggttt ctcgaagtaa gcagcctctt gattttatgg agggcaacca
481gaacacacca cggttcatgt ggttgaaaac agtgtttgaa gcagcagatg ttgatgggaa
541tgggattatg ttggaagaca cctctgtaga gttaataaaa caactcaacc ctactctgaa
601ggaagccaag atcaggttaa agtttaaaga aatccagaag agcaaggaaa aactaaccac
661ccgcgtgacc gaagaggaat tttgtgaagc tttttgtgaa ctttgcacca ggccagaagt
721gtatttctta cttgtacaga tatctaaaaa caaagaatat ttggatgcca atgatctcat
781gctcttttta gaagctgagc aaggagtcac ccatatcacc gaggatatat gcttagacat
841cataaggaga tacgaacttt ctgaagaggg acgtcaaaaa gggtttcttg caattgatgg
901ctttacccag tatttattgt catcagaatg tgacattttt gatcctgagc aaaagaaggt
961tgcccaagat atgacccagc cattatctca ctactatatc aatgcctctc ataacaccta
1021tctaatagaa gaccagttca gggggccagc tgacatcaat gggtacatta gagctttgaa
1081aatgggctgt cgaagcgttg aactcgatgt aagtgatggt tcagataatg aaccaatcct
1141ttgtaatcga aataacatga caacccatgt ttcctttcga agtgtcatag aggtaataaa
1201taaatttgcc tttgttgctt ctgaataccc actcattctt tgcttgggaa atcactgctc
1261cttgccgcag cagaaggtaa tggctcaaca gatgaaaaag gtctttggca ataaactcta
1321tactgaagca cctttgccct cagaatccta cctcccatca ccagaaaaat taaaaagaat
1381gatcattgtg aaaggaaaga agttgccttc tgatccagat gtgttagaag gagaagtaac
1441agatgaagat gaagaagctg aaatgtctcg aaggatgtcg gtagattaca atggtgagca
1501gaagcaaatc cgactctgta gggagctctc tgatttggtg tctatttgta aatctgttca
1561atacagggat tttgaactat ctatgaaaag ccaaaactat tgggaaatgt gttcatttag
1621tgaaacagag gccagccgca ttgcaaatga gtacccagag gattttgtta attataataa
1681gaagttctta tcaagaatct atccaagtgc catgaggatc gattccagta acttgaatcc
1741acaggacttt tggaattgtg gctgtcagat tatagcaatg aattttcaga ctccgggtcc
1801aatgatggac catcacacgg gctggtttct tcaaaacggg ggatgtggtt atgttctaag
1861gccgtctata atgcgagatg aagtttctta cttcagcgca aatacaaagg gcattctacc
1921tggggtgtct cctctagctc ttcatatcaa gatcatcagt ggtcagaatt tcccaaagcc
1981caagggagct tgtgccaaag gggatgtcat agatccctat gtttgtatag agatacacgg
2041aattccagcg gattgttcgg aacaaagaac taaaactgta cagcaaaaca gtgataatcc
2101tatttttgat gaaacttttg agttccaagt aaacctacct gagctggcca tgatccgttt
2161tgttgttctg gatgatgact acattgggga tgagtttata gggcaatata cgataccatt
2221tgaatgtttg cagcctggat atcggcatgt tcccctgcgt tcttttgtgg gtgacatcat
2281ggagcacgta accctttttg tccacatagc aataactaat cgaagtggag gaggaaaggc
2341acagaagcgc agtctttcag tgagaatggg gaagaaagtt cgggaatata ccatgctcag
2401gaatatcggt cttaaaacca ttgatgacat ctttaaaata gcggttcatc cattacgaga
2461agccatagat atgagagaaa atatgcagaa tgcaatcgtg tctattaagg aactatgtgg
2521actccctcca attgccagtc tgaagcagtg cctgttaact ctgtcatctc ggctcatcac
2581cagtgacaat actccttcag tctcacttgt gatgaaagac agctttcctt acctggagcc
2641tctgggtgca attccagatg tgcagaaaaa gatgctgact gcttatgatc tgatgattca
2701agagagccgg tttctcatag aaatggcgga cacagtccag gaaaagattg tacagtgtca
2761gaaagcaggg atggagttcc atgaagaact tcataatttg ggggcaaaag aaggcttgaa
2821gggaagaaaa ctcaacaaag caactgagag ctttgcttgg aacattacag tattgaaggg
2881ccaaggagat ctgttgaaga atgccaagaa tgaagctata gaaaacatga agcagatcca
2941gctggcatgc ctgtcctgtg gactgagtaa agcccccagc agcagtgctg aggccaagag
3001caagcgcagc ctggaagcca tagaggagaa ggaaagtagt gaggagaatg ggaagctgtt
3061gccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc tcggtctcga
3121ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg
3181gatta

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